Zak Baker
@zakbaker.bsky.social
Postdoc in the Pagliarini Lab at WashU. Focusing mitochondria, lipids and everything mass spec.
Reposted by Zak Baker
Very excited to share the published version of our #uridine #CRISPR screen in naturemetabolism.bsky.social! 🎉
Led by Abigail Strefeler with @pagliarini-lab.bsky.social @zakbaker.bsky.social and team!
#NUDT5 #DMQ @dib-unil.bsky.social @fbm-unil.bsky.social
🔗 www.nature.com/articles/s42...
🧵1/17
Led by Abigail Strefeler with @pagliarini-lab.bsky.social @zakbaker.bsky.social and team!
#NUDT5 #DMQ @dib-unil.bsky.social @fbm-unil.bsky.social
🔗 www.nature.com/articles/s42...
🧵1/17
Uridine-sensitized screening identifies demethoxy-coenzyme Q and NUDT5 as regulators of nucleotide synthesis - Nature Metabolism
A uridine-sensitized CRISPR-Cas9 screening identifies demethoxy-CoQ as an alternative electron acceptor in the absence of CoQ, and NUDT5 as a regulator of de novo pyrimidine synthesis via its interact...
www.nature.com
November 13, 2025 at 10:19 AM
Very excited to share the published version of our #uridine #CRISPR screen in naturemetabolism.bsky.social! 🎉
Led by Abigail Strefeler with @pagliarini-lab.bsky.social @zakbaker.bsky.social and team!
#NUDT5 #DMQ @dib-unil.bsky.social @fbm-unil.bsky.social
🔗 www.nature.com/articles/s42...
🧵1/17
Led by Abigail Strefeler with @pagliarini-lab.bsky.social @zakbaker.bsky.social and team!
#NUDT5 #DMQ @dib-unil.bsky.social @fbm-unil.bsky.social
🔗 www.nature.com/articles/s42...
🧵1/17
Reposted by Zak Baker
AND IT'S DONE! A 🧵 on our recent article now out in @natsmb.nature.com! Co-led with @abbybartlett.bsky.social, Pagliarini Lab, @judisimcox.bsky.social, we find ACAD10/11 are NOT like other acyl-CoA dehydrogenases and instead catabolize atypical lipids called 4-hydroxy acids 🤯 doi.org/10.1038/s415...
ACAD10 and ACAD11 enable mammalian 4-hydroxy acid lipid catabolism - Nature Structural & Molecular Biology
Rashan, Bartlett and colleagues show that mammalian 4-hydroxy fatty acids are primarily catabolized by ACAD10 and ACAD11 (atypical mitochondrial and peroxisomal acyl-CoA dehydrogenases, respectively) ...
doi.org
June 22, 2025 at 11:30 PM
AND IT'S DONE! A 🧵 on our recent article now out in @natsmb.nature.com! Co-led with @abbybartlett.bsky.social, Pagliarini Lab, @judisimcox.bsky.social, we find ACAD10/11 are NOT like other acyl-CoA dehydrogenases and instead catabolize atypical lipids called 4-hydroxy acids 🤯 doi.org/10.1038/s415...
Reposted by Zak Baker
Very excited to share our latest work, “Mitochondrial presequences harbor variable strengths to maintain organellar function,” led by the fantastically talented @washubbsb.bsky.social graduate student Youmian Yan. A 🧵
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Mitochondrial presequences harbor variable strengths to maintain organellar function
Hundreds of mitochondrial-destined proteins rely on N-terminal presequences for organellar targeting and import. While generally described as positively charged amphipathic helices, presequences lack ...
www.biorxiv.org
May 29, 2025 at 9:18 PM
Very excited to share our latest work, “Mitochondrial presequences harbor variable strengths to maintain organellar function,” led by the fantastically talented @washubbsb.bsky.social graduate student Youmian Yan. A 🧵
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted by Zak Baker
Thrilled to have our story on the regulation of lysosomal lipids out in @cp-cellmetabolism.bsky.social! The work was led by two outstanding grad students Jess Davidson and Raghav Jain probing mechanistic control of energy expenditure @hhmi.org @uwbiochem.bsky.social
Online now: Hepatic lipid remodeling in cold exposure uncovers direct regulation of bis(monoacylglycero)phosphate lipids by phospholipase A2 group XV
Hepatic lipid remodeling in cold exposure uncovers direct regulation of bis(monoacylglycero)phosphate lipids by phospholipase A2 group XV
Davidson et al. demonstrated that liver lysosomes orchestrate the metabolic adaptation to cold by rewiring lipid processing. They show that phospholipase A2 group XV (PLA2G15) breaks down bis(monoacylglycerol)phosphate (BMP) lipids to shift lysosomal function and metabolism in response to direct transcription factor EB regulation.
dlvr.it
May 15, 2025 at 12:06 PM
Thrilled to have our story on the regulation of lysosomal lipids out in @cp-cellmetabolism.bsky.social! The work was led by two outstanding grad students Jess Davidson and Raghav Jain probing mechanistic control of energy expenditure @hhmi.org @uwbiochem.bsky.social
Reposted by Zak Baker
excited to share our latest work now online
@natmetabolism.nature.com, led by
@kyle-flickinger.bsky.social, where we unravel a mechanistic basis for the conditional essentiality of NADK, one of the many interesting hits from our previously reported CRISPR screening with HPLM rdcu.be/ekpu6
@natmetabolism.nature.com, led by
@kyle-flickinger.bsky.social, where we unravel a mechanistic basis for the conditional essentiality of NADK, one of the many interesting hits from our previously reported CRISPR screening with HPLM rdcu.be/ekpu6
May 2, 2025 at 3:32 PM
excited to share our latest work now online
@natmetabolism.nature.com, led by
@kyle-flickinger.bsky.social, where we unravel a mechanistic basis for the conditional essentiality of NADK, one of the many interesting hits from our previously reported CRISPR screening with HPLM rdcu.be/ekpu6
@natmetabolism.nature.com, led by
@kyle-flickinger.bsky.social, where we unravel a mechanistic basis for the conditional essentiality of NADK, one of the many interesting hits from our previously reported CRISPR screening with HPLM rdcu.be/ekpu6
Reposted by Zak Baker
Important to celebrate trainee victories: excited to have Holly Merta's @hollymerta.bsky.social paper out!
We use TurboID sub-organelle proteomics to map the ER network.
We find calmin/CLMN, an ER-to-actin tether regulating focal adhesions & cell motility.
www.sciencedirect.com/science/arti...
We use TurboID sub-organelle proteomics to map the ER network.
We find calmin/CLMN, an ER-to-actin tether regulating focal adhesions & cell motility.
www.sciencedirect.com/science/arti...
Spatial proteomics of ER tubules reveals CLMN, an ER-actin tether at focal adhesions that promotes cell migration
The endoplasmic reticulum (ER) is structurally and functionally diverse, yet how its functions are organized within morphological subdomains is incomp…
www.sciencedirect.com
April 7, 2025 at 1:07 PM
Important to celebrate trainee victories: excited to have Holly Merta's @hollymerta.bsky.social paper out!
We use TurboID sub-organelle proteomics to map the ER network.
We find calmin/CLMN, an ER-to-actin tether regulating focal adhesions & cell motility.
www.sciencedirect.com/science/arti...
We use TurboID sub-organelle proteomics to map the ER network.
We find calmin/CLMN, an ER-to-actin tether regulating focal adhesions & cell motility.
www.sciencedirect.com/science/arti...
Reposted by Zak Baker
🍰Interested in reading more about the study? Here's the Research Briefing:
👉🏼https://rdcu.be/eadgo
www.nature.com/articles/s41...
👉🏼https://rdcu.be/eadgo
www.nature.com/articles/s41...
Multiomics reveals that triacylglycerol mobilization helps drive recovery from mitochondrial stress - Nature Cell Biology
We devised a multiomics strategy to identify metabolic pathways used by yeast to surmount mitochondrial stress. Strains capable of recovery relied on triacylglycerol mobilization to provide acyl group...
www.nature.com
February 17, 2025 at 2:48 PM
🍰Interested in reading more about the study? Here's the Research Briefing:
👉🏼https://rdcu.be/eadgo
www.nature.com/articles/s41...
👉🏼https://rdcu.be/eadgo
www.nature.com/articles/s41...
Reposted by Zak Baker
💫NEW: @zakbaker.bsky.social, Pagliarini & co show that #mitochondria stress recovery requires mobilization of #LipidDroplet triacylglycerol stores to facilitate #cardiolipin biosynthesis and mitochondrial biogenesis.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Triacylglycerol mobilization underpins mitochondrial stress recovery - Nature Cell Biology
Baker et al. show that mitochondrial stress recovery requires mobilization of lipid droplet triacylglycerol stores to facilitate cardiolipin biosynthesis and mitochondrial biogenesis.
www.nature.com
February 17, 2025 at 2:48 PM
💫NEW: @zakbaker.bsky.social, Pagliarini & co show that #mitochondria stress recovery requires mobilization of #LipidDroplet triacylglycerol stores to facilitate #cardiolipin biosynthesis and mitochondrial biogenesis.
www.nature.com/articles/s41...
www.nature.com/articles/s41...