Philip Ruthig
@philipruthig.bsky.social
Staff Scientist @erturklab.bsky.social, passionate neuroscientist, Light sheet microscopist, and Python aficionado.
Furthermore, we supply other scientists with the necessary functions that describe our experimental data - important for anyone who might want to simulate brain-wide activity!
August 21, 2025 at 12:39 PM
Furthermore, we supply other scientists with the necessary functions that describe our experimental data - important for anyone who might want to simulate brain-wide activity!
Based on this anatomical data, we estimate that corpus callosum fibers conduct signals ~25% faster than short white matter fibers, and show a higher portion of very fast fibers (>8m/s).
August 21, 2025 at 12:39 PM
Based on this anatomical data, we estimate that corpus callosum fibers conduct signals ~25% faster than short white matter fibers, and show a higher portion of very fast fibers (>8m/s).
In contrast, the g-ratio distribution of both sets of fibers is almost identical!
August 21, 2025 at 12:39 PM
In contrast, the g-ratio distribution of both sets of fibers is almost identical!
Additionally, both groups of fibers seems to have a large amount of fibers near the g-ratio of 0.6. This seems to be the equilibrium for long fibers where energy expenditure by the organism, space constraints, velocity and efficiency of signal transduction meet.
August 21, 2025 at 12:39 PM
Additionally, both groups of fibers seems to have a large amount of fibers near the g-ratio of 0.6. This seems to be the equilibrium for long fibers where energy expenditure by the organism, space constraints, velocity and efficiency of signal transduction meet.
We fitted a generalized extreme value function (GEV) to all of the distributions and found that both axon diameter and myelin thickness is downscaled in shorter fibers.
August 21, 2025 at 12:39 PM
We fitted a generalized extreme value function (GEV) to all of the distributions and found that both axon diameter and myelin thickness is downscaled in shorter fibers.
Using a fully automated deep learning analysis, we segmented each structure in the TEM data and analyzed the axonal diameter, myelin thickness, and g-ratio (ratio between axon radius & myelin thickness).
August 21, 2025 at 12:39 PM
Using a fully automated deep learning analysis, we segmented each structure in the TEM data and analyzed the axonal diameter, myelin thickness, and g-ratio (ratio between axon radius & myelin thickness).
To investigate this issue, we sampled white matter tissue from body donors from a variety of superficial white matter (mostly short fibers) and corpus callosum areas (very long fibers), and prepared them for transmission electron microscopy.
August 21, 2025 at 12:39 PM
To investigate this issue, we sampled white matter tissue from body donors from a variety of superficial white matter (mostly short fibers) and corpus callosum areas (very long fibers), and prepared them for transmission electron microscopy.
How are human brain regions connected? Are the connecting axons thicker and more myelinated the longer these connecting fibers are?
In our recent paper we find that indeed, they are! Read the skeetorial below ⬇️⬇️⬇️
plos.io/4mq5tOy
#neuroscience #neuroskyence #whitematter #deeplearning
In our recent paper we find that indeed, they are! Read the skeetorial below ⬇️⬇️⬇️
plos.io/4mq5tOy
#neuroscience #neuroskyence #whitematter #deeplearning
August 21, 2025 at 12:39 PM
How are human brain regions connected? Are the connecting axons thicker and more myelinated the longer these connecting fibers are?
In our recent paper we find that indeed, they are! Read the skeetorial below ⬇️⬇️⬇️
plos.io/4mq5tOy
#neuroscience #neuroskyence #whitematter #deeplearning
In our recent paper we find that indeed, they are! Read the skeetorial below ⬇️⬇️⬇️
plos.io/4mq5tOy
#neuroscience #neuroskyence #whitematter #deeplearning
I accidentally opened Edge today. Immediately, the browser spouts 'I am new and enhanced, try this AI-powered browsing'
After advertising these new groundbreaking features, Edge immediatly crashes and is unresponsive.
🙃
After advertising these new groundbreaking features, Edge immediatly crashes and is unresponsive.
🙃
February 25, 2025 at 8:11 PM
I accidentally opened Edge today. Immediately, the browser spouts 'I am new and enhanced, try this AI-powered browsing'
After advertising these new groundbreaking features, Edge immediatly crashes and is unresponsive.
🙃
After advertising these new groundbreaking features, Edge immediatly crashes and is unresponsive.
🙃
In contrast to our findings up to this point: When investigating neuronal cell bodies, we find that in the left and right auditory cortex, the structure is columnar (a,b), and very symmetrically so in the left and right auditory cortex (a,b,c).
January 20, 2025 at 3:59 PM
In contrast to our findings up to this point: When investigating neuronal cell bodies, we find that in the left and right auditory cortex, the structure is columnar (a,b), and very symmetrically so in the left and right auditory cortex (a,b,c).
Strikingly, we found that if we split the data by sex, the observed pattern is reversed! An important reminder to always include both sexes in studies - especially if a study concerns communication, or the neuronal basis of it!
January 20, 2025 at 3:59 PM
Strikingly, we found that if we split the data by sex, the observed pattern is reversed! An important reminder to always include both sexes in studies - especially if a study concerns communication, or the neuronal basis of it!
When splitting the data over different layers, cortical layer 2/3 seems to have the most prominent lateralized myelination, as opposed to L4 and L5. (🔵= Left, 🟡= Right )
January 20, 2025 at 3:59 PM
When splitting the data over different layers, cortical layer 2/3 seems to have the most prominent lateralized myelination, as opposed to L4 and L5. (🔵= Left, 🟡= Right )
In the auditory cortex, we find an asymmetry in the mean directionality of intracortical myelination (🔵= Left, 🟡= Right ). But what about specific layers?
Interestingly, in the neighbouring visual cortex (VC), this hemispheric asymmetry does not exist (or is not as pronounced)!
Interestingly, in the neighbouring visual cortex (VC), this hemispheric asymmetry does not exist (or is not as pronounced)!
January 20, 2025 at 3:59 PM
In the auditory cortex, we find an asymmetry in the mean directionality of intracortical myelination (🔵= Left, 🟡= Right ). But what about specific layers?
Interestingly, in the neighbouring visual cortex (VC), this hemispheric asymmetry does not exist (or is not as pronounced)!
Interestingly, in the neighbouring visual cortex (VC), this hemispheric asymmetry does not exist (or is not as pronounced)!
In our paper, we look at DISCO-cleared mouse brains and quantify the local directionality of myelinated axons in the auditory cortex, and look for asymmetries in neuronal cell body distributions as well. Here is a look at our light sheet microscopic raw data:
January 20, 2025 at 3:59 PM
In our paper, we look at DISCO-cleared mouse brains and quantify the local directionality of myelinated axons in the auditory cortex, and look for asymmetries in neuronal cell body distributions as well. Here is a look at our light sheet microscopic raw data:
The garden of intracortical connections of the auditory cortex in a cleared mouse brain - this is an image of intracortical myelin (🟡), Neurons (🔴), and nuclei (🔵).
This is a sneak peek of my PhD project, which is out in EJN soon - stay tuned :)
#Fluorescencefriday #neuroskyence
This is a sneak peek of my PhD project, which is out in EJN soon - stay tuned :)
#Fluorescencefriday #neuroskyence
January 10, 2025 at 12:59 PM
The garden of intracortical connections of the auditory cortex in a cleared mouse brain - this is an image of intracortical myelin (🟡), Neurons (🔴), and nuclei (🔵).
This is a sneak peek of my PhD project, which is out in EJN soon - stay tuned :)
#Fluorescencefriday #neuroskyence
This is a sneak peek of my PhD project, which is out in EJN soon - stay tuned :)
#Fluorescencefriday #neuroskyence
In our publication, we supply other scientists with the necessary functions that describe our experimental data - important for anyone who wants to simulate brain-wide activity!
November 17, 2024 at 9:33 AM
In our publication, we supply other scientists with the necessary functions that describe our experimental data - important for anyone who wants to simulate brain-wide activity!
On average, we estimate that corpus callosum fibers are ~33% faster than short white matter fibers, and show a dramatically higher portion of very fast fibers (>8m/s).
November 17, 2024 at 9:33 AM
On average, we estimate that corpus callosum fibers are ~33% faster than short white matter fibers, and show a dramatically higher portion of very fast fibers (>8m/s).
Looking at pooled data, the g-ratio distribution of superficial white matter is flatter than that of longer fibers - therefore, it is likely that different fibers require different combinations of axon radius and myelination thickness, also.
November 17, 2024 at 9:33 AM
Looking at pooled data, the g-ratio distribution of superficial white matter is flatter than that of longer fibers - therefore, it is likely that different fibers require different combinations of axon radius and myelination thickness, also.
Additionally, the group of longer fibers seems to have a larger amount of fibers near the g-ratio of 0.6. This seems to be the equilibrium for long fibers where energy expenditure by the organism, space constraints, velocity and efficiency of signal transduction meet.
November 17, 2024 at 9:33 AM
Additionally, the group of longer fibers seems to have a larger amount of fibers near the g-ratio of 0.6. This seems to be the equilibrium for long fibers where energy expenditure by the organism, space constraints, velocity and efficiency of signal transduction meet.
We fitted a generalized extreme value function (GEV) to all of the distributions and found that both axon diameter and myelin thickness is downscaled in shorter fibers.
November 17, 2024 at 9:33 AM
We fitted a generalized extreme value function (GEV) to all of the distributions and found that both axon diameter and myelin thickness is downscaled in shorter fibers.
Using a fully automated deep learning analysis, we segmented each structure in the TEM data and analyzed the axonal diameter, myelin thickness, and g-ratio (ratio between axon radius & myelin thickness).
November 17, 2024 at 9:33 AM
Using a fully automated deep learning analysis, we segmented each structure in the TEM data and analyzed the axonal diameter, myelin thickness, and g-ratio (ratio between axon radius & myelin thickness).
To investigate this issue, we sampled white matter tissue from body donors from a variety of superficial white matter (mostly short fibers) and corpus callosum areas (very long fibers), and prepared them for transmission electron microscopy.
November 17, 2024 at 9:33 AM
To investigate this issue, we sampled white matter tissue from body donors from a variety of superficial white matter (mostly short fibers) and corpus callosum areas (very long fibers), and prepared them for transmission electron microscopy.