Thanks Aidan! Very on-point comment, I appreciate it - and you are right. Often, one tries to simplify the system by limiting the number of variables for a single study - but in reality it seems more like an elaborate albeit stochastic ballet dance :)

We coupled in depth mutagenesis and biochemistry with high-resolution cryoEM, obtaining maps ranging from 2.37 to 2.9 Å.

This work was done by Ilana Nodelman (Bowman lab) and Heather Folkwein (Armache lab)

Thank you to everyone involved. It was a fun joint-venture!

Thank you🙏

This was a highly collaborative work between multiple labs, performed by a talented grad student in the Armache and Murakami labs, Natalie Smith. Thank you to all the authors for their incredible work!