Joe McKellar
@viroscope.bsky.social
Post-doc at @IGMM Montpellier
Studying Deltaviruses through the lens of a microscope 🔬
Studying Deltaviruses through the lens of a microscope 🔬
Thanks so much! That means so much to me 🤩
Working with MX1 proteins was definitely not easy, but coming across this mechanism felt super rewarding!
My question now is: what about the mechanisms behind the early stage blocks of the avian flus? 😄
Working with MX1 proteins was definitely not easy, but coming across this mechanism felt super rewarding!
My question now is: what about the mechanisms behind the early stage blocks of the avian flus? 😄
October 8, 2025 at 12:24 PM
Thanks so much! That means so much to me 🤩
Working with MX1 proteins was definitely not easy, but coming across this mechanism felt super rewarding!
My question now is: what about the mechanisms behind the early stage blocks of the avian flus? 😄
Working with MX1 proteins was definitely not easy, but coming across this mechanism felt super rewarding!
My question now is: what about the mechanisms behind the early stage blocks of the avian flus? 😄
Aha thank you for the compliment!
May 23, 2025 at 3:43 PM
Aha thank you for the compliment!
Thanks Maria! :)
It depends on the helper virus! With VSV and UGV the deltavirus free particles are productive, just much less so than the Trojan Horse particles (over 100-fold for UGV). However for HSV-1 they are totally unproductive, probably because they can't access/decorate with HSV-1 GPs!
It depends on the helper virus! With VSV and UGV the deltavirus free particles are productive, just much less so than the Trojan Horse particles (over 100-fold for UGV). However for HSV-1 they are totally unproductive, probably because they can't access/decorate with HSV-1 GPs!
May 10, 2025 at 11:48 AM
Thanks Maria! :)
It depends on the helper virus! With VSV and UGV the deltavirus free particles are productive, just much less so than the Trojan Horse particles (over 100-fold for UGV). However for HSV-1 they are totally unproductive, probably because they can't access/decorate with HSV-1 GPs!
It depends on the helper virus! With VSV and UGV the deltavirus free particles are productive, just much less so than the Trojan Horse particles (over 100-fold for UGV). However for HSV-1 they are totally unproductive, probably because they can't access/decorate with HSV-1 GPs!
Don't hesitate to get in touch if you'd like to know more!
May 10, 2025 at 8:12 AM
Don't hesitate to get in touch if you'd like to know more!
Special thanks to Aurélien Fouillen, who was there since the initial observations and who was essential for all the EM data of the paper, and of course @karimaj.bsky.social, who was extremely supportive in my pursual of what was originally an anecdotal, unexpected, observation!
May 10, 2025 at 8:12 AM
Special thanks to Aurélien Fouillen, who was there since the initial observations and who was essential for all the EM data of the paper, and of course @karimaj.bsky.social, who was extremely supportive in my pursual of what was originally an anecdotal, unexpected, observation!
I would like to thank everyone involved in this project, for their expertise, their ideas and their enthusiasm!
May 10, 2025 at 8:12 AM
I would like to thank everyone involved in this project, for their expertise, their ideas and their enthusiasm!
This opens up many questions, as to whether deltaviruses may be causative agents of disease in humans, associating with helper viruses of different origins.
May 10, 2025 at 8:12 AM
This opens up many questions, as to whether deltaviruses may be causative agents of disease in humans, associating with helper viruses of different origins.
To conclude, we evidence a novel mode of viral transmission – spread through a viral Trojan Horse.
May 10, 2025 at 8:12 AM
To conclude, we evidence a novel mode of viral transmission – spread through a viral Trojan Horse.
We show that snake deltavirus can use a reptarenavirus (UGV-1) as a viral Trojan Horse, being found inside of their particles, and that these Trojan Horse particles are over 100-fold more infectious than free deltavirus particles!
May 10, 2025 at 8:12 AM
We show that snake deltavirus can use a reptarenavirus (UGV-1) as a viral Trojan Horse, being found inside of their particles, and that these Trojan Horse particles are over 100-fold more infectious than free deltavirus particles!
Finally, the only animal deltavirus - helper virus association accepted to date is that of snake deltavirus and reptarenavirus, found in boa constrictors suffering from Boid Inclusion Body Disease.
May 10, 2025 at 8:12 AM
Finally, the only animal deltavirus - helper virus association accepted to date is that of snake deltavirus and reptarenavirus, found in boa constrictors suffering from Boid Inclusion Body Disease.
Furthermore, we show the tissue- and species-shifting capabilities that Trojan Horse particles allow, by showing infection of human neuronal cells with HSV-1 Trojan Horse particles!
Neuronal marker in 🟣 HSV-1 in 🟡, deltavirus in 🔵 and DNA in ⚪.
Neuronal marker in 🟣 HSV-1 in 🟡, deltavirus in 🔵 and DNA in ⚪.
May 10, 2025 at 8:12 AM
Furthermore, we show the tissue- and species-shifting capabilities that Trojan Horse particles allow, by showing infection of human neuronal cells with HSV-1 Trojan Horse particles!
Neuronal marker in 🟣 HSV-1 in 🟡, deltavirus in 🔵 and DNA in ⚪.
Neuronal marker in 🟣 HSV-1 in 🟡, deltavirus in 🔵 and DNA in ⚪.