Wildtype One
@wildtypeone.bsky.social
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Researchers,
Misconduct can be unintentional
It could be a mistake on the bench (or in the office)
I.e., it's avoidable
We created a series around this topic
This is Part 1
Read this week's Elite Researcher's Weekly, in 3 minutes or less 👇
(...and stay tuned for Part 2 ⌛)
Misconduct can be unintentional
It could be a mistake on the bench (or in the office)
I.e., it's avoidable
We created a series around this topic
This is Part 1
Read this week's Elite Researcher's Weekly, in 3 minutes or less 👇
(...and stay tuned for Part 2 ⌛)
How to avoid "unintentional fraud"
“The important thing is to never stop questioning.” — Albert Einstein Dear Elite Researcher, Your paper can get retracted because of data concerns But you’ve never meant to fake any data It was uninte...
www.linkedin.com
November 11, 2025 at 6:38 PM
Researchers,
Misconduct can be unintentional
It could be a mistake on the bench (or in the office)
I.e., it's avoidable
We created a series around this topic
This is Part 1
Read this week's Elite Researcher's Weekly, in 3 minutes or less 👇
(...and stay tuned for Part 2 ⌛)
Misconduct can be unintentional
It could be a mistake on the bench (or in the office)
I.e., it's avoidable
We created a series around this topic
This is Part 1
Read this week's Elite Researcher's Weekly, in 3 minutes or less 👇
(...and stay tuned for Part 2 ⌛)
Choosing the wrong test can cost you statistical power.
Here's how to decide:
(a thread)
Here's how to decide:
(a thread)
November 10, 2025 at 7:17 AM
Choosing the wrong test can cost you statistical power.
Here's how to decide:
(a thread)
Here's how to decide:
(a thread)
Reposted by Wildtype One
Once again, I beg you to understand that SINGLE-CHANNEL FLUORESCENCE MICROSCOPY IMAGES DON'T HAVE A COLOR!
November 9, 2025 at 3:13 PM
Once again, I beg you to understand that SINGLE-CHANNEL FLUORESCENCE MICROSCOPY IMAGES DON'T HAVE A COLOR!
Not all lab techniques will make it to 2026
Labs evolve fast
Habits don’t
Many “standard” methods are no longer necessary
Better, more powerful options are replacing them
Here are some examples 👇
(a thread)
Labs evolve fast
Habits don’t
Many “standard” methods are no longer necessary
Better, more powerful options are replacing them
Here are some examples 👇
(a thread)
November 9, 2025 at 4:48 PM
Not all lab techniques will make it to 2026
Labs evolve fast
Habits don’t
Many “standard” methods are no longer necessary
Better, more powerful options are replacing them
Here are some examples 👇
(a thread)
Labs evolve fast
Habits don’t
Many “standard” methods are no longer necessary
Better, more powerful options are replacing them
Here are some examples 👇
(a thread)
"What should I do after the PhD?"
"Can I find a job?"
"Will I get the position I want?"
…
Here's some good news:
"Can I find a job?"
"Will I get the position I want?"
…
Here's some good news:
October 30, 2025 at 6:46 AM
"What should I do after the PhD?"
"Can I find a job?"
"Will I get the position I want?"
…
Here's some good news:
"Can I find a job?"
"Will I get the position I want?"
…
Here's some good news:
One of the most legendary papers in molecular biology was written by a postdoc...
He didn’t even mean to create it.
🧬 Quick backstory—1970
Ulrich Laemmli was a young postdoc at the MRC-LMB in Cambridge.
(a thread)
He didn’t even mean to create it.
🧬 Quick backstory—1970
Ulrich Laemmli was a young postdoc at the MRC-LMB in Cambridge.
(a thread)
October 26, 2025 at 6:25 PM
One of the most legendary papers in molecular biology was written by a postdoc...
He didn’t even mean to create it.
🧬 Quick backstory—1970
Ulrich Laemmli was a young postdoc at the MRC-LMB in Cambridge.
(a thread)
He didn’t even mean to create it.
🧬 Quick backstory—1970
Ulrich Laemmli was a young postdoc at the MRC-LMB in Cambridge.
(a thread)
Many young researchers believe the myth that isotype control is the best negative control.
Here's the truth 👇
Here's the truth 👇
October 22, 2025 at 7:01 AM
Many young researchers believe the myth that isotype control is the best negative control.
Here's the truth 👇
Here's the truth 👇
What do you think matters most when sending your draft to Nature?
Surprise: It's not your track record!
Heathcliff Dorado García (PhD and Nature Portfolio Editor) shared a few nuggets on publishing in prestigious journals ✍
Read today's Elite Researcher Weekly for more 👇
Surprise: It's not your track record!
Heathcliff Dorado García (PhD and Nature Portfolio Editor) shared a few nuggets on publishing in prestigious journals ✍
Read today's Elite Researcher Weekly for more 👇
How to publish in Nature (3-minute editor's advice)
“It doesn’t matter how beautiful your theory is… if it doesn’t agree with experiment, it’s wrong.” — Richard Feynman Dear Elite Researcher, We all would love to publish in the world’s highest-impact j...
www.linkedin.com
October 14, 2025 at 5:01 AM
What do you think matters most when sending your draft to Nature?
Surprise: It's not your track record!
Heathcliff Dorado García (PhD and Nature Portfolio Editor) shared a few nuggets on publishing in prestigious journals ✍
Read today's Elite Researcher Weekly for more 👇
Surprise: It's not your track record!
Heathcliff Dorado García (PhD and Nature Portfolio Editor) shared a few nuggets on publishing in prestigious journals ✍
Read today's Elite Researcher Weekly for more 👇
Reposted by Wildtype One
Neat trick if you polycolonal ab's suck. Incubate them with fixed cells with a KO of your protein of interest, then spin. Protocol here: www.med.upenn.edu/markslab/ass...
I was amazed how well it worked on first try (I'm sure that I can completely eliminate unspecific bands)
#WesternBlot #cellsky
I was amazed how well it worked on first try (I'm sure that I can completely eliminate unspecific bands)
#WesternBlot #cellsky
October 2, 2025 at 5:11 PM
Neat trick if you polycolonal ab's suck. Incubate them with fixed cells with a KO of your protein of interest, then spin. Protocol here: www.med.upenn.edu/markslab/ass...
I was amazed how well it worked on first try (I'm sure that I can completely eliminate unspecific bands)
#WesternBlot #cellsky
I was amazed how well it worked on first try (I'm sure that I can completely eliminate unspecific bands)
#WesternBlot #cellsky
Here's a very bad flow cytometry advice: “autofluorescence needs to be minimized”
Analogy: Imagine you're watching TV and you have static...
(a thread)
Analogy: Imagine you're watching TV and you have static...
(a thread)
October 3, 2025 at 5:20 AM
Here's a very bad flow cytometry advice: “autofluorescence needs to be minimized”
Analogy: Imagine you're watching TV and you have static...
(a thread)
Analogy: Imagine you're watching TV and you have static...
(a thread)
Albert Einstein would've hated Western blots...
(a thread)
(a thread)
September 28, 2025 at 9:14 AM
Albert Einstein would've hated Western blots...
(a thread)
(a thread)
Reposted by Wildtype One
Historians of science agree that the postdoc was invented just after the Western blot, as even the inventor wouldn't want to run one
September 23, 2025 at 2:30 PM
Historians of science agree that the postdoc was invented just after the Western blot, as even the inventor wouldn't want to run one
This quote from the famous statistician, John Tukey, had 5 warnings to biologists 🧫
(a thread)
(a thread)
September 26, 2025 at 5:18 AM
This quote from the famous statistician, John Tukey, had 5 warnings to biologists 🧫
(a thread)
(a thread)
Einstein wasn't a biologist.
But imagine he's your colleague today: What would your lab look like? How would your experiments change?
We answer that question in today's Elite Researcher Weekly (in 3 min or less) 👇
But imagine he's your colleague today: What would your lab look like? How would your experiments change?
We answer that question in today's Elite Researcher Weekly (in 3 min or less) 👇
If Einstein designed your experiments in 2025…
“If you can’t explain it to a six-year-old, you don’t understand it yourself.” — Albert Einstein Dear Elite Researcher, Einstein was NOT a wizard with his hands.
www.linkedin.com
September 24, 2025 at 5:57 AM
Einstein wasn't a biologist.
But imagine he's your colleague today: What would your lab look like? How would your experiments change?
We answer that question in today's Elite Researcher Weekly (in 3 min or less) 👇
But imagine he's your colleague today: What would your lab look like? How would your experiments change?
We answer that question in today's Elite Researcher Weekly (in 3 min or less) 👇
Nobody tells us why n=2 fails to reproduce.
So we decided to do this homework in this week's Elite Researcher Weekly.
Here are 7 reproducibility laws that great scientists obsess over (in 3 minutes or less)👇
www.linkedin.com/pulse/why-n2...
So we decided to do this homework in this week's Elite Researcher Weekly.
Here are 7 reproducibility laws that great scientists obsess over (in 3 minutes or less)👇
www.linkedin.com/pulse/why-n2...
Why n=2 doesn’t reproduce (7 laws)
“All models are wrong, but some are useful.” — George E.
www.linkedin.com
September 17, 2025 at 5:35 AM
Nobody tells us why n=2 fails to reproduce.
So we decided to do this homework in this week's Elite Researcher Weekly.
Here are 7 reproducibility laws that great scientists obsess over (in 3 minutes or less)👇
www.linkedin.com/pulse/why-n2...
So we decided to do this homework in this week's Elite Researcher Weekly.
Here are 7 reproducibility laws that great scientists obsess over (in 3 minutes or less)👇
www.linkedin.com/pulse/why-n2...
How to fix fuzzy Western blot bands
If your membrane looks like it ran in a sauna, it'd be tricky to quantify or present your data.
Let's troubleshoot💡
(a thread)
If your membrane looks like it ran in a sauna, it'd be tricky to quantify or present your data.
Let's troubleshoot💡
(a thread)
September 13, 2025 at 2:55 PM
How to fix fuzzy Western blot bands
If your membrane looks like it ran in a sauna, it'd be tricky to quantify or present your data.
Let's troubleshoot💡
(a thread)
If your membrane looks like it ran in a sauna, it'd be tricky to quantify or present your data.
Let's troubleshoot💡
(a thread)
A true story from a young researcher in Wildtype One's network...
(a thread)
(a thread)
September 10, 2025 at 3:24 PM
A true story from a young researcher in Wildtype One's network...
(a thread)
(a thread)
Unpopular opinion: The expression “Rocket science” is outdated
(a thread)
(a thread)
September 6, 2025 at 9:40 AM
Unpopular opinion: The expression “Rocket science” is outdated
(a thread)
(a thread)
Another quick tip for young researchers prepping single-cell suspensions
The common advice of “Make a single-cell suspension with enzymes” misses a critical detail.
Here’s some lesser-known advice:
(see thread)
The common advice of “Make a single-cell suspension with enzymes” misses a critical detail.
Here’s some lesser-known advice:
(see thread)
September 5, 2025 at 12:39 PM
Another quick tip for young researchers prepping single-cell suspensions
The common advice of “Make a single-cell suspension with enzymes” misses a critical detail.
Here’s some lesser-known advice:
(see thread)
The common advice of “Make a single-cell suspension with enzymes” misses a critical detail.
Here’s some lesser-known advice:
(see thread)
Struggling with flow cytometry compensation?
Single-color controls aren't the only fix.
Here’s some lesser-known advice:
(quick thread)
Single-color controls aren't the only fix.
Here’s some lesser-known advice:
(quick thread)
September 4, 2025 at 6:05 AM
Struggling with flow cytometry compensation?
Single-color controls aren't the only fix.
Here’s some lesser-known advice:
(quick thread)
Single-color controls aren't the only fix.
Here’s some lesser-known advice:
(quick thread)
Yes, there are cases where you should ignore p-values.
We made it easy for you to judge.
Read this week's issue of The Elite Researcher Weekly (3 minutes or less) 👇
www.linkedin.com/pulse/when-y...
We made it easy for you to judge.
Read this week's issue of The Elite Researcher Weekly (3 minutes or less) 👇
www.linkedin.com/pulse/when-y...
When you should ignore p-values
(and when you shouldn’t)
“You must not fool yourself — and you are the easiest person to fool.” ~ Richard Feynmann Dear Elite Researcher, Today's message is a quick lesson in statistics: P=0.
www.linkedin.com
September 1, 2025 at 9:05 AM
Yes, there are cases where you should ignore p-values.
We made it easy for you to judge.
Read this week's issue of The Elite Researcher Weekly (3 minutes or less) 👇
www.linkedin.com/pulse/when-y...
We made it easy for you to judge.
Read this week's issue of The Elite Researcher Weekly (3 minutes or less) 👇
www.linkedin.com/pulse/when-y...
A quick tip for young researchers handling dead cells in flow cytometry
The common advice of “Add a viability dye and gate them out” isn't your only option.
Here's some lesser-known advice:
(1/4)
The common advice of “Add a viability dye and gate them out” isn't your only option.
Here's some lesser-known advice:
(1/4)
August 31, 2025 at 10:35 AM
A quick tip for young researchers handling dead cells in flow cytometry
The common advice of “Add a viability dye and gate them out” isn't your only option.
Here's some lesser-known advice:
(1/4)
The common advice of “Add a viability dye and gate them out” isn't your only option.
Here's some lesser-known advice:
(1/4)
Don't fall into the trap.
Here are 5 corrections to keep in mind when interpreting RNAseq data:
(a thread)
Here are 5 corrections to keep in mind when interpreting RNAseq data:
(a thread)
August 30, 2025 at 4:42 AM
Don't fall into the trap.
Here are 5 corrections to keep in mind when interpreting RNAseq data:
(a thread)
Here are 5 corrections to keep in mind when interpreting RNAseq data:
(a thread)
Another quick warning for anyone doing live cell imaging
Live-cell imaging light dose—not just intensity—kills.
(see thread)
Live-cell imaging light dose—not just intensity—kills.
(see thread)
August 28, 2025 at 7:46 AM
Another quick warning for anyone doing live cell imaging
Live-cell imaging light dose—not just intensity—kills.
(see thread)
Live-cell imaging light dose—not just intensity—kills.
(see thread)