Congratulations to 1st author ‪@ninasusannschmidt.bsky.social‬ and other group members + thanks to collaborators Profs. Herbert Schmidt @unihohenheim.bsky.social‬ and Christian Sina ‪@uniluebeck.bsky.social‬ and the entire INDICATE team (6/x)

Our findings suggest new opportunities for personalized, non-invasive risk assessments, e.g. by screening patients with predisposing cardiac conditions + increased infective endocarditis risks for prophylactic antibiotic treatment before they undergo invasive dental procedures (5/x)

Humans from two independent cohorts could be reproducibly stratified based on dominant uGI bacterial clusters, with a Prevotella7-dominated ‘salivatype’ being maintained in the duodenum and associated with reduced burdens of GI (Fusobacterium) and non-GI (HACEK) opportunistic pathogens and TNF (4/x)

The murine uGI microbiota contained more typical intestinal bacteria, which were transcriptionally inactive in the esophagus and active in the duodenum, possibly due to coprophagy and horizontal microbiome exchange between mice (3/x)

We used a DNA+RNA-based methodology to study the upper gastrointestinal of mice and humans, while carefully assessing contamination, providing a blueprint for other, low-microbiome biomass microbiome studies (2/x)

Maternal strain transfer to C-section infants appears to be delayed but not eliminated, as infants at age 2-10y share similar strain numbers with their mothers and fathers as vaginally delivered infants. (4/4)

Maternal strain transfer is reduced if mothers had antibiotics and/or C-section. C-section infants follow altered trajectory of microbiota maturation and exhibit delayed a transition from an oxygen-tolerant to intolerant species-dominated microbiota. (3/4)

Mostly intestinal maternal strains from Bacteroides spp. and Bifidobacterium spp. transfer to neonates during vaginal birth and persist into infancy; strains from Bacteroides spp. are even shared between parents and their grandparents. (2/4)

Metaanalysis by @podlesny.bsky.social of the neonatal #microbiome, maternal strain inheritance, and persistence into adolescence is out: doi.org/10.1016/j.ij.... We used our SameStr tool to identify and visualize shared strain networks. (1/4)

Hi Jan, could you please add me too. Thanks!

Our findings indicate microbiome-based diagnostic and TRF-based therapeutic applications that should be tested in IBD. - Special thanks to our collaborator Axel Lorentz @unihohenheim.bsky.social and the Baden-Wuerttemberg Foundation for funding. (7/x)

Interestingly, reduced gut microbiota rhythmicity and compositional changes preceded colitis symptoms in IL-10-/- mice, allowing us to train a PLS-DA model to predict colitis onset for individual mice from one experiment, which performed well on mice from a different experiment. (6/x)