Wythe Lab at UVA School of Medicine
@wythelab.bsky.social
Vascular biologist. Brain AVMs and Kras, glioblastoma and angiogenesis, lymphatics, and developmental biology.
https://www.wythelab.com
https://www.wythelab.com
Just let us know what you need!
November 15, 2025 at 1:08 AM
Just let us know what you need!
Reposted by Wythe Lab at UVA School of Medicine
"The Cavalier Daily corresponded with former Rector Robert Hardie, whose term ended June 30, regarding the two accounts. He confirmed that he agreed with Ryan’s account.
'I concur fully with President Ryan’s recollection of events,' Hardie said."
Welp
www.cavalierdaily.com/article/2025...
'I concur fully with President Ryan’s recollection of events,' Hardie said."
Welp
www.cavalierdaily.com/article/2025...
Ryan and Sheridan provide full accounts of Ryan’s resignation. They differ significantly
“I concur fully with President Ryan’s recollection of events,” former Board Rector Robert Hardie said.
www.cavalierdaily.com
November 14, 2025 at 7:46 PM
"The Cavalier Daily corresponded with former Rector Robert Hardie, whose term ended June 30, regarding the two accounts. He confirmed that he agreed with Ryan’s account.
'I concur fully with President Ryan’s recollection of events,' Hardie said."
Welp
www.cavalierdaily.com/article/2025...
'I concur fully with President Ryan’s recollection of events,' Hardie said."
Welp
www.cavalierdaily.com/article/2025...
We love us some @addgene.bsky.social !!
November 14, 2025 at 8:07 PM
We love us some @addgene.bsky.social !!
While not a lot of the tools are new (and shoutout to the folks who made the original templates we cloned from), we hope shuttling them all into a common Gateway system that uses the same logic and the zebrafish Tol2, Tol1, and Phi31 systems is of use for other labs. So....
a man with a green hat says come and get it
Alt: FAK from The Bear says "come and get it!".
media.tenor.com
November 14, 2025 at 5:33 PM
While not a lot of the tools are new (and shoutout to the folks who made the original templates we cloned from), we hope shuttling them all into a common Gateway system that uses the same logic and the zebrafish Tol2, Tol1, and Phi31 systems is of use for other labs. So....
Or need an all in one / single vector Hot-Cre system for experiments in zebrafish?
Got that too!
Got that too!
November 14, 2025 at 5:33 PM
Or need an all in one / single vector Hot-Cre system for experiments in zebrafish?
Got that too!
Got that too!
Like piggybac transposons for in utero electroporation to generate brain tumors, or express other constructs?
We got you!
We got you!
November 14, 2025 at 5:33 PM
Like piggybac transposons for in utero electroporation to generate brain tumors, or express other constructs?
We got you!
We got you!
Interested in Cre or Dre recombinases? need FP-Cre or FP-2A-Cre fusions?
Need Dre FP switch reporter vectors or rox flanked stop cassettes?
Need Dre FP switch reporter vectors or rox flanked stop cassettes?
a man in a suit says come this way in black letters
Alt: a man in a suit says come this way in black letters
media.tenor.com
November 14, 2025 at 5:33 PM
Interested in Cre or Dre recombinases? need FP-Cre or FP-2A-Cre fusions?
Need Dre FP switch reporter vectors or rox flanked stop cassettes?
Need Dre FP switch reporter vectors or rox flanked stop cassettes?
We also optimized and compared several piggybac Tet-On vectors for in vitro use, as well as tet-on/off, and FLEX/DIO vectors for AAV and lenti use
November 14, 2025 at 5:33 PM
We also optimized and compared several piggybac Tet-On vectors for in vitro use, as well as tet-on/off, and FLEX/DIO vectors for AAV and lenti use
Interested in Tet-inducible systems?
We include middle entry clones containing rtTA, as well as 3' rtTA clones.
We include middle entry clones containing rtTA, as well as 3' rtTA clones.
November 14, 2025 at 5:33 PM
Interested in Tet-inducible systems?
We include middle entry clones containing rtTA, as well as 3' rtTA clones.
We include middle entry clones containing rtTA, as well as 3' rtTA clones.
We include new variants with IRES FP reporters as well!
November 14, 2025 at 5:33 PM
We include new variants with IRES FP reporters as well!
Need some strong single site vectors for expression in mammalian cells?
Like pCAGGs or pCAGEN?
We have several DEST vectors you can use that outperform pCS2!
Like pCAGGs or pCAGEN?
We have several DEST vectors you can use that outperform pCS2!
November 14, 2025 at 5:33 PM
Need some strong single site vectors for expression in mammalian cells?
Like pCAGGs or pCAGEN?
We have several DEST vectors you can use that outperform pCS2!
Like pCAGGs or pCAGEN?
We have several DEST vectors you can use that outperform pCS2!
We optimize the lexA/lexO system, and make it all compatible in a single vector system, with faster off kinetics via destablization domains and more changes.
November 14, 2025 at 5:33 PM
We optimize the lexA/lexO system, and make it all compatible in a single vector system, with faster off kinetics via destablization domains and more changes.
You looking for chemically inducible expression in zebrafish?
a man stands on a football field holding a microphone with the words right here above him
Alt: the rock, doing rock-ish things, saying "right here"
media.tenor.com
November 14, 2025 at 5:33 PM
You looking for chemically inducible expression in zebrafish?
In vivo LiCre middle entry clones and validation in mice, and Gateway compatible nTAEL2.0 (developed by the Woo lab), and more.
November 14, 2025 at 5:33 PM
In vivo LiCre middle entry clones and validation in mice, and Gateway compatible nTAEL2.0 (developed by the Woo lab), and more.
Want optical control of Cre activity, or of gene expression?
November 14, 2025 at 5:33 PM
Want optical control of Cre activity, or of gene expression?
Need cell cycle indicators for use in fish or in mammals?
November 14, 2025 at 5:33 PM
Need cell cycle indicators for use in fish or in mammals?
Need subcellular reporters like lifeact, actin-nanobodies, laminin nanobodies, Histone fusions, Sun1, and more?
Want Halo tags or other FPs? mScarlet-I? mScarlet3-H? mBaojin? StayGold? mCerulean2? NeonGreen?
Say no more!
Want Halo tags or other FPs? mScarlet-I? mScarlet3-H? mBaojin? StayGold? mCerulean2? NeonGreen?
Say no more!
November 14, 2025 at 5:33 PM
Need subcellular reporters like lifeact, actin-nanobodies, laminin nanobodies, Histone fusions, Sun1, and more?
Want Halo tags or other FPs? mScarlet-I? mScarlet3-H? mBaojin? StayGold? mCerulean2? NeonGreen?
Say no more!
Want Halo tags or other FPs? mScarlet-I? mScarlet3-H? mBaojin? StayGold? mCerulean2? NeonGreen?
Say no more!
We also include a FileMaker relational database for plasmid entry and record keeping!
You can find the database at Development in the supplementary materials, or at our website:
www.wythelab.com/wythe-lab-da...
In the supp materials we include videos that show how to use the database as well.
You can find the database at Development in the supplementary materials, or at our website:
www.wythelab.com/wythe-lab-da...
In the supp materials we include videos that show how to use the database as well.
November 14, 2025 at 5:33 PM
We also include a FileMaker relational database for plasmid entry and record keeping!
You can find the database at Development in the supplementary materials, or at our website:
www.wythelab.com/wythe-lab-da...
In the supp materials we include videos that show how to use the database as well.
You can find the database at Development in the supplementary materials, or at our website:
www.wythelab.com/wythe-lab-da...
In the supp materials we include videos that show how to use the database as well.
Then you're in for a treat!
>140 plasmids (some still undergoing QC), all freely available at Addgene:
www.addgene.org/browse/artic...
>140 plasmids (some still undergoing QC), all freely available at Addgene:
www.addgene.org/browse/artic...
Addgene: Multisite Assembly of Gateway Induced Clones (MAGIC): a flexible cloning toolbox with diverse applications in vertebrate model systems.
www.addgene.org
November 14, 2025 at 5:33 PM
Then you're in for a treat!
>140 plasmids (some still undergoing QC), all freely available at Addgene:
www.addgene.org/browse/artic...
>140 plasmids (some still undergoing QC), all freely available at Addgene:
www.addgene.org/browse/artic...
Do you do transgenesis in zebrafish? in mice? in chicken? cell culture models? AAV? Piggybac? Lentivirus? Tol2? I-Sce?
Do you use multisite or single site Gateway cloning?
Then....
Do you use multisite or single site Gateway cloning?
Then....
a man in a plaid shirt and tie is standing next to a boy in a green shirt .
Alt: The legend, Chris Farley, getting excited.
media.tenor.com
November 14, 2025 at 5:33 PM
Do you do transgenesis in zebrafish? in mice? in chicken? cell culture models? AAV? Piggybac? Lentivirus? Tol2? I-Sce?
Do you use multisite or single site Gateway cloning?
Then....
Do you use multisite or single site Gateway cloning?
Then....