#SmoothMuscleCell⏫
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#SmoothMuscleCell Epitranscriptomics

METTL3-YTHDC1 (m6A reader) ⏫SGK1 mRNA synthesis (but not stability)

135 METTL3 target genes by RNAseq+MeRIPseq in rat SMC

SMC Mettl3 KO🐭➡️
⏬carotid #IntimalHyperplasia➡️
⏫by AAV9-SM22a-SGK1

#ATVB 2025 @ahascience.bsky.social
www.ahajournals.org/doi/10.1161/...
November 6, 2025 at 7:52 PM
haoyin.bsky.social
GPR153-cAMP/CREB-#YAP/TAZ or -NFκB cascades in #EndothelialCell #SmoothMuscleCell

GPR153
⏫in SMC in intima, atheroma, MI ischemic zone
⏫in EC in inflamed SkM, Brain

Myh11+ SMC Gpr153 KO 🐭
Injured artery

Slco1c1+ brain EC Gpr153 KO 🐭
EAE & Stroke

#NatComm 2025
www.nature.com/articles/s41...
October 25, 2025 at 11:21 AM
haoyin.bsky.social
#SmoothMuscleCell m6A Epitranscriptomics

METTL3 as a direct target gene of YY1

METTL3-IGF2BP1 stabilizes MYH11 MYLK2 TGFβ2 mRNAs

SMC Yy1 or Mettl3 KO🐭
⏬vasoconstriction
↔️vasorelaxation

AAV-SM22-METTL2 iv ⏫arterial contractility of SMC Yy1 KO🐭

#CardiovascRes 2025
academic.oup.com/cardiovascre...
October 24, 2025 at 6:55 PM
haoyin.bsky.social
#VascularCalcification Mechanobiology

#HydrostaticPressure 120 mmHg 7 d➡️
⏫#Piezo1➡️ (⏫ #ERStress GPR78 CHOP➡️)
⏫ #SmoothMuscleCell Calcification (~20% level by 4 mM Pi)

#BBAMolBasisDis 2025
www.sciencedirect.com/science/arti...
October 20, 2025 at 11:33 AM
haoyin.bsky.social
Histone Variants
#SmoothMuscleCell

1⃣H2A.Z
⬇️in #Atherosclerosis
⏫SMC contractile gene
#CircAHA 2018
www.ahajournals.org/doi/10.1161/...
#BMCGenom 2025
bmcgenomics.biomedcentral.com/articles/10....

2⃣H3.3B
⬆️in #AorticDissection
⏫SMC synthetic genes
#Genomics 2023
www.sciencedirect.com/science/arti...
October 15, 2025 at 9:16 PM
haoyin.bsky.social
#SmoothMuscleCell m6A Epitranscriptomics

METTL3⏫in👤stenotic #ArterioVenousFistula

SMC Mettl3 KO🐭
⏬AVF Intima

Mettl3-YTHDF1 stabilizes SLC7A11 mRNA➡️
⏬SMC contractile phenotype
⏫SMC proliferation/migration
⏬Lipid peroxidation

#ATVB 2025 @ahajournals.bsky.social
www.ahajournals.org/doi/full/10....
October 13, 2025 at 7:04 PM
haoyin.bsky.social
#vascularCalcification

DNA N6-methyladenine/6mA (NOT m6A) of BMP2 gene ⏬OCT4-dependent transcription & #SmoothMuscleCell Calcification

ALKBH1, an eraser of 6mA
⬇️in calcified radial artery of👤CKD
⏫aortic calcification in 🐭+Adenine diet

@jci.org 2021
www.jci.org/articles/vie...
October 12, 2025 at 6:58 PM
haoyin.bsky.social
#SmoothMuscleCell #Piezo1 #Hypertension

#HydrostaticPressure➡️
⏫GPR146➡️
⏫SMC synthetic phenotype
⏫cAMP-CREB-Piezo1➡️⏫Syst/Diastolic Blood Pressure

Evidence from GPR146 KO
SMC-GPR146 KI & KO
AAV2-SM22a-GPR146 +SMC-Piezo1 KO🐭

#CircRes 2025 @ahajournals.bsky.social
www.ahajournals.org/doi/10.1161/...
September 21, 2025 at 8:01 PM
haoyin.bsky.social
#CircularRNA-derived peptide 😍 in #SmoothMuscleCell

circSETD2(14,15), an evolutionarily conserved circRNA, encodes a peptide (p-414aa👤 p-298aa🐭)➡️
dissociates HuR from c-Fos mRNA➡️
⏬SMC proliferation
⏫SMC contractile phenotype (via activating Myocd?🤔)

#Circ 2025
www.ahajournals.org/doi/10.1161/...
March 26, 2025 at 7:21 PM
haoyin.bsky.social
Evidence for an active role of #EndothalialCell in #Aortopathy

EC TRPV4 activity⏫
🐭#AbdominalAorticAneurysm (local elastase)
via⏫EC #Apoptosis (how does this propagate to #SmoothMuscleCell loss) w/o altering #NitricOxide

#AortaEd
#EurJPharmacol 2024
www.sciencedirect.com/science/arti...
November 28, 2024 at 8:57 PM
haoyin.bsky.social
#YAP coordinates with Phospholamban (a #SmoothMuscleCell contractile protein)➡️Hypertrophy (⏫Cell Area) of Esophageal SMCs

Sorry no TAZ data😔

Guess this cascade may also work in Hypertensive SMC Hypertrophy & Collagen deposit?😁

#MucosalImmunol 2024
www.sciencedirect.com/science/arti...
March 10, 2024 at 12:27 PM
haoyin.bsky.social
Monocyte➡️ #SmoothMuscleCell #EndothelialCell phenotypic shift in #AorticDissection

Lyz2-CreERT2:Basp1 f/f or AAV8-shBasp1🐭+BAPN
👉abrogate thoracic aortic dilation
👉50~80%⏬ #AorticDissection Mortality

In vitro BASP1+ monocyte
⏫ SMC EC Apoptosis

#JAdvRes 2025
www.sciencedirect.com/science/arti...
April 21, 2025 at 7:03 PM
haoyin.bsky.social
#SmoothMuscleCell DOT1L, H3K79 methyltransferase, KO🐭
⏬lesion burden, Mφ
⏫plaque stability

DOT1L ⬆️in SMC+PDGFBB, 👤carotid #Atherosclerosis, 👤popliteal arterial aneursym

shDOT1L in SMC➡️
⏫contractile phenotype

75% 1-h PDGFBB-activated genes are H3K79me2+

#EHJ 2022
academic.oup.com/eurheartj/ar...
October 18, 2025 at 7:20 PM
haoyin.bsky.social
Itga8+ vascular #SmoothMuscleCell #YAP/TAZ KO->
Cartilage in Aorta😆

⏫cGAS-STING signals
Loss of contractile phenotype
#AorticAneurysm (but low rupture) as early as 2 wk, starting from abdominal part

Karl Swärd, Sebastian Albinsson labs JCI Insight 2023
insight.jci.org/articles/vie...
September 10, 2023 at 7:33 PM
haoyin.bsky.social
#Autophagy defect (Atg5 KO) & #ERStress in #SmoothMuscleCell⏫#AorticDissection #AorticRupture in🐭+Angiotensin II

Both features (LC3B, p62, GRP78/BiP) also⏫in👤#ThoracicAorticDissection

#ClonalExpansion of SMC in dissecting🐭aorta🤠
#ATVB 2019
www.ahajournals.org/doi/full/10....
May 26, 2024 at 10:59 AM
haoyin.bsky.social
#hiPSC-derived #EndothelialCell #SmoothMuscleCell #Pericyte model vascular responses to #SARSCOV2 infection

SMC is the most robust responder😎
👉⏫IFN-α/γ signals, Tissue factor activity
👉SMC paracrine➡️
⏫EC permeability, PAI1 vWF release

@naturecomms.bsky.social 2024
www.nature.com/articles/s41...
January 4, 2025 at 1:55 PM
haoyin.bsky.social
#SmoothMuscleCell-secreted small #ExtracellularVesicle/#Migrasome

⏫by Fibronectin-β1 integrin outside-in signal
⏫its migration velocity + modestly directionality
FN-immobilized sEV can't be uptaken by SMC🤓

Dr. Catherine Shanahan lab bioRxiv 2023
www.biorxiv.org/content/10.1...
October 2, 2023 at 7:20 PM
haoyin.bsky.social
Liver-to-Aorta communication😎
#Atheroasclerosis #MAFLD #MedialArterialCalcification

Steatotic Liver-derived #ExtracellularVesicle
⏫atherosclerotic lesion +calcification
⏫MAFLD-associated arterial calcification
⏫Osteogenesis of #SmoothMuscleCell

#AdvSci 2024
onlinelibrary.wiley.com/doi/10.1002/...
January 1, 2025 at 4:13 PM
haoyin.bsky.social
Global KO of Sigmar1🐭, a pleotropic chaperone protein,▶️#EndothelialCell & #SmoothMuscleCell dysfunction

⏫#ArterialStiffness
eNOS deficiency
⏬FMD
SMC disarray
Aortic Fibrosis
Mitochondrial elongation
⏬Mitochondrial respiration

#FrontPhysiol 2024
www.frontiersin.org/journals/phy...
August 11, 2024 at 7:31 PM
haoyin.bsky.social
PCSK9⏬YAP pSer127 & ⏫YAP level in cultured aortic #SmoothMuscleCell!

Functional outcome?😬 And more data,pls😀

PCSK9 immunoreactivity in👤carotid artery atherosclerosis, preferred in cap/shoulder SMCs, ➕corr. with #WallShearStress

#VMJ_SVM 2024
journals.sagepub.com/doi/10.1177/...
June 18, 2024 at 7:19 PM
haoyin.bsky.social
NRIP2+ #SmoothMuscleCell (likely mediating metabolic resilience by Leptin signaling) #AorticDissection

Leptin antagonist R128Q or AAV9-SM22a-NRIP2
⏬🐭AD #AorticRupture (BAPN)
⏫contractile SMC
⏫SMC mitochondrial ultrastructure & OXPHOS

#AdvSci 2025
advanced.onlinelibrary.wiley.com/doi/10.1002/...
November 5, 2025 at 8:16 PM
haoyin.bsky.social
DDX21/DEAD Box Helicase 21 as a ➕regulator of myometrial #SmoothMuscleCell contraction

A long list of DDXs⏫in labouring myometrium!😮(Fig 1A)

DDX21 RNAIP-seq▶️
901 mRNAs in Focal adhesion/ECM/TNFα/NFkB/TGFβ signaling

THBS1 downstream of DDX21

#JCMM 2024
onlinelibrary.wiley.com/doi/10.1111/...
January 9, 2025 at 12:49 PM
haoyin.bsky.social
#SmoothMuscleCell #YAP/TAZ senses Matrix Topology (Flat, Cross-patterned, Line, Curved) (PMMA GPa; PDMS MPa)

2 segregatable Muscularization programs in SMCs？🤔
👉SM α-actin-based, ⏫by verteporfin (YAPi)
👉Calponin-based, ⏬by verteporfin

#AdvHealthcMater 2024
onlinelibrary.wiley.com/doi/10.1002/...
October 27, 2024 at 7:30 PM
haoyin.bsky.social
#SpatialTranscriptomics #Atherosclerosis

Mapping the Senescence (SenMayo CellAge) in p16 reporter🐭aortic atherosclerotic lesions
scRNAseq GeoMx
🔥 #SmoothMuscleCell Fibroblast T

#Senolytic ABT-737 ⏫plaque collagen fibers ⏬necrotic core ⏬serum cytokines

#NatAging 2025
www.nature.com/articles/s43...
July 15, 2025 at 7:17 PM
haoyin.bsky.social
#HGPS #Aortopathy

The age-related accumulation of immature Lamin A, #Progerin or Farnesyl-Prelamin A, ⏫Stretch-induced loss of aortic #SmoothMuscleCell

Comparative analysis among
1⃣LmnaG609G/+
2⃣Zmpste24-/-
3⃣SM22a-Cre::Zmpste f/f::LmnaPLAO/PLAO🐭

#CellDeathDis 2025
www.nature.com/articles/s41...
July 28, 2025 at 7:04 PM