Witold Szymański
@witek-teammassspec.bsky.social
mass spec | proteomics | EVs | cycling | climbing | running
Opinions here are my private!
I am a staff scientist at the Core Facility for Proteomics at the Phillips University of Marburg, Germany.
Website: graumannlab.science
Opinions here are my private!
I am a staff scientist at the Core Facility for Proteomics at the Phillips University of Marburg, Germany.
Website: graumannlab.science
SLS, SDC? You can precipitate them out in low pH.
April 14, 2025 at 5:00 PM
SLS, SDC? You can precipitate them out in low pH.
Yes, exactly.
April 7, 2025 at 5:42 PM
Yes, exactly.
Wait... What? I think we talk about different SP3 :) why expensive? Are you thinking about this? pubmed.ncbi.nlm.nih.gov/30464214/
Single-pot, solid-phase-enhanced sample preparation for proteomics experiments - PubMed
A critical step in proteomics analysis is the optimal extraction and processing of protein material to ensure the highest sensitivity in downstream detection. Achieving this requires a sample-handling technology that exhibits unbiased protein manipulation, flexibility in reagent use, and virtually l …
pubmed.ncbi.nlm.nih.gov
April 7, 2025 at 5:41 PM
Wait... What? I think we talk about different SP3 :) why expensive? Are you thinking about this? pubmed.ncbi.nlm.nih.gov/30464214/
Yes, that what I meant, with the "dirty" and "clean" samples. But the method of EV prep also affects the content in the sample, of the EV proteins, apart of the background.
April 7, 2025 at 1:51 PM
Yes, that what I meant, with the "dirty" and "clean" samples. But the method of EV prep also affects the content in the sample, of the EV proteins, apart of the background.
We use SP3 for the sample prep of EVs. We tested it against C18 many times and it was way better. We can wash away any detergent or salt and there is no carryover or effect on chromatography.
April 7, 2025 at 1:48 PM
We use SP3 for the sample prep of EVs. We tested it against C18 many times and it was way better. We can wash away any detergent or salt and there is no carryover or effect on chromatography.
But it's also a question to EV biologist, if they really expect to have >2000 proteins in their samples.
We commonly use higher % of SDS for the EV lysis, bit now I'd like to test the SDC :)
So many variables at play.
We commonly use higher % of SDS for the EV lysis, bit now I'd like to test the SDC :)
So many variables at play.
April 7, 2025 at 7:13 AM
But it's also a question to EV biologist, if they really expect to have >2000 proteins in their samples.
We commonly use higher % of SDS for the EV lysis, bit now I'd like to test the SDC :)
So many variables at play.
We commonly use higher % of SDS for the EV lysis, bit now I'd like to test the SDC :)
So many variables at play.
Nice and easy paper. However, the EV protein content is massively dependent on the isolation method. If I understood correctly, they "just" used UC, which is the "dirtiest" method. I wander how this whole comparison would look like with different EV preps: TFF, SEC, UF, combination of those?
April 7, 2025 at 7:10 AM
Nice and easy paper. However, the EV protein content is massively dependent on the isolation method. If I understood correctly, they "just" used UC, which is the "dirtiest" method. I wander how this whole comparison would look like with different EV preps: TFF, SEC, UF, combination of those?
Why not? They didn't want to come?
April 4, 2025 at 7:47 PM
Why not? They didn't want to come?
UK? 😏 Or does it still count as Europe?
April 3, 2025 at 8:21 AM
UK? 😏 Or does it still count as Europe?
How does it compare to Quarto? Worth a switch?
April 2, 2025 at 5:11 AM
How does it compare to Quarto? Worth a switch?
@kerrynugent51.bsky.social 's magic column continues to live. These Hela and BSA-QC runs are 1100 injections apart, running mainly with one-column methods on nanoElute2 from #bruker 🥳
April 1, 2025 at 11:21 AM
@kerrynugent51.bsky.social 's magic column continues to live. These Hela and BSA-QC runs are 1100 injections apart, running mainly with one-column methods on nanoElute2 from #bruker 🥳
What about that 24+k emails?! 🫠🤣
March 28, 2025 at 11:59 AM
What about that 24+k emails?! 🫠🤣
I already ordered all the missing chemicals 🫠
March 27, 2025 at 5:45 PM
I already ordered all the missing chemicals 🫠
Do you actually need to read and understand all this stuff? 😱😵💫🫠
March 26, 2025 at 9:47 PM
Do you actually need to read and understand all this stuff? 😱😵💫🫠
Merica!
https://media2.giphy.com/media/hXJ1MWMzY7Af32UIUD/200.gif
media2.giphy.com
March 26, 2025 at 10:57 AM
Merica!