Humans from two independent cohorts could be reproducibly stratified based on dominant uGI bacterial clusters, with a Prevotella7-dominated ‘salivatype’ being maintained in the duodenum and associated with reduced burdens of GI (Fusobacterium) and non-GI (HACEK) opportunistic pathogens and TNF (4/x)

The murine uGI microbiota contained more typical intestinal bacteria, which were transcriptionally inactive in the esophagus and active in the duodenum, possibly due to coprophagy and horizontal microbiome exchange between mice (3/x)

We used a DNA+RNA-based methodology to study the upper gastrointestinal of mice and humans, while carefully assessing contamination, providing a blueprint for other, low-microbiome biomass microbiome studies (2/x)

Interestingly, reduced gut microbiota rhythmicity and compositional changes preceded colitis symptoms in IL-10-/- mice, allowing us to train a PLS-DA model to predict colitis onset for individual mice from one experiment, which performed well on mice from a different experiment. (6/x)

Yet time-restricted feeding (TRF) had positive effects on IL-10-/- mice: It delayed colitis onset, reduced intestinal inflammation, improved the intestinal clock and increased gut microbiota rhythmicity - of the same taxa and to the same level as in wt mice. (5/x)

We found that while altered light/dark conditions disrupted the intestinal clock of wt mice, the intestinal clock and gut microbiota rhythmicity of IL-10-/- mice were impaired irrespective of external clock disruption, possibly due to dysbiosis even before the onset of colitis. (4/x)