Congrats Andreas!!

Thanks Max!!

@ijmonod.bsky.social @i2bcparissaclay.bsky.social @frm-officiel.bsky.social @EUR_G.E.N.E @upcite.bsky.social

This is part of my PhD work that I recently concluded in the lab of @sandraduharcourt.bsky.social 🎓 🎓
Huge thanks to everyone who contributed to this story (Mélanie, Marc & Valério, who are co-authors with me on this project !) as well as all collaborators for their dedication and insights. 🎉

By revealing how condensin I assists a domesticated transposase in sculpting the somatic genome, this work opens the door to exploring how genome organisation shapes DNA elimination in Paramecium 🤩

👉 To conclude, we show that condensin I physically and functionally interacts with PiggyMac, thus highlighting a strong interaction between the two machineries.

🧬 We demonstrate that this new development-specific complex is essential for DNA elimination and the establishment of H3K9me3 & H3K27me3 patterns during development, fully phenocopying PiggyMac depletion.

🧪Using TurboID proximity labelling, we identified condensin I subunits among the closest interactors of the PiggyMac endonuclease, leading to the identification of a development-specific condensin I complex

During Paramecium development, 30% of the germline genome, including TEs, is precisely eliminated to form a functional somatic genome.
The endonuclease PiggyMac and its partners catalyse this process, but how they are targeted to DNA is unknown. (see doi.org/10.1002/wrna... for more)

Congrats Raphael!! 🎉