Richard Goodman
@richard-goodman.bsky.social
| 📍Postdoctoral researcher at LSTM
| 🧬 Researching the evolution of antimicrobial resistance
| 🦠 Microbiology, Genomics, Bioinformatics
| 🧬 Researching the evolution of antimicrobial resistance
| 🦠 Microbiology, Genomics, Bioinformatics
We then used co-occurrence analysis on a larger dataset of short-read E. coli and K. pneumoniae genomes (n=772) which showed that the catB3∆443–633 (i.e. catB4 in figure) and catB3 genes nearly always co-occurred with aac(6')-Ib-cr, blaOXA-1 and the ESBL gene blaCTX-M-15.
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December 6, 2024 at 2:55 PM
We then used co-occurrence analysis on a larger dataset of short-read E. coli and K. pneumoniae genomes (n=772) which showed that the catB3∆443–633 (i.e. catB4 in figure) and catB3 genes nearly always co-occurred with aac(6')-Ib-cr, blaOXA-1 and the ESBL gene blaCTX-M-15.
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Another IS element, IS5, was found to insert into the promoter region of catA1, again knocking out its function. This shows how it is important to consider the gene environment along with the gene presence or absence in AMR gene detection and surveillance.
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December 6, 2024 at 2:55 PM
Another IS element, IS5, was found to insert into the promoter region of catA1, again knocking out its function. This shows how it is important to consider the gene environment along with the gene presence or absence in AMR gene detection and surveillance.
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This truncated catB3 gene (catB3∆443–633) appears as catB4 in some AMR gene databases, even though it doesn't confer phenotypic resistance this results in false positives and genotype-phenotype mismatches.
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December 6, 2024 at 2:55 PM
This truncated catB3 gene (catB3∆443–633) appears as catB4 in some AMR gene databases, even though it doesn't confer phenotypic resistance this results in false positives and genotype-phenotype mismatches.
3/7
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Using functional studies and genomics, we found that IS elements were part of the molecular mechanisms driving the rise in chloramphenicol susceptibility. IS26 was found to truncate the chloramphenicol resistance gene catB3, knocking out its function.
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December 6, 2024 at 2:55 PM
Using functional studies and genomics, we found that IS elements were part of the molecular mechanisms driving the rise in chloramphenicol susceptibility. IS26 was found to truncate the chloramphenicol resistance gene catB3, knocking out its function.
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