🧭 #EndothelialCells are polarized against #BloodFlow direction ⤴️. #Centrioles are consistently upstream of the #nucleus. This polarity persists even in fixed tissue and could serve as a structural marker for flow direction. 📌
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In 🐁, some #pericyte #PrimaryCilia penetrate #astrocytic #endfeet and reach the #neuropil - sometimes near #synapses! Could these #cilia act as sensory “antennae” 📡for #NeurovascularCoupling?
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In 🐁🧠, #ultrastructure shows that #pericyte #cilia are often ensheathed by #astrocytic #endfeet. In 🚶‍♀️🧠, #astrocytes are swollen and cilia stay confined within the basal lamina. What are they sensing from the astrocytes?
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🗺️We mapped #MuralCells — including #pericytes and #smoothmuscle — as vectors➡️ to visualize where #PrimaryCilia🌟 occur across the 🐁🧠 #microvasculature.
The result? A striking pattern: #cilia are abundant on #capillaries and #venules, but almost absent on #arterioles.
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New on bioRxiv! 🚨
“Pericyte and Endothelial Primary Cilia and Centrioles have Disparate Organization Across the Brain Microvasculature”
This project studying #microvascular cells took an unexpected turn when we discovered that #pericytes have primary #cilia!
🔗 www.biorxiv.org/content/10.1...
🧪 🧵1/8

🎥 NAD 2025 Annual Meeting Highlights
From Oct 3–5, Neuroscience Academy Denmark hosted:
✅ 400+ participants
✅ 100 posters
✅ Keynotes on precision psychiatry, brain barriers, ultrasound treatments, brain stimulation, sleep & circadian rhythms

We look forward to next year!
#Neuroscience #NAD2025 🧪

Using #InVivo #VolumeImaging, we found #age related #vascular changes:
• ↑ #Tortuosity of #PenetratingArterioles
• ↓ #Arteriolar-capillary density
• ↑ #Capillaries at the venous end + #aneurysm-like bulbs downstream of #PrecapillarySphincters.

By recording #cortical #angiograms during i.v. injection of FITC-dextran 🟢 in #adult and #old #NG2DsRed mice, we time-colored the #arterioles 🔴 and #venules 🔵. We found that #OldMice have less #collaterals and more #tortuous #PenetratingArterioles.

We challenged mice with acute #BloodPressure spikes (~70% increase via angiotensin II). Result: both adult and #old mice got their #MyogenicResponse and #NeurovascularCoupling disrupted

To visualize this, we used #InVivo #TwoPhotonMicroscopy in anesthetized adult and aged mice. Watch this video showing PS dilation in response to “SensoryStimulation! 🔬👇

Here we show how 🧠 #precapillarysphincters react to #CorticalSpreadingDepolarization which is associated with #migraine with #aura 🤕. #CSD has 3 phases: constriction, dilation, and a long lasting constriction that interrupts #bloodflow🩸 to #capillaries (Tinyurl.com/euasfses).

Here is a #precapillarysphincter. As #arterioles 🟢 dive into the 🧠, they branch to smaller vessels. When larger arterioles (15-25um) branch into #capillaries, they need a #sphincter (3-4um) to reduce #RBC flow⚫️ and pressure in the #capillary. The resistance is provided by #muralcell🔴 encirclement.