Urinary tract infections (UTIs) are among the most common infectious diseases, causing over 400 million cases and 260,000 deaths annually. Women are disproportionately affected, with ∼50% experiencing...

About the Conference Codon usage bias—the preference for certain synonymous codons—is a key factor in genome regulation. Codon usage and synonymous codon mutations have been shown to influence gene ex...

Bacteria defend against hostile environments through a variety of molecular mechanisms, including ribosome hibernation. Previously, bacteria were shown to initiate ribosome hibernation by activating p...

RNAs move through the extracellular space to transmit information between cells, including mammalian neurons, yet how specific RNAs are channeled into these extracellular routes is unknown. Using geno...

Intrinsic resistance pathways in E. coli can be exploited to enhance antibiotic sensitivity and limit resistance evolution. This study shows that genetic inhibition of efflux pumps and cell envelope b...

Introducing novel antibiotics is essential to counter the spread of drug-resistant pathogens. Here, we report the discovery of 4′-thiothymidine (4′sT), a nucleoside antibiotic from the nematode symbiont Photorhabdus asymbiotica, identified through activation of a silent biosynthetic gene cluster. 4′sT features an unusual 4′-thiosugar moiety. We identified its biosynthetic gene cluster, including a radical SAM enzyme presumably involved in sulfur incorporation. 4′sT exhibits strain-selective activity that is governed by differences in thymidine metabolism rather than variations in the molecular target. These findings expand our knowledge of antibiotics with unusual selective activity.

Author summary This study charts the first genome-wide map of transfer RNA (tRNA) modifications in the cholera pathogen, Vibrio cholerae, revealing how chemical marks on tRNAs shape translation and st...

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Proteomics analysis across human tissues from the GTeX resource reveals insight into tissue-specific pathways and phenotypes arising from genetic diseases.

Les aminosides sont des antibiotiques efficaces contre de très nombreuses bactéries telles que Escherichia coli, Pseudomonas aeruginosa ou Staphylococcus aureus. Mais jusqu’à présent, personne ne sava...

Nature Communications - Mutations in elongation factor G protect bacteria from aminoglycoside antibiotics through unknown mechanisms. Here, the authors show that the mutations selectively slow the...

Abstract. Natural chromosomal transformation (NCT) in Bacillus subtilis requires RecA and its accessory proteins including RecX and RecD2. Inactivation of

Abstract. Toxins from toxin–antitoxin (TA) systems, widely distributed genetic elements in prokaryotic genomes, are traditionally viewed as growth inhibito

Pan-Mod-seq enables systematic, multi-modification mapping of rRNA across life. Applying it to diverse organisms reveals that hyperthermophiles dynamically install stabilizing modifications to support...

Chemical modifications of rRNA, tRNA, and mRNA play key roles in protein synthesis by affecting the structure of these RNAs, by modulating decoding, and by influencing ribosomal efficiency. Recent adv...

This study introduces single-cell transcription factor (TF) sequencing, a single-cell barcoded and doxycycline-inducible TF overexpression approach that reveals dose-sensitive functional classes of TF...

Scientific Reports - Genomic characterization of Microbacterium meiriae sp. nov., a novel bacterium isolated from the International Space Station

Ribosome hibernation preserves translation machinery during stress, yet its mechanisms in Archaea remain poorly defined. Here we identify Hib, a previously unrecognized family of archaeal hibernation ...