Wee Boon Tan
@weeboont.bsky.social
Research Fellow studying phospholipid trafficking in diderm bacteria at National University of Singapore
Reposted by Wee Boon Tan
Bacterial cell widening alters periplasmic size and activates envelope stress responses
Kerwyn Huang, @typaslab.bsky.social et al find that E. coli outer-membrane protein RcsF senses reduced periplasm thickness due to increased cell width, & activates Rcs signaling
www.embopress.org/doi/full/10....
Kerwyn Huang, @typaslab.bsky.social et al find that E. coli outer-membrane protein RcsF senses reduced periplasm thickness due to increased cell width, & activates Rcs signaling
www.embopress.org/doi/full/10....
September 4, 2025 at 9:39 AM
Bacterial cell widening alters periplasmic size and activates envelope stress responses
Kerwyn Huang, @typaslab.bsky.social et al find that E. coli outer-membrane protein RcsF senses reduced periplasm thickness due to increased cell width, & activates Rcs signaling
www.embopress.org/doi/full/10....
Kerwyn Huang, @typaslab.bsky.social et al find that E. coli outer-membrane protein RcsF senses reduced periplasm thickness due to increased cell width, & activates Rcs signaling
www.embopress.org/doi/full/10....
Reposted by Wee Boon Tan
Thrilled to see my first publication on food chain biofilms out! Check out the super cool biofilms Pseudomonas makes on food surfaces and the contribution of these to food spoilage. We're continuing to work in this space to find novel ways to extend food shelf life and reduce food waste! 🧫🧪
Tissue destruction during food spoilage is associated with the formation of biofilms by Pseudomonas species https://www.biorxiv.org/content/10.1101/2025.07.11.664243v1
July 12, 2025 at 8:16 AM
Thrilled to see my first publication on food chain biofilms out! Check out the super cool biofilms Pseudomonas makes on food surfaces and the contribution of these to food spoilage. We're continuing to work in this space to find novel ways to extend food shelf life and reduce food waste! 🧫🧪
Reposted by Wee Boon Tan
Great news - this work is now published!! Big congrats to @yeowjiang.bsky.social - amazing that he mastered cryo-EM and now solved his 2nd set of strs! And kudos to Chee Geng (& Nadege) for non-trivial homogeneous purification of TolQRA!
pubs.acs.org/doi/10.1021/...
#MicroSky #SGBUG 1/4
pubs.acs.org/doi/10.1021/...
#MicroSky #SGBUG 1/4
July 2, 2025 at 5:58 AM
Great news - this work is now published!! Big congrats to @yeowjiang.bsky.social - amazing that he mastered cryo-EM and now solved his 2nd set of strs! And kudos to Chee Geng (& Nadege) for non-trivial homogeneous purification of TolQRA!
pubs.acs.org/doi/10.1021/...
#MicroSky #SGBUG 1/4
pubs.acs.org/doi/10.1021/...
#MicroSky #SGBUG 1/4
Reposted by Wee Boon Tan
@weeboont.bsky.social talked about our lab's recent preprint where we report AsmA superfamily proteins, believed to be phospholipid transporters, localizing to cell poles in E. coli. Really interesting observations, with impt implications for OM assembly!
Read it 👇!
www.biorxiv.org/content/10.1...
Read it 👇!
www.biorxiv.org/content/10.1...
June 13, 2025 at 10:23 AM
@weeboont.bsky.social talked about our lab's recent preprint where we report AsmA superfamily proteins, believed to be phospholipid transporters, localizing to cell poles in E. coli. Really interesting observations, with impt implications for OM assembly!
Read it 👇!
www.biorxiv.org/content/10.1...
Read it 👇!
www.biorxiv.org/content/10.1...
Reposted by Wee Boon Tan
Lipid interactions are important for the Tol-Pal complex in maintaining outer membrane lipid homeostasis https://www.biorxiv.org/content/10.1101/2025.06.10.658765v1
June 11, 2025 at 2:45 PM
Lipid interactions are important for the Tol-Pal complex in maintaining outer membrane lipid homeostasis https://www.biorxiv.org/content/10.1101/2025.06.10.658765v1
Reposted by Wee Boon Tan
Our new paper on FtsN is out! Now there are three tracks for organized cell wall constriction-- the old, boring E. coli always manages to surprise us.
A special shout-out to all the people who made this work possible, especially Dr. Jason Lyu, and Dr. David Weiss.
www.nature.com/articles/s41...
A special shout-out to all the people who made this work possible, especially Dr. Jason Lyu, and Dr. David Weiss.
www.nature.com/articles/s41...
Third track model for coordination of septal peptidoglycan synthesis and degradation by FtsN in Escherichia coli - Nature Microbiology
Partitioning of FtsN and the septal peptidoglycan synthesis complex between an active synthesis track and an inactive denuded glycan track coordinates opposing peptidoglycan synthesis and degradation ...
www.nature.com
May 27, 2025 at 5:13 PM
Our new paper on FtsN is out! Now there are three tracks for organized cell wall constriction-- the old, boring E. coli always manages to surprise us.
A special shout-out to all the people who made this work possible, especially Dr. Jason Lyu, and Dr. David Weiss.
www.nature.com/articles/s41...
A special shout-out to all the people who made this work possible, especially Dr. Jason Lyu, and Dr. David Weiss.
www.nature.com/articles/s41...
Reposted by Wee Boon Tan
Our latest work on the bacterial Tol-Pal complex now published! Kudos & congrats to the brilliant @weeboont.bsky.social! We can (finally) confidently assign the true function of Tol-Pal in maintaining OM lipid homeostasis, independent of cell division! 👇 1/7 #microsky
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Primary role of the Tol-Pal complex in bacterial outer membrane lipid homeostasis - Nature Communications
The Tol-Pal complex is important for multiple cellular processes in Gram-negative bacteria, but its molecular functions remain unclear. Here, Tan & Chng provide experimental evidence supporting th...
www.nature.com
April 3, 2025 at 7:09 AM
Our latest work on the bacterial Tol-Pal complex now published! Kudos & congrats to the brilliant @weeboont.bsky.social! We can (finally) confidently assign the true function of Tol-Pal in maintaining OM lipid homeostasis, independent of cell division! 👇 1/7 #microsky
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Wee Boon Tan
My lab at SCELSE, NTU, Singapore, is recruiting a PhD student to study food chain biofilms! The PhD will commence in January 2026 and tuition and stipend is provided for 4 years. Singaporean and international applicants welcome. See attached for more details. Please share widely!
February 17, 2025 at 2:44 AM
My lab at SCELSE, NTU, Singapore, is recruiting a PhD student to study food chain biofilms! The PhD will commence in January 2026 and tuition and stipend is provided for 4 years. Singaporean and international applicants welcome. See attached for more details. Please share widely!
Reposted by Wee Boon Tan
Really excited to be sharing our newest work on the bacterial Tol-Pal complex! The amazing Jiang YEOW solved cryo-EM structures of the TolQRA complex in 2 rotary states, aiding us to come up with a model for force transduction across the cell envelope! Pls RT! #SGBUG #SCELSE_SG #ChemNUS
Structural insights into the force-transducing mechanism of a motor-stator complex important for bacterial outer membrane lipid homeostasis https://www.biorxiv.org/content/10.1101/2024.11.27.625625v1
Structural insights into the force-transducing mechanism of a motor-stator complex important for bacterial outer membrane lipid homeostasis https://www.biorxiv.org/content/10.1101/2024.11.27.625625v1
Gram-negative bacteria assemble an asymmetric outer membrane (OM) that functions as an effective bar
www.biorxiv.org
November 30, 2024 at 2:35 AM
Really excited to be sharing our newest work on the bacterial Tol-Pal complex! The amazing Jiang YEOW solved cryo-EM structures of the TolQRA complex in 2 rotary states, aiding us to come up with a model for force transduction across the cell envelope! Pls RT! #SGBUG #SCELSE_SG #ChemNUS