#Autophagy #Mechanobiology
Interesting evidence on opposite effects of ⏫matrix stiffness on Autophagy of #EndothelialCell⏬ vs #SmoothMuscleCell⏫
280~480 kPa, crosslinked poly(L-lysine)-hyaluronic acid film on coverglass
#BioactiveMat 2021
www.sciencedirect.com/science/arti...
Interesting evidence on opposite effects of ⏫matrix stiffness on Autophagy of #EndothelialCell⏬ vs #SmoothMuscleCell⏫
280~480 kPa, crosslinked poly(L-lysine)-hyaluronic acid film on coverglass
#BioactiveMat 2021
www.sciencedirect.com/science/arti...
November 12, 2025 at 7:59 PM
#Autophagy #Mechanobiology
Interesting evidence on opposite effects of ⏫matrix stiffness on Autophagy of #EndothelialCell⏬ vs #SmoothMuscleCell⏫
280~480 kPa, crosslinked poly(L-lysine)-hyaluronic acid film on coverglass
#BioactiveMat 2021
www.sciencedirect.com/science/arti...
Interesting evidence on opposite effects of ⏫matrix stiffness on Autophagy of #EndothelialCell⏬ vs #SmoothMuscleCell⏫
280~480 kPa, crosslinked poly(L-lysine)-hyaluronic acid film on coverglass
#BioactiveMat 2021
www.sciencedirect.com/science/arti...
Perturbing MEK–ERK–c-Myc bioenergetic axis in tumor #EndothelialCell suppress primary tumor growth
Evidence from an engineered tumor cell type-selective #AnthraxToxin system allowing
MEK cleavage/inactivation in selective #TumorMicroEnvironmental cell types
#PNAS 2025
www.pnas.org/doi/10.1073/...
Evidence from an engineered tumor cell type-selective #AnthraxToxin system allowing
MEK cleavage/inactivation in selective #TumorMicroEnvironmental cell types
#PNAS 2025
www.pnas.org/doi/10.1073/...
November 9, 2025 at 8:29 PM
Perturbing MEK–ERK–c-Myc bioenergetic axis in tumor #EndothelialCell suppress primary tumor growth
Evidence from an engineered tumor cell type-selective #AnthraxToxin system allowing
MEK cleavage/inactivation in selective #TumorMicroEnvironmental cell types
#PNAS 2025
www.pnas.org/doi/10.1073/...
Evidence from an engineered tumor cell type-selective #AnthraxToxin system allowing
MEK cleavage/inactivation in selective #TumorMicroEnvironmental cell types
#PNAS 2025
www.pnas.org/doi/10.1073/...
GPR153-cAMP/CREB-#YAP/TAZ or -NFκB cascades in #EndothelialCell #SmoothMuscleCell
GPR153
⏫in SMC in intima, atheroma, MI ischemic zone
⏫in EC in inflamed SkM, Brain
Myh11+ SMC Gpr153 KO 🐭
Injured artery
Slco1c1+ brain EC Gpr153 KO 🐭
EAE & Stroke
#NatComm 2025
www.nature.com/articles/s41...
GPR153
⏫in SMC in intima, atheroma, MI ischemic zone
⏫in EC in inflamed SkM, Brain
Myh11+ SMC Gpr153 KO 🐭
Injured artery
Slco1c1+ brain EC Gpr153 KO 🐭
EAE & Stroke
#NatComm 2025
www.nature.com/articles/s41...
October 25, 2025 at 11:21 AM
GPR153-cAMP/CREB-#YAP/TAZ or -NFκB cascades in #EndothelialCell #SmoothMuscleCell
GPR153
⏫in SMC in intima, atheroma, MI ischemic zone
⏫in EC in inflamed SkM, Brain
Myh11+ SMC Gpr153 KO 🐭
Injured artery
Slco1c1+ brain EC Gpr153 KO 🐭
EAE & Stroke
#NatComm 2025
www.nature.com/articles/s41...
GPR153
⏫in SMC in intima, atheroma, MI ischemic zone
⏫in EC in inflamed SkM, Brain
Myh11+ SMC Gpr153 KO 🐭
Injured artery
Slco1c1+ brain EC Gpr153 KO 🐭
EAE & Stroke
#NatComm 2025
www.nature.com/articles/s41...
BBB on a chip
#EndothelialCell #Pericyte Nanomembrane (100 nm-thick; 3 or 5 μm pore)
EC-derived laminin is insufficient to fill the pores, which can be covered by pericyte co-culture
Does vascular basement membrane have such pores?🤔
#MatTodayBio
www.sciencedirect.com/science/arti...
#EndothelialCell #Pericyte Nanomembrane (100 nm-thick; 3 or 5 μm pore)
EC-derived laminin is insufficient to fill the pores, which can be covered by pericyte co-culture
Does vascular basement membrane have such pores?🤔
#MatTodayBio
www.sciencedirect.com/science/arti...
October 22, 2025 at 7:01 PM
BBB on a chip
#EndothelialCell #Pericyte Nanomembrane (100 nm-thick; 3 or 5 μm pore)
EC-derived laminin is insufficient to fill the pores, which can be covered by pericyte co-culture
Does vascular basement membrane have such pores?🤔
#MatTodayBio
www.sciencedirect.com/science/arti...
#EndothelialCell #Pericyte Nanomembrane (100 nm-thick; 3 or 5 μm pore)
EC-derived laminin is insufficient to fill the pores, which can be covered by pericyte co-culture
Does vascular basement membrane have such pores?🤔
#MatTodayBio
www.sciencedirect.com/science/arti...
INduced Pdgfrb+ #Pericyte ITGA8 KO🐭
Baseline by 40 d
⏬microvascular pericytic coverage
⏬ #EndothelialCell #TightJunction
⏬ #BloodBrainBarrier
⏬EC-Pericyte N-cad junction
⏫Degenerated neurons
⏫Neurobehavioral anomalies
#AdvSci 2025
advanced.onlinelibrary.wiley.com/doi/10.1002/...
Baseline by 40 d
⏬microvascular pericytic coverage
⏬ #EndothelialCell #TightJunction
⏬ #BloodBrainBarrier
⏬EC-Pericyte N-cad junction
⏫Degenerated neurons
⏫Neurobehavioral anomalies
#AdvSci 2025
advanced.onlinelibrary.wiley.com/doi/10.1002/...
October 16, 2025 at 7:17 PM
INduced Pdgfrb+ #Pericyte ITGA8 KO🐭
Baseline by 40 d
⏬microvascular pericytic coverage
⏬ #EndothelialCell #TightJunction
⏬ #BloodBrainBarrier
⏬EC-Pericyte N-cad junction
⏫Degenerated neurons
⏫Neurobehavioral anomalies
#AdvSci 2025
advanced.onlinelibrary.wiley.com/doi/10.1002/...
Baseline by 40 d
⏬microvascular pericytic coverage
⏬ #EndothelialCell #TightJunction
⏬ #BloodBrainBarrier
⏬EC-Pericyte N-cad junction
⏫Degenerated neurons
⏫Neurobehavioral anomalies
#AdvSci 2025
advanced.onlinelibrary.wiley.com/doi/10.1002/...
#Atherosclerosis Epitranscriptomics
Cell type-specific dysregulation of m6A machinery in atherogenic phenotypes
#EndothelialCell ALKBH5
#SmoothMuscleCell WTAP
Mφ METTL3 YTHDF2
Re-analysis of scRNAseq Wirka's Nat Med 2019
pmc.ncbi.nlm.nih.gov/articles/PMC...
#THNO 2025
www.thno.org/v15p4785.htm
Cell type-specific dysregulation of m6A machinery in atherogenic phenotypes
#EndothelialCell ALKBH5
#SmoothMuscleCell WTAP
Mφ METTL3 YTHDF2
Re-analysis of scRNAseq Wirka's Nat Med 2019
pmc.ncbi.nlm.nih.gov/articles/PMC...
#THNO 2025
www.thno.org/v15p4785.htm
October 14, 2025 at 7:31 PM
#Atherosclerosis Epitranscriptomics
Cell type-specific dysregulation of m6A machinery in atherogenic phenotypes
#EndothelialCell ALKBH5
#SmoothMuscleCell WTAP
Mφ METTL3 YTHDF2
Re-analysis of scRNAseq Wirka's Nat Med 2019
pmc.ncbi.nlm.nih.gov/articles/PMC...
#THNO 2025
www.thno.org/v15p4785.htm
Cell type-specific dysregulation of m6A machinery in atherogenic phenotypes
#EndothelialCell ALKBH5
#SmoothMuscleCell WTAP
Mφ METTL3 YTHDF2
Re-analysis of scRNAseq Wirka's Nat Med 2019
pmc.ncbi.nlm.nih.gov/articles/PMC...
#THNO 2025
www.thno.org/v15p4785.htm
#WernerSyndrome hiPSC engineered vessel
Aligned #SmoothMuscleCell in Collagen hydrogel➡️
Roll-up as helical tube
+PLA mold
+Luminal #EndothelialCell
#SpatialTranscriptomics
#Metabolomics
Vessels with Werner EC are Hypercontractile even with healthy SMC
bioRxiv 2025
www.biorxiv.org/content/10.1...
Aligned #SmoothMuscleCell in Collagen hydrogel➡️
Roll-up as helical tube
+PLA mold
+Luminal #EndothelialCell
#SpatialTranscriptomics
#Metabolomics
Vessels with Werner EC are Hypercontractile even with healthy SMC
bioRxiv 2025
www.biorxiv.org/content/10.1...
October 4, 2025 at 7:06 PM
#WernerSyndrome hiPSC engineered vessel
Aligned #SmoothMuscleCell in Collagen hydrogel➡️
Roll-up as helical tube
+PLA mold
+Luminal #EndothelialCell
#SpatialTranscriptomics
#Metabolomics
Vessels with Werner EC are Hypercontractile even with healthy SMC
bioRxiv 2025
www.biorxiv.org/content/10.1...
Aligned #SmoothMuscleCell in Collagen hydrogel➡️
Roll-up as helical tube
+PLA mold
+Luminal #EndothelialCell
#SpatialTranscriptomics
#Metabolomics
Vessels with Werner EC are Hypercontractile even with healthy SMC
bioRxiv 2025
www.biorxiv.org/content/10.1...
#TissueEngineeredBloodVessel
Decellularized 👤umbilical cord artery
Seeded: #hiPSC #EndothelialCell
Bioreactor trained: pulsatile unidirec flow 15➡️5 dyn/cm2
Rat #InferiorVenaCava graft▶️
0️⃣Thrombosis
Complete repop by host EC 2 wk SMC 2 mo
#CellStemCell 2025
www.sciencedirect.com/science/arti...
Decellularized 👤umbilical cord artery
Seeded: #hiPSC #EndothelialCell
Bioreactor trained: pulsatile unidirec flow 15➡️5 dyn/cm2
Rat #InferiorVenaCava graft▶️
0️⃣Thrombosis
Complete repop by host EC 2 wk SMC 2 mo
#CellStemCell 2025
www.sciencedirect.com/science/arti...
October 1, 2025 at 7:56 PM
#TissueEngineeredBloodVessel
Decellularized 👤umbilical cord artery
Seeded: #hiPSC #EndothelialCell
Bioreactor trained: pulsatile unidirec flow 15➡️5 dyn/cm2
Rat #InferiorVenaCava graft▶️
0️⃣Thrombosis
Complete repop by host EC 2 wk SMC 2 mo
#CellStemCell 2025
www.sciencedirect.com/science/arti...
Decellularized 👤umbilical cord artery
Seeded: #hiPSC #EndothelialCell
Bioreactor trained: pulsatile unidirec flow 15➡️5 dyn/cm2
Rat #InferiorVenaCava graft▶️
0️⃣Thrombosis
Complete repop by host EC 2 wk SMC 2 mo
#CellStemCell 2025
www.sciencedirect.com/science/arti...
A 3D fibrin hydrogel supportive neurovascular cell niche
17 kPa ECM stiffness
👤🧠 #EndothelialCell #Pericyte #Astrocyte
⏫Quiescence, invasiveness, temozolomide resistance of patient-derived #Glioblastoma stem cell lines or tumorsphere
#AdvSci 2025
advanced.onlinelibrary.wiley.com/doi/10.1002/...
17 kPa ECM stiffness
👤🧠 #EndothelialCell #Pericyte #Astrocyte
⏫Quiescence, invasiveness, temozolomide resistance of patient-derived #Glioblastoma stem cell lines or tumorsphere
#AdvSci 2025
advanced.onlinelibrary.wiley.com/doi/10.1002/...
October 1, 2025 at 11:36 AM
A 3D fibrin hydrogel supportive neurovascular cell niche
17 kPa ECM stiffness
👤🧠 #EndothelialCell #Pericyte #Astrocyte
⏫Quiescence, invasiveness, temozolomide resistance of patient-derived #Glioblastoma stem cell lines or tumorsphere
#AdvSci 2025
advanced.onlinelibrary.wiley.com/doi/10.1002/...
17 kPa ECM stiffness
👤🧠 #EndothelialCell #Pericyte #Astrocyte
⏫Quiescence, invasiveness, temozolomide resistance of patient-derived #Glioblastoma stem cell lines or tumorsphere
#AdvSci 2025
advanced.onlinelibrary.wiley.com/doi/10.1002/...
#CaseReport
Heterozygous ERG (#EndothelialCell lineage marker) p.Arg212Leu➡️
Abdominal aortic & multi-arterial Aneurysm 52 y/o proband
Cervical artery dissection➡️ischemic stroke at 18 y/o son of proband
#FrontCVM 2025
www.frontiersin.org/journals/car...
Heterozygous ERG (#EndothelialCell lineage marker) p.Arg212Leu➡️
Abdominal aortic & multi-arterial Aneurysm 52 y/o proband
Cervical artery dissection➡️ischemic stroke at 18 y/o son of proband
#FrontCVM 2025
www.frontiersin.org/journals/car...
September 27, 2025 at 7:09 PM
#CaseReport
Heterozygous ERG (#EndothelialCell lineage marker) p.Arg212Leu➡️
Abdominal aortic & multi-arterial Aneurysm 52 y/o proband
Cervical artery dissection➡️ischemic stroke at 18 y/o son of proband
#FrontCVM 2025
www.frontiersin.org/journals/car...
Heterozygous ERG (#EndothelialCell lineage marker) p.Arg212Leu➡️
Abdominal aortic & multi-arterial Aneurysm 52 y/o proband
Cervical artery dissection➡️ischemic stroke at 18 y/o son of proband
#FrontCVM 2025
www.frontiersin.org/journals/car...
#DryAMD #AgerelatedMacularDegeneration
Rat focal #ChoroidAtropy◀️NaIO3 into suprachoroidal space
⏬Choroid thickness
⏬vessel density/junctions
partial rescued by #hiPSC-#EndothelialCell transplant 7 dpi
👉making 30~40% of choroid vessels
#AdvSci 2025
advanced.onlinelibrary.wiley.com/doi/10.1002/...
Rat focal #ChoroidAtropy◀️NaIO3 into suprachoroidal space
⏬Choroid thickness
⏬vessel density/junctions
partial rescued by #hiPSC-#EndothelialCell transplant 7 dpi
👉making 30~40% of choroid vessels
#AdvSci 2025
advanced.onlinelibrary.wiley.com/doi/10.1002/...
September 24, 2025 at 12:24 PM
#DryAMD #AgerelatedMacularDegeneration
Rat focal #ChoroidAtropy◀️NaIO3 into suprachoroidal space
⏬Choroid thickness
⏬vessel density/junctions
partial rescued by #hiPSC-#EndothelialCell transplant 7 dpi
👉making 30~40% of choroid vessels
#AdvSci 2025
advanced.onlinelibrary.wiley.com/doi/10.1002/...
Rat focal #ChoroidAtropy◀️NaIO3 into suprachoroidal space
⏬Choroid thickness
⏬vessel density/junctions
partial rescued by #hiPSC-#EndothelialCell transplant 7 dpi
👉making 30~40% of choroid vessels
#AdvSci 2025
advanced.onlinelibrary.wiley.com/doi/10.1002/...
#Senescence #Mechanobiology
Senescent #EndothelialCell HUVEC (+H2O2 or Doxorubicin) have ⬆️ #Viscoelasticity
(Microfluidic cytometric measurement of suspended whole-cell deformation)
⏫F-actin, Calnexin ER, Lamin A Nuclear envelope
#CellRepPhysSci 2025
www.sciencedirect.com/science/arti...
Senescent #EndothelialCell HUVEC (+H2O2 or Doxorubicin) have ⬆️ #Viscoelasticity
(Microfluidic cytometric measurement of suspended whole-cell deformation)
⏫F-actin, Calnexin ER, Lamin A Nuclear envelope
#CellRepPhysSci 2025
www.sciencedirect.com/science/arti...
September 20, 2025 at 7:06 PM
#Senescence #Mechanobiology
Senescent #EndothelialCell HUVEC (+H2O2 or Doxorubicin) have ⬆️ #Viscoelasticity
(Microfluidic cytometric measurement of suspended whole-cell deformation)
⏫F-actin, Calnexin ER, Lamin A Nuclear envelope
#CellRepPhysSci 2025
www.sciencedirect.com/science/arti...
Senescent #EndothelialCell HUVEC (+H2O2 or Doxorubicin) have ⬆️ #Viscoelasticity
(Microfluidic cytometric measurement of suspended whole-cell deformation)
⏫F-actin, Calnexin ER, Lamin A Nuclear envelope
#CellRepPhysSci 2025
www.sciencedirect.com/science/arti...
#Stroke #Angiogenesis
Post-stroke (7 dpi) KO of #EndothelialCell HDAC2🐭
👉⏫Preserved Cortical volume by 8 wk
👉⏫Neuron survival
👉⏫Angiogensis
👉⏫#BloodBrainBarrier (3 kD dextran)
Kumar Peeyush lab bioRxiv 2025
www.biorxiv.org/content/10.1...
Post-stroke (7 dpi) KO of #EndothelialCell HDAC2🐭
👉⏫Preserved Cortical volume by 8 wk
👉⏫Neuron survival
👉⏫Angiogensis
👉⏫#BloodBrainBarrier (3 kD dextran)
Kumar Peeyush lab bioRxiv 2025
www.biorxiv.org/content/10.1...
September 17, 2025 at 10:18 AM
#Stroke #Angiogenesis
Post-stroke (7 dpi) KO of #EndothelialCell HDAC2🐭
👉⏫Preserved Cortical volume by 8 wk
👉⏫Neuron survival
👉⏫Angiogensis
👉⏫#BloodBrainBarrier (3 kD dextran)
Kumar Peeyush lab bioRxiv 2025
www.biorxiv.org/content/10.1...
Post-stroke (7 dpi) KO of #EndothelialCell HDAC2🐭
👉⏫Preserved Cortical volume by 8 wk
👉⏫Neuron survival
👉⏫Angiogensis
👉⏫#BloodBrainBarrier (3 kD dextran)
Kumar Peeyush lab bioRxiv 2025
www.biorxiv.org/content/10.1...
AAV-BI30, an endotheliotropic #AdenoAssociatedVirus for transducing vascular tree in #CentralNervousSystem Brain/Retina/Spinal Cord
👉>AAV-BR1 esp. in artery/vein
👉Also transduce #EndothelialCell in #Aorta/Liver/Lung/Kidney
@natcardiovascres.nature.com 2022
pmc.ncbi.nlm.nih.gov/articles/PMC...
👉>AAV-BR1 esp. in artery/vein
👉Also transduce #EndothelialCell in #Aorta/Liver/Lung/Kidney
@natcardiovascres.nature.com 2022
pmc.ncbi.nlm.nih.gov/articles/PMC...
September 14, 2025 at 7:10 PM
AAV-BI30, an endotheliotropic #AdenoAssociatedVirus for transducing vascular tree in #CentralNervousSystem Brain/Retina/Spinal Cord
👉>AAV-BR1 esp. in artery/vein
👉Also transduce #EndothelialCell in #Aorta/Liver/Lung/Kidney
@natcardiovascres.nature.com 2022
pmc.ncbi.nlm.nih.gov/articles/PMC...
👉>AAV-BR1 esp. in artery/vein
👉Also transduce #EndothelialCell in #Aorta/Liver/Lung/Kidney
@natcardiovascres.nature.com 2022
pmc.ncbi.nlm.nih.gov/articles/PMC...
Stroke Atlas
anratherlab.shinyapps.io/strokevis/
Single-cell transcriptomics 🐭Brain+Blood
2 & 14 days post-stroke
♀️♂️
Young(2~3 mo) Aged (17~20 mo)
9 #EndothelialCell clusters
IGF1 signals
#Aging
⏫interferon signals in post-stroke🧠EC (+immune cell)
#NatImmunol 2024
www.nature.com/articles/s41...
anratherlab.shinyapps.io/strokevis/
Single-cell transcriptomics 🐭Brain+Blood
2 & 14 days post-stroke
♀️♂️
Young(2~3 mo) Aged (17~20 mo)
9 #EndothelialCell clusters
IGF1 signals
#Aging
⏫interferon signals in post-stroke🧠EC (+immune cell)
#NatImmunol 2024
www.nature.com/articles/s41...
September 13, 2025 at 7:08 PM
Stroke Atlas
anratherlab.shinyapps.io/strokevis/
Single-cell transcriptomics 🐭Brain+Blood
2 & 14 days post-stroke
♀️♂️
Young(2~3 mo) Aged (17~20 mo)
9 #EndothelialCell clusters
IGF1 signals
#Aging
⏫interferon signals in post-stroke🧠EC (+immune cell)
#NatImmunol 2024
www.nature.com/articles/s41...
anratherlab.shinyapps.io/strokevis/
Single-cell transcriptomics 🐭Brain+Blood
2 & 14 days post-stroke
♀️♂️
Young(2~3 mo) Aged (17~20 mo)
9 #EndothelialCell clusters
IGF1 signals
#Aging
⏫interferon signals in post-stroke🧠EC (+immune cell)
#NatImmunol 2024
www.nature.com/articles/s41...
#Malaria #BloodBrainBarrier
ETS (ETV2/ERG/FLI)-enhanced #hiPSC-#EndothelialCell models BBB destab. by P. falciparum (malaria parasite)-infected RBC
👉⏬Epithelial features
👉Similar Barrier in 3D microvessel
👉⏫Cytoadhesion of P. falciparum-infected RBCs
bioRxiv 2025
www.biorxiv.org/content/10.1...
ETS (ETV2/ERG/FLI)-enhanced #hiPSC-#EndothelialCell models BBB destab. by P. falciparum (malaria parasite)-infected RBC
👉⏬Epithelial features
👉Similar Barrier in 3D microvessel
👉⏫Cytoadhesion of P. falciparum-infected RBCs
bioRxiv 2025
www.biorxiv.org/content/10.1...
September 13, 2025 at 11:10 AM
#Malaria #BloodBrainBarrier
ETS (ETV2/ERG/FLI)-enhanced #hiPSC-#EndothelialCell models BBB destab. by P. falciparum (malaria parasite)-infected RBC
👉⏬Epithelial features
👉Similar Barrier in 3D microvessel
👉⏫Cytoadhesion of P. falciparum-infected RBCs
bioRxiv 2025
www.biorxiv.org/content/10.1...
ETS (ETV2/ERG/FLI)-enhanced #hiPSC-#EndothelialCell models BBB destab. by P. falciparum (malaria parasite)-infected RBC
👉⏬Epithelial features
👉Similar Barrier in 3D microvessel
👉⏫Cytoadhesion of P. falciparum-infected RBCs
bioRxiv 2025
www.biorxiv.org/content/10.1...
Single-cell transcriptomics #SuperficialTemporalArtery #MoyamoyaDisease
2 Case + 3 Ctrl (epilepsy)
SMC1: contractile OXPHOS
SMC2: ribosome
Proliferative signaling
✅#NaturalKiller MIF➡️ SMC Fibroblast
#EndothelialCell
#JAHA 2025 @ahajournals.bsky.social
www.ahajournals.org/doi/10.1161/...
2 Case + 3 Ctrl (epilepsy)
SMC1: contractile OXPHOS
SMC2: ribosome
Proliferative signaling
✅#NaturalKiller MIF➡️ SMC Fibroblast
#EndothelialCell
#JAHA 2025 @ahajournals.bsky.social
www.ahajournals.org/doi/10.1161/...
September 12, 2025 at 7:21 PM
Single-cell transcriptomics #SuperficialTemporalArtery #MoyamoyaDisease
2 Case + 3 Ctrl (epilepsy)
SMC1: contractile OXPHOS
SMC2: ribosome
Proliferative signaling
✅#NaturalKiller MIF➡️ SMC Fibroblast
#EndothelialCell
#JAHA 2025 @ahajournals.bsky.social
www.ahajournals.org/doi/10.1161/...
2 Case + 3 Ctrl (epilepsy)
SMC1: contractile OXPHOS
SMC2: ribosome
Proliferative signaling
✅#NaturalKiller MIF➡️ SMC Fibroblast
#EndothelialCell
#JAHA 2025 @ahajournals.bsky.social
www.ahajournals.org/doi/10.1161/...
#HistoneLactylation #Angiogenesis
VEGF-A➡️
⏫#EndothelialCell #Glycolysis #Lactate➡️
⏫H3K9la➡️
>50% CUT&Tag peaks within 3 kb promoters
Covering prolif/motility/junction/hypoxia
Oxygen-induced retinopathy ⬇️ by glycolytic inhibitors
#GenomeBiol 2024
genomebiology.biomedcentral.com/articles/10....
VEGF-A➡️
⏫#EndothelialCell #Glycolysis #Lactate➡️
⏫H3K9la➡️
>50% CUT&Tag peaks within 3 kb promoters
Covering prolif/motility/junction/hypoxia
Oxygen-induced retinopathy ⬇️ by glycolytic inhibitors
#GenomeBiol 2024
genomebiology.biomedcentral.com/articles/10....
September 11, 2025 at 7:43 PM
#HistoneLactylation #Angiogenesis
VEGF-A➡️
⏫#EndothelialCell #Glycolysis #Lactate➡️
⏫H3K9la➡️
>50% CUT&Tag peaks within 3 kb promoters
Covering prolif/motility/junction/hypoxia
Oxygen-induced retinopathy ⬇️ by glycolytic inhibitors
#GenomeBiol 2024
genomebiology.biomedcentral.com/articles/10....
VEGF-A➡️
⏫#EndothelialCell #Glycolysis #Lactate➡️
⏫H3K9la➡️
>50% CUT&Tag peaks within 3 kb promoters
Covering prolif/motility/junction/hypoxia
Oxygen-induced retinopathy ⬇️ by glycolytic inhibitors
#GenomeBiol 2024
genomebiology.biomedcentral.com/articles/10....
#Malaria #BloodBrainBarrier
P. falciparum (most severe human malaria parasite strain)-infected #RedBloodCell➡️
Local disruption of #EndothelialCell barrier in 3D microfluidic BBB (EC Pericyte Astrocyte)
⏫Interferon-JAK-STAT1 signals in all 3 cell types
#NatCommun 2025
www.nature.com/articles/s41...
P. falciparum (most severe human malaria parasite strain)-infected #RedBloodCell➡️
Local disruption of #EndothelialCell barrier in 3D microfluidic BBB (EC Pericyte Astrocyte)
⏫Interferon-JAK-STAT1 signals in all 3 cell types
#NatCommun 2025
www.nature.com/articles/s41...
September 9, 2025 at 7:03 PM
#Malaria #BloodBrainBarrier
P. falciparum (most severe human malaria parasite strain)-infected #RedBloodCell➡️
Local disruption of #EndothelialCell barrier in 3D microfluidic BBB (EC Pericyte Astrocyte)
⏫Interferon-JAK-STAT1 signals in all 3 cell types
#NatCommun 2025
www.nature.com/articles/s41...
P. falciparum (most severe human malaria parasite strain)-infected #RedBloodCell➡️
Local disruption of #EndothelialCell barrier in 3D microfluidic BBB (EC Pericyte Astrocyte)
⏫Interferon-JAK-STAT1 signals in all 3 cell types
#NatCommun 2025
www.nature.com/articles/s41...
Endothelin-1➡️ #EndothelialCell to #DorsalRootGanglion signaling in #VascularPain
✅Mechanical ❎Heat ❎Cold
1⃣🐭Limb ischemia
2⃣🐭Optogenetic EC ChR2 nociception
3⃣🐭Chemogenetic EC hM3Dq nociception
4⃣🧍♂️Postop tourniquet pain⬇️by Bosentan ETA/Bi
#AdvSci 2025
advanced.onlinelibrary.wiley.com/doi/10.1002/...
✅Mechanical ❎Heat ❎Cold
1⃣🐭Limb ischemia
2⃣🐭Optogenetic EC ChR2 nociception
3⃣🐭Chemogenetic EC hM3Dq nociception
4⃣🧍♂️Postop tourniquet pain⬇️by Bosentan ETA/Bi
#AdvSci 2025
advanced.onlinelibrary.wiley.com/doi/10.1002/...
September 5, 2025 at 11:15 AM
Endothelin-1➡️ #EndothelialCell to #DorsalRootGanglion signaling in #VascularPain
✅Mechanical ❎Heat ❎Cold
1⃣🐭Limb ischemia
2⃣🐭Optogenetic EC ChR2 nociception
3⃣🐭Chemogenetic EC hM3Dq nociception
4⃣🧍♂️Postop tourniquet pain⬇️by Bosentan ETA/Bi
#AdvSci 2025
advanced.onlinelibrary.wiley.com/doi/10.1002/...
✅Mechanical ❎Heat ❎Cold
1⃣🐭Limb ischemia
2⃣🐭Optogenetic EC ChR2 nociception
3⃣🐭Chemogenetic EC hM3Dq nociception
4⃣🧍♂️Postop tourniquet pain⬇️by Bosentan ETA/Bi
#AdvSci 2025
advanced.onlinelibrary.wiley.com/doi/10.1002/...
Fabrication of #MicrovesselOnAChip
#EndothelialCell HUVEC
3D printing injection reservoirs
Needle-based collagen channel φ200 μm
Sprouting
100 Pa intraluminal pressure
4 & 70 kD FITC-dextran for permeability
A. Bancaud & Y. Matsunaga labs #STARProtoc 2024
www.sciencedirect.com/science/arti...
#EndothelialCell HUVEC
3D printing injection reservoirs
Needle-based collagen channel φ200 μm
Sprouting
100 Pa intraluminal pressure
4 & 70 kD FITC-dextran for permeability
A. Bancaud & Y. Matsunaga labs #STARProtoc 2024
www.sciencedirect.com/science/arti...
September 3, 2025 at 11:29 AM
Fabrication of #MicrovesselOnAChip
#EndothelialCell HUVEC
3D printing injection reservoirs
Needle-based collagen channel φ200 μm
Sprouting
100 Pa intraluminal pressure
4 & 70 kD FITC-dextran for permeability
A. Bancaud & Y. Matsunaga labs #STARProtoc 2024
www.sciencedirect.com/science/arti...
#EndothelialCell HUVEC
3D printing injection reservoirs
Needle-based collagen channel φ200 μm
Sprouting
100 Pa intraluminal pressure
4 & 70 kD FITC-dextran for permeability
A. Bancaud & Y. Matsunaga labs #STARProtoc 2024
www.sciencedirect.com/science/arti...
triggers inflammation throughout the body by activating immune & #endothelialcells.C5a also promotes the release of pro-inflammatory cytokines like IL-6.
- Vascular Injury: The formation and insertion of TCC into #endothelialcell membranes directly cause cellular injury and death,
- Vascular Injury: The formation and insertion of TCC into #endothelialcell membranes directly cause cellular injury and death,
September 2, 2025 at 3:35 PM
triggers inflammation throughout the body by activating immune & #endothelialcells.C5a also promotes the release of pro-inflammatory cytokines like IL-6.
- Vascular Injury: The formation and insertion of TCC into #endothelialcell membranes directly cause cellular injury and death,
- Vascular Injury: The formation and insertion of TCC into #endothelialcell membranes directly cause cellular injury and death,
Lineage Origins of mouse renal #LymphaticEndothelialCell
85% by Tie2+ Venous #endothelialCell
15% by Osr1+ Intermediate Mesoderm😎
Lymphvasculogenesis (60% Tie2+ vs 40% Osr1+)
9 different Cre🐭🤠
@daniyaljafree.bsky.social bioRxiv 2025
www.biorxiv.org/content/10.1...
85% by Tie2+ Venous #endothelialCell
15% by Osr1+ Intermediate Mesoderm😎
Lymphvasculogenesis (60% Tie2+ vs 40% Osr1+)
9 different Cre🐭🤠
@daniyaljafree.bsky.social bioRxiv 2025
www.biorxiv.org/content/10.1...
Genetic lineage tracing identifies intermediate mesoderm as a novel contributor to mammalian kidney lymphatics
The lymphatic vasculature is essential for fluid homeostasis, immune regulation and possesses diverse organ-specific functions. During development, lymphatic endothelial cells (LEC) arise from multipl...
www.biorxiv.org
August 7, 2025 at 5:13 PM
Lineage Origins of mouse renal #LymphaticEndothelialCell
85% by Tie2+ Venous #endothelialCell
15% by Osr1+ Intermediate Mesoderm😎
Lymphvasculogenesis (60% Tie2+ vs 40% Osr1+)
9 different Cre🐭🤠
@daniyaljafree.bsky.social bioRxiv 2025
www.biorxiv.org/content/10.1...
85% by Tie2+ Venous #endothelialCell
15% by Osr1+ Intermediate Mesoderm😎
Lymphvasculogenesis (60% Tie2+ vs 40% Osr1+)
9 different Cre🐭🤠
@daniyaljafree.bsky.social bioRxiv 2025
www.biorxiv.org/content/10.1...
Tie2+ #EndothelialCell- or Col1A2+ fibroblast-derived C-type Natriuretic Peptide protects against #AorticAneurysm in Apoe-/- mice + AngII + AAV-PCSK9 + #WesternDiet
#SmoothMuscleCell & Mφ are responsive to exogenous CNP
#ATVB 2025 @ahajournals.bsky.social
www.ahajournals.org/doi/10.1161/...
#SmoothMuscleCell & Mφ are responsive to exogenous CNP
#ATVB 2025 @ahajournals.bsky.social
www.ahajournals.org/doi/10.1161/...
August 7, 2025 at 10:06 AM
Tie2+ #EndothelialCell- or Col1A2+ fibroblast-derived C-type Natriuretic Peptide protects against #AorticAneurysm in Apoe-/- mice + AngII + AAV-PCSK9 + #WesternDiet
#SmoothMuscleCell & Mφ are responsive to exogenous CNP
#ATVB 2025 @ahajournals.bsky.social
www.ahajournals.org/doi/10.1161/...
#SmoothMuscleCell & Mφ are responsive to exogenous CNP
#ATVB 2025 @ahajournals.bsky.social
www.ahajournals.org/doi/10.1161/...
Anti-isoDGR immunotherapy stabilizes Aged 🐭 #BloodBrainBarrier
#ExtraCellularMatrix Protein Deamidation
Asn-Gly-Arg/NRG➡️isoaspartate-Gly-Arg/isoDGR
⏫in #Aging
isoDGR on #EndothelialCell in vitro
👉Actin cytoskeletal alterations
👉Inflammation
👉Senescence
bioRxiv 2025
www.biorxiv.org/content/10.1...
#ExtraCellularMatrix Protein Deamidation
Asn-Gly-Arg/NRG➡️isoaspartate-Gly-Arg/isoDGR
⏫in #Aging
isoDGR on #EndothelialCell in vitro
👉Actin cytoskeletal alterations
👉Inflammation
👉Senescence
bioRxiv 2025
www.biorxiv.org/content/10.1...
August 6, 2025 at 7:07 PM
Anti-isoDGR immunotherapy stabilizes Aged 🐭 #BloodBrainBarrier
#ExtraCellularMatrix Protein Deamidation
Asn-Gly-Arg/NRG➡️isoaspartate-Gly-Arg/isoDGR
⏫in #Aging
isoDGR on #EndothelialCell in vitro
👉Actin cytoskeletal alterations
👉Inflammation
👉Senescence
bioRxiv 2025
www.biorxiv.org/content/10.1...
#ExtraCellularMatrix Protein Deamidation
Asn-Gly-Arg/NRG➡️isoaspartate-Gly-Arg/isoDGR
⏫in #Aging
isoDGR on #EndothelialCell in vitro
👉Actin cytoskeletal alterations
👉Inflammation
👉Senescence
bioRxiv 2025
www.biorxiv.org/content/10.1...