@xipeng1.bsky.social
Triangle-Beam Interference SIM (3I-SIM)—a method replaces rotating stripes with hexagonal lattice. This innovation slashes redundancy and unlocks breathtaking speed. The system achieves imaging at up to 1,697 Hz, 13 hours, 100,000 snapshots in action: www.sciencenewstoday.org/the-secret-w...
The Secret World of Living Cells Comes Alive With a New Microscope
For as long as humans have peered through lenses, the microscope has been our gateway to hidden worlds. From Robert…
www.sciencenewstoday.org
October 13, 2025 at 2:18 AM
Triangle-Beam Interference SIM (3I-SIM)—a method replaces rotating stripes with hexagonal lattice. This innovation slashes redundancy and unlocks breathtaking speed. The system achieves imaging at up to 1,697 Hz, 13 hours, 100,000 snapshots in action: www.sciencenewstoday.org/the-secret-w...
Reposted
Absolutely beautiful paper with some awesome imaging of our AC-ER probe for monitoring ER-associated actin (PMID: 32778832) 🤯
August 19, 2025 at 3:20 PM
Absolutely beautiful paper with some awesome imaging of our AC-ER probe for monitoring ER-associated actin (PMID: 32778832) 🤯
Very proud to be the first PKU Talks interviewed professor!😀😀😀Come and take a look!
🔬Ever wondered what a microscopy pioneer would pack for a one-way Mars trip?
Dive into our debut episode of “Walk This Way: PKU Talks” with Prof. Xi Peng—strolling through his lab, sharing SIM breakthroughs, his favorite movie, and more...
📺: www.youtube.com/watch?v=tq-q...
@xipeng1.bsky.social
Dive into our debut episode of “Walk This Way: PKU Talks” with Prof. Xi Peng—strolling through his lab, sharing SIM breakthroughs, his favorite movie, and more...
📺: www.youtube.com/watch?v=tq-q...
@xipeng1.bsky.social
October 4, 2025 at 1:30 PM
Very proud to be the first PKU Talks interviewed professor!😀😀😀Come and take a look!
Nat. Photonics🔬 Meet 3I-SIM!
Instead of stripes, we use 3-beam to generate 2D hex pattern for simultaneous 2D SIM modulation, with >100,000 frames, 13 h neuronal cell movies, ER–lipid-droplet kiss-n-run and millisecond tango. All hard&software open.
doi.org/10.1038/s415... @pku1898.bsky.social
Instead of stripes, we use 3-beam to generate 2D hex pattern for simultaneous 2D SIM modulation, with >100,000 frames, 13 h neuronal cell movies, ER–lipid-droplet kiss-n-run and millisecond tango. All hard&software open.
doi.org/10.1038/s415... @pku1898.bsky.social
Triangle-beam interference structured illumination microscopy - Nature Photonics
Triangle-beam interference structured illumination microscopy leverages radially polarized beams to generate two-dimensional lattice illumination patterns. The technique enables a temporal resolution ...
doi.org
August 15, 2025 at 11:24 AM
Nat. Photonics🔬 Meet 3I-SIM!
Instead of stripes, we use 3-beam to generate 2D hex pattern for simultaneous 2D SIM modulation, with >100,000 frames, 13 h neuronal cell movies, ER–lipid-droplet kiss-n-run and millisecond tango. All hard&software open.
doi.org/10.1038/s415... @pku1898.bsky.social
Instead of stripes, we use 3-beam to generate 2D hex pattern for simultaneous 2D SIM modulation, with >100,000 frames, 13 h neuronal cell movies, ER–lipid-droplet kiss-n-run and millisecond tango. All hard&software open.
doi.org/10.1038/s415... @pku1898.bsky.social
C²SD-ISM combines physical defocus rejection (spinning disk) with adaptive algorithm (DPA-PR), breaking depth limits in tissue super-resolution (↑180 μm), enhances fidelity (92% linear correlation), and supports high-throughput imaging. doi.org/10.1038/s413... Github: github.com/Chauncey-Leu....
High-fidelity tissue super-resolution imaging achieved with confocal2 spinning-disk image scanning microscopy - Light: Science & Applications
C²SD-ISM integrates an SD and a DMD to form a dual confocal configuration, enabling deep-tissue super-resolution imaging with DPA-PR reconstruction.
doi.org
August 4, 2025 at 2:01 PM
C²SD-ISM combines physical defocus rejection (spinning disk) with adaptive algorithm (DPA-PR), breaking depth limits in tissue super-resolution (↑180 μm), enhances fidelity (92% linear correlation), and supports high-throughput imaging. doi.org/10.1038/s413... Github: github.com/Chauncey-Leu....
Reposted
mScarlet3-H (aka mYongHong) is on the cover of Nature Methods! All plasmids are available for free from WeKwikGene wekwikgene.wllsb.edu.cn/publications...
June 18, 2025 at 12:16 AM
mScarlet3-H (aka mYongHong) is on the cover of Nature Methods! All plasmids are available for free from WeKwikGene wekwikgene.wllsb.edu.cn/publications...
Congratulations to Suyi Zhong, Wei Ren, Ziyi Yang, and Hongyu Wang!
June 19, 2025 at 5:25 AM
Congratulations to Suyi Zhong, Wei Ren, Ziyi Yang, and Hongyu Wang!
I am very glad to see that our PolarSIM helps the leading scientists to observe richer dynamics and get more discovery! Congratulations to Zhixing team for this brand new palette of fluorescent dyes named after BeiDa! www.nature.com/articles/s41... @pku1898.bsky.social @zhixingchen2.bsky.social
May 30, 2025 at 8:25 AM
I am very glad to see that our PolarSIM helps the leading scientists to observe richer dynamics and get more discovery! Congratulations to Zhixing team for this brand new palette of fluorescent dyes named after BeiDa! www.nature.com/articles/s41... @pku1898.bsky.social @zhixingchen2.bsky.social
Reposted
Here come our new palette of fluorescent dyes. The BD dyes endeaves to balance glowy brightness, robust photostability, and biocompatibility. Let's boost 4D dynamic super-resolution imaging!!!
www.nature.com/articles/s41...
@spirochrome.com
www.nature.com/articles/s41...
@spirochrome.com
A palette of bridged bicycle-strengthened fluorophores - Nature Methods
A suite of bridged rhodamine dyes (BriDyes) offers excellent brightness, solubility, photostability, and tunable cell permeability along with resistance to photoblueing, making them exceptional all-pu...
www.nature.com
May 20, 2025 at 2:11 PM
Here come our new palette of fluorescent dyes. The BD dyes endeaves to balance glowy brightness, robust photostability, and biocompatibility. Let's boost 4D dynamic super-resolution imaging!!!
www.nature.com/articles/s41...
@spirochrome.com
www.nature.com/articles/s41...
@spirochrome.com
I am very glad to see that our PolarSIM helps the leading scientists to observe richer dynamics and get more discovery! Congratulations to Zhixing team for this brand new palette of fluorescent dyes named after BeiDa! www.nature.com/articles/s41... @pku1898.bsky.social @zhixingchen2.bsky.social
May 30, 2025 at 8:25 AM
I am very glad to see that our PolarSIM helps the leading scientists to observe richer dynamics and get more discovery! Congratulations to Zhixing team for this brand new palette of fluorescent dyes named after BeiDa! www.nature.com/articles/s41... @pku1898.bsky.social @zhixingchen2.bsky.social
I am very glad to see that our PolarSIM helps the leading scientists to observe richer dynamics and get more discovery! Congratulations to Zhixing team for this brand new palette of fluorescent dyes named after BeiDa! @pku1898.bsky.social @zhixingchen2.bsky.social
May 30, 2025 at 8:22 AM
I am very glad to see that our PolarSIM helps the leading scientists to observe richer dynamics and get more discovery! Congratulations to Zhixing team for this brand new palette of fluorescent dyes named after BeiDa! @pku1898.bsky.social @zhixingchen2.bsky.social
I am very glad to see that our PolarSIM helps the leading scientists to observe richer dynamics and get more discovery! Congratulations to Zhixing team for this brand new palette of fluorescent dyes named after BeiDa! @pku1898.bsky.social @zhixingchen2.bsky.social
Here come our new palette of fluorescent dyes. The BD dyes endeaves to balance glowy brightness, robust photostability, and biocompatibility. Let's boost 4D dynamic super-resolution imaging!!!
www.nature.com/articles/s41...
@spirochrome.com
www.nature.com/articles/s41...
@spirochrome.com
A palette of bridged bicycle-strengthened fluorophores - Nature Methods
A suite of bridged rhodamine dyes (BriDyes) offers excellent brightness, solubility, photostability, and tunable cell permeability along with resistance to photoblueing, making them exceptional all-pu...
www.nature.com
May 30, 2025 at 8:20 AM
I am very glad to see that our PolarSIM helps the leading scientists to observe richer dynamics and get more discovery! Congratulations to Zhixing team for this brand new palette of fluorescent dyes named after BeiDa! @pku1898.bsky.social @zhixingchen2.bsky.social
Thrilled to introduce our Cover review: High-spatiotemporal-resolution structured illumination microscopy: principles, instrumentation, and applications dx.doi.org/10.3788/PI.2...
May 30, 2025 at 6:57 AM
Thrilled to introduce our Cover review: High-spatiotemporal-resolution structured illumination microscopy: principles, instrumentation, and applications dx.doi.org/10.3788/PI.2...
Unlock the secrets of cellular dynamics with high-spatiotemporal-resolution structured illumination microscopy! Our latest review explores the principles, instrumentation, and applications of SIM&ISM in live-cell imaging. #Microscopy #SuperResolution #CellBiology dx.doi.org/10.3788/PI.2...
May 29, 2025 at 1:38 AM
Unlock the secrets of cellular dynamics with high-spatiotemporal-resolution structured illumination microscopy! Our latest review explores the principles, instrumentation, and applications of SIM&ISM in live-cell imaging. #Microscopy #SuperResolution #CellBiology dx.doi.org/10.3788/PI.2...
Reposted
Seeing Deeper, Clearer than Ever!🔬Prof. Xi Peng’s team from #PKU College of Future Technology introduces Dark Sectioning in Nature Methods—a breakthrough for fluorescence microscopy!
A peek into the future of imaging:
www.nature.com/articles/s41...
#PKUResearch #AcademicSky @natmethods.nature.com
A peek into the future of imaging:
www.nature.com/articles/s41...
#PKUResearch #AcademicSky @natmethods.nature.com
May 27, 2025 at 3:01 AM
Seeing Deeper, Clearer than Ever!🔬Prof. Xi Peng’s team from #PKU College of Future Technology introduces Dark Sectioning in Nature Methods—a breakthrough for fluorescence microscopy!
A peek into the future of imaging:
www.nature.com/articles/s41...
#PKUResearch #AcademicSky @natmethods.nature.com
A peek into the future of imaging:
www.nature.com/articles/s41...
#PKUResearch #AcademicSky @natmethods.nature.com
Reposted
For a quick summary of the paper, read the Research Briefing here:
www.nature.com/articles/s41...
www.nature.com/articles/s41...
May 13, 2025 at 1:59 PM
For a quick summary of the paper, read the Research Briefing here:
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted
Nice work! We integrated this dark-sectioning method into our FlexSIM pipeline (github.com/esoubies/Fle...) as an additional option for handling out-of-focus signal. It works remarkably well!
A Dark sectioning algorithm removes background and provides single-frame optical sectioning in fluorescence microscopy, facilitating improved deep-tissue segmentation accuracy.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Dark-based optical sectioning assists background removal in fluorescence microscopy - Nature Methods
The Dark sectioning algorithm removes the background and provides single-frame optical sectioning in fluorescence microscopy. It offers improved quantitative analysis and deep-tissue segmentation accu...
www.nature.com
May 15, 2025 at 12:30 PM
Nice work! We integrated this dark-sectioning method into our FlexSIM pipeline (github.com/esoubies/Fle...) as an additional option for handling out-of-focus signal. It works remarkably well!
Reposted
In particular, it handles out-of-focus regions more effectively than traditional OTF attenuation.
May 15, 2025 at 12:31 PM
In particular, it handles out-of-focus regions more effectively than traditional OTF attenuation.
Our groundbreaking Dark Sectioning technique is now published in Nature Methods! 🎉 It supercharges existing imaging systems:
🔬 Widefield → Confocal
🔬 Single-photon → Multiphoton
🔬 2D-SIM → 3D-SIM
Now integrated in Airy PolarSIM and #FlexSIM!
doi.org/10.1038/s415...
#Microscopy #Fluorescence
🔬 Widefield → Confocal
🔬 Single-photon → Multiphoton
🔬 2D-SIM → 3D-SIM
Now integrated in Airy PolarSIM and #FlexSIM!
doi.org/10.1038/s415...
#Microscopy #Fluorescence
May 16, 2025 at 3:03 AM
Our groundbreaking Dark Sectioning technique is now published in Nature Methods! 🎉 It supercharges existing imaging systems:
🔬 Widefield → Confocal
🔬 Single-photon → Multiphoton
🔬 2D-SIM → 3D-SIM
Now integrated in Airy PolarSIM and #FlexSIM!
doi.org/10.1038/s415...
#Microscopy #Fluorescence
🔬 Widefield → Confocal
🔬 Single-photon → Multiphoton
🔬 2D-SIM → 3D-SIM
Now integrated in Airy PolarSIM and #FlexSIM!
doi.org/10.1038/s415...
#Microscopy #Fluorescence