Ray Berkeley
@ray-berkeley.bsky.social
Postdoc @scripps.edu interested in protein-protein interactions and all things undruggable.
Reposted by Ray Berkeley
Very excited to share new work out today in @natchembio.nature.com on a new approach - FACES - for selectively imaging of phospholipids and other biomolecules at spatial resolutions down to individual membrane leaflets (1/n) www.nature.com/articles/s41...
Leaflet-specific phospholipid imaging using genetically encoded proximity sensors - Nature Chemical Biology
An approach combining bioorthogonal chemistry with genetically encoded fluorogen-activating proteins enables subcellular imaging of phospholipids and glycans, as well as the visualization of lipid tra...
www.nature.com
September 15, 2025 at 6:03 PM
Very excited to share new work out today in @natchembio.nature.com on a new approach - FACES - for selectively imaging of phospholipids and other biomolecules at spatial resolutions down to individual membrane leaflets (1/n) www.nature.com/articles/s41...
Reposted by Ray Berkeley
Thrilled to share this manuscript from my lab where we looked at how glycopolymers that compose the glycocalyx influence protein folding and protein-protein interactions via macromolecular crowding.
TLDR: The Glycocalyx stabilizes protein folding and PPIs
www.biorxiv.org/content/10.1...
TLDR: The Glycocalyx stabilizes protein folding and PPIs
www.biorxiv.org/content/10.1...
Glycopolymers stabilize protein folding and protein-protein interactions via enthalpic interactions
Macromolecular crowding is ubiquitous to physiological environments, perturbing the thermodynamics and kinetics of proteins via excluded volume and nonspecific chemical interactions. While crowding ha...
www.biorxiv.org
August 13, 2025 at 5:25 PM
Thrilled to share this manuscript from my lab where we looked at how glycopolymers that compose the glycocalyx influence protein folding and protein-protein interactions via macromolecular crowding.
TLDR: The Glycocalyx stabilizes protein folding and PPIs
www.biorxiv.org/content/10.1...
TLDR: The Glycocalyx stabilizes protein folding and PPIs
www.biorxiv.org/content/10.1...
Reposted by Ray Berkeley
Happy to share our contribution to this special issue dedicated to "Proximity-Induced Chemical Biology". We provide our take on targeted protein acetylation.
Nice work Wesley and Roy!
pubs.acs.org/doi/10.1021/...
Nice work Wesley and Roy!
pubs.acs.org/doi/10.1021/...
Targeted Protein Acetylation Through Chemically Induced Proximity
ConspectusProtein acetylation is a pervasive and reversible post-translational modification (PTM) that impacts various protein features including stability, localization, and interactions and regulate...
pubs.acs.org
August 13, 2025 at 10:32 PM
Happy to share our contribution to this special issue dedicated to "Proximity-Induced Chemical Biology". We provide our take on targeted protein acetylation.
Nice work Wesley and Roy!
pubs.acs.org/doi/10.1021/...
Nice work Wesley and Roy!
pubs.acs.org/doi/10.1021/...
Reposted by Ray Berkeley
New preprint from Daniel Du @fitzpatricklab.bsky.social shows #femtosecond light pulses can lens electron beams, promising higher contrast in #cryoET and better aberration correction in #cryoEM
tinyurl.com/257sw7j9
Experiments made possible by support from @cziscience.bsky.social #ImagingTheFuture
tinyurl.com/257sw7j9
Experiments made possible by support from @cziscience.bsky.social #ImagingTheFuture
June 17, 2025 at 3:38 PM
New preprint from Daniel Du @fitzpatricklab.bsky.social shows #femtosecond light pulses can lens electron beams, promising higher contrast in #cryoET and better aberration correction in #cryoEM
tinyurl.com/257sw7j9
Experiments made possible by support from @cziscience.bsky.social #ImagingTheFuture
tinyurl.com/257sw7j9
Experiments made possible by support from @cziscience.bsky.social #ImagingTheFuture
Reposted by Ray Berkeley
This could be a good time to mention we are looking for a new Lab Manager / Postdoc for the biochemistry lab with expertise protein expression, purification, and protein NMR. Details to follow.
January 21, 2025 at 3:16 PM
This could be a good time to mention we are looking for a new Lab Manager / Postdoc for the biochemistry lab with expertise protein expression, purification, and protein NMR. Details to follow.
Reposted by Ray Berkeley
Mass spec/proteomics chempostdoc in the Ferguson lab, UCSD, La Jolla, CA
chemsky 🧪 ⚗️
jobrxiv.org/job/universi...
chemsky 🧪 ⚗️
jobrxiv.org/job/universi...
Research Scientist - Mass spectrometry - Ferguson Lab
Post a job in 3min, or find thousands of job offers like this one at jobRxiv!
jobrxiv.org
January 16, 2025 at 1:34 AM
Mass spec/proteomics chempostdoc in the Ferguson lab, UCSD, La Jolla, CA
chemsky 🧪 ⚗️
jobrxiv.org/job/universi...
chemsky 🧪 ⚗️
jobrxiv.org/job/universi...
Reposted by Ray Berkeley
Nature research paper: Structural basis for the conformational protection of nitrogenase from O2
https://go.nature.com/4aiD344
https://go.nature.com/4aiD344
Structural basis for the conformational protection of nitrogenase from O2 - Nature
Cryo-electron microscopy of Azotobacter vinelandii FeSII–nitrogenase reveals a core complex of molybdenum–iron proteins (MoFePs), iron proteins (FePs) and FeSII, in which FeSII mediates interactions with MoFeP and FeP to position their FeS clusters in catalytically inactive but O2-protected states.
go.nature.com
January 13, 2025 at 8:04 PM
Nature research paper: Structural basis for the conformational protection of nitrogenase from O2
https://go.nature.com/4aiD344
https://go.nature.com/4aiD344
Awesome work from Sarah, @bcryo.bsky.social and friends in the Herzik Lab! It was cool to see this come together in such a short time.
1) Since I am the only Herzik lab member on @bsky.app, I get the privilege to announce our most recent publication from the Herzik/Tezcan labs. I got the wonderful opportunity to contribute on the cryoEM study of the oxygen protection mechansim employed by cells to protect Nitrogenase!
January 9, 2025 at 4:39 AM
Awesome work from Sarah, @bcryo.bsky.social and friends in the Herzik Lab! It was cool to see this come together in such a short time.
Awesome application of biorthogonal chemistry to trap non-ribosomal peptide synthetase complexes for cryo-EM, out yesterday from the Burkart Lab @ucsandiego.bsky.social
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Crosslinking intermodular condensation in non-ribosomal peptide biosynthesis - Nature
Structural studies of tyrocidine synthetase using site-selective crosslinking probes to link condensation domains with carrier protein substrates define key interactions and molecular mechanisms of no...
www.nature.com
December 12, 2024 at 7:23 PM
Awesome application of biorthogonal chemistry to trap non-ribosomal peptide synthetase complexes for cryo-EM, out yesterday from the Burkart Lab @ucsandiego.bsky.social
www.nature.com/articles/s41...
www.nature.com/articles/s41...
What do they keep in here…
December 4, 2024 at 8:52 AM
What do they keep in here…