Researchers developed bis-pseudoindoxyls, compact fluorophores with red-shifted absorption and fluorescence, which can be utilized for time-resolved bioimaging Fluorescent dyes enable the visualizatio...

Fibrous aggregates in triolein are constructed from a lipophilic donor–acceptor fluorophore bearing two phenylalanine-based diamide units. Spectroscopic analyses and fluorescence imaging revealed a k...

Cardiolipin (CL), being a mitochondria-specific phospholipid, is known to play a pivotal role in cellular respiration, and its levels are intricately connected to various neurodegenerative disorders. Owing to the simplicity compared to mass spectrometry-based lipidomics, optical detection of CL is an attractive option; however, only two probes, namely TTAPE-Me and 10- nonyl acridine orange (NAO), are currently available. Herein, we report the use of an anthraquinone-based fluorophore 1 as a turn-on sensor for CL in phospholipid membranes. 1 exhibited a robust fluorescence turn-on response upon selective binding to CL, with up to 5.8- fold enhancement in dioleoyl phosphatidylcholine (DOPC) vesicles and 5.1-fold in dipalmitoyl phosphatidylcholine (DPPC) vesicles. Based on the results from anisotropy, temperature studies, and salt dependence studies, it was concluded that the formation of an electrostatic complex was the driving factor behind the observed fluorescence enhancement. Mechanistic investigations, including anisotropy, salt-dependence, and temperature studies, revealed that electrostatic complex formation underlies the observed fluorescence enhancement. Interestingly, the probe also showed a high degree of selectivity toward CL compared to other biologically relevant analytes. These findings establish compound 1 as a promising optical probe for quantitative cardiolipin detection, providing an alternative to existing fluorophores.

A donor–π–acceptor–π–donor (D–π–A–π–D) fluorophore bearing a U-shaped dipyridophenazine core forms bifurcated hydrogen bonds with neutral donors such as sulfonamides or water. This interaction modula...

Stimulated emission depletion (STED) microscopy enables super-resolution imaging of complex biological samples in 3D, in large volumes, and live. However, molecular quantification with STED has remain...

Real-time monitoring of metabolites in bacterial cultures is crucial for advancing our understanding of microbial physiology, metabolic fluxes, and dynamic responses to environmental changes. This capability enables researchers to capture transient metabolic states that are often missed in endpoint measurements. The use of engineered periplasmic binding proteins as biosensors for this real-time metabolite monitoring represents a groundbreaking approach. By leveraging the natural specificity and high affinity of PBPs for small molecules, these biosensors can be engineered to detect a wide range of metabolites with exceptional sensitivity and temporal resolution. The integration of PBP-based biosensors into microbial research not only enhances our ability to study real-time metabolism but also provides a versatile tool for optimizing industrial bioprocesses and exploring bacterial infections and complex microbial ecosystems

STED Microscopy User Group, December 11, 12 PM ET -

Via in silico docking of pyrichalasin H on G- and F-actin, the C21-OAc moiety is identified as a modifiable site tolerated for actin binding. Fluorescent [11]cytochalasan probes from pyrichalasin H a....