Wythe Lab at UVA School of Medicine
@wythelab.bsky.social
Vascular biologist. Brain AVMs and Kras, glioblastoma and angiogenesis, lymphatics, and developmental biology.
https://www.wythelab.com
https://www.wythelab.com
Or need an all in one / single vector Hot-Cre system for experiments in zebrafish?
Got that too!
Got that too!
November 14, 2025 at 5:33 PM
Or need an all in one / single vector Hot-Cre system for experiments in zebrafish?
Got that too!
Got that too!
Like piggybac transposons for in utero electroporation to generate brain tumors, or express other constructs?
We got you!
We got you!
November 14, 2025 at 5:33 PM
Like piggybac transposons for in utero electroporation to generate brain tumors, or express other constructs?
We got you!
We got you!
We also optimized and compared several piggybac Tet-On vectors for in vitro use, as well as tet-on/off, and FLEX/DIO vectors for AAV and lenti use
November 14, 2025 at 5:33 PM
We also optimized and compared several piggybac Tet-On vectors for in vitro use, as well as tet-on/off, and FLEX/DIO vectors for AAV and lenti use
Interested in Tet-inducible systems?
We include middle entry clones containing rtTA, as well as 3' rtTA clones.
We include middle entry clones containing rtTA, as well as 3' rtTA clones.
November 14, 2025 at 5:33 PM
Interested in Tet-inducible systems?
We include middle entry clones containing rtTA, as well as 3' rtTA clones.
We include middle entry clones containing rtTA, as well as 3' rtTA clones.
We include new variants with IRES FP reporters as well!
November 14, 2025 at 5:33 PM
We include new variants with IRES FP reporters as well!
Need some strong single site vectors for expression in mammalian cells?
Like pCAGGs or pCAGEN?
We have several DEST vectors you can use that outperform pCS2!
Like pCAGGs or pCAGEN?
We have several DEST vectors you can use that outperform pCS2!
November 14, 2025 at 5:33 PM
Need some strong single site vectors for expression in mammalian cells?
Like pCAGGs or pCAGEN?
We have several DEST vectors you can use that outperform pCS2!
Like pCAGGs or pCAGEN?
We have several DEST vectors you can use that outperform pCS2!
We optimize the lexA/lexO system, and make it all compatible in a single vector system, with faster off kinetics via destablization domains and more changes.
November 14, 2025 at 5:33 PM
We optimize the lexA/lexO system, and make it all compatible in a single vector system, with faster off kinetics via destablization domains and more changes.
In vivo LiCre middle entry clones and validation in mice, and Gateway compatible nTAEL2.0 (developed by the Woo lab), and more.
November 14, 2025 at 5:33 PM
In vivo LiCre middle entry clones and validation in mice, and Gateway compatible nTAEL2.0 (developed by the Woo lab), and more.
Want optical control of Cre activity, or of gene expression?
November 14, 2025 at 5:33 PM
Want optical control of Cre activity, or of gene expression?
Need cell cycle indicators for use in fish or in mammals?
November 14, 2025 at 5:33 PM
Need cell cycle indicators for use in fish or in mammals?
Need subcellular reporters like lifeact, actin-nanobodies, laminin nanobodies, Histone fusions, Sun1, and more?
Want Halo tags or other FPs? mScarlet-I? mScarlet3-H? mBaojin? StayGold? mCerulean2? NeonGreen?
Say no more!
Want Halo tags or other FPs? mScarlet-I? mScarlet3-H? mBaojin? StayGold? mCerulean2? NeonGreen?
Say no more!
November 14, 2025 at 5:33 PM
Need subcellular reporters like lifeact, actin-nanobodies, laminin nanobodies, Histone fusions, Sun1, and more?
Want Halo tags or other FPs? mScarlet-I? mScarlet3-H? mBaojin? StayGold? mCerulean2? NeonGreen?
Say no more!
Want Halo tags or other FPs? mScarlet-I? mScarlet3-H? mBaojin? StayGold? mCerulean2? NeonGreen?
Say no more!
We also include a FileMaker relational database for plasmid entry and record keeping!
You can find the database at Development in the supplementary materials, or at our website:
www.wythelab.com/wythe-lab-da...
In the supp materials we include videos that show how to use the database as well.
You can find the database at Development in the supplementary materials, or at our website:
www.wythelab.com/wythe-lab-da...
In the supp materials we include videos that show how to use the database as well.
November 14, 2025 at 5:33 PM
We also include a FileMaker relational database for plasmid entry and record keeping!
You can find the database at Development in the supplementary materials, or at our website:
www.wythelab.com/wythe-lab-da...
In the supp materials we include videos that show how to use the database as well.
You can find the database at Development in the supplementary materials, or at our website:
www.wythelab.com/wythe-lab-da...
In the supp materials we include videos that show how to use the database as well.
Laser tag fort coming together nicely.
November 2, 2025 at 7:12 PM
Laser tag fort coming together nicely.
Beautiful bike ride up the blue ridge parkway this am, but steep! The. A fun hike and building forts for laser tag battles with the kid and his buddy
November 2, 2025 at 7:12 PM
Beautiful bike ride up the blue ridge parkway this am, but steep! The. A fun hike and building forts for laser tag battles with the kid and his buddy
Wouldn’t call 1989 “a perfect music year” by any stretch, but some bangers for sure. Honorable mention to de la soul, NWA, Beattie boys for Paul’s boutique, and fugazi.
October 30, 2025 at 12:45 AM
Wouldn’t call 1989 “a perfect music year” by any stretch, but some bangers for sure. Honorable mention to de la soul, NWA, Beattie boys for Paul’s boutique, and fugazi.
Beautiful day for a hike in the Blue Ridge (humpback rock)
October 26, 2025 at 10:04 PM
Beautiful day for a hike in the Blue Ridge (humpback rock)
No, the kid isn’t excited for Halloween at all. Why do you ask?
October 26, 2025 at 1:52 AM
No, the kid isn’t excited for Halloween at all. Why do you ask?
Headed home after a very stimulating @navbo.bsky.social meeting. Great to see friends and meet new folks in the vascular biology field. And proud of Gabrielle for giving a great talk on her work on Kras (according to my spies in the audience, I had to leave before the last session).
October 23, 2025 at 10:38 PM
Headed home after a very stimulating @navbo.bsky.social meeting. Great to see friends and meet new folks in the vascular biology field. And proud of Gabrielle for giving a great talk on her work on Kras (according to my spies in the audience, I had to leave before the last session).
Good day for a protest against the “compact”
October 17, 2025 at 9:30 PM
Good day for a protest against the “compact”
Great day at the UVA neuroscience graduate program (NGP) retreat. Chalk talks from second years, new faculty talks, shark tank, and poster session, and a great venue (Maren Estate). All organized by students!
October 15, 2025 at 11:07 PM
Great day at the UVA neuroscience graduate program (NGP) retreat. Chalk talks from second years, new faculty talks, shark tank, and poster session, and a great venue (Maren Estate). All organized by students!
Great talk by Azeez in the lab at the annual NIH REJOIN meeting on joint nerve and vessel innervation in osteoarthritis in Houston this afternoon.
October 2, 2025 at 7:40 PM
Great talk by Azeez in the lab at the annual NIH REJOIN meeting on joint nerve and vessel innervation in osteoarthritis in Houston this afternoon.
Excited for the annual Anderson lecture from Huda Zoghbi at Baylor college of medicine today at UVA
September 22, 2025 at 8:09 PM
Excited for the annual Anderson lecture from Huda Zoghbi at Baylor college of medicine today at UVA