Finally, a special thank you to Emilia Bianchini (co-first author) and Gilad Evrony (PI/mentor) for all your help! I am thrilled to see this come out!

Here is an open-access link to bypass the paywall. :) authors.elsevier.com/c/1kg6b9UKeT...

We accomplished this in an average of ~15 min for the IDH1 R132H assay and ~17 min for the BRAF V600E assay. Further, we combined UR-ddPCR with UR-stimulated Raman histology to estimate the tumor cell density from the tumor core and boundary (4/5)

We then implemented UR-ddPCR in the operating room successfully genotyping and quantifying the tumor cell percentage of 78 tumor samples across 22 OR cases (9 added since our pre-print). (3/5)

We developed an ultra-rapid, ddPCR-compatible DNA extraction (~3 minutes) and an ultra-rapid thermal cycling method (~5 minutes) which allowed us to create UR-ddPCR assay for both the IDH1 R132H and BRAF V600E mutations that were highly concordant with standard ddPCR (R^2 = .995) (2/5)