Too excited not to share this beauty!
#fluorescenceFriday (but a day earlier)

TH staining in cyan
Virus injection in magenta

Zona Incerta is definitely a tough cookie 🍪 but getting there!

Day 5 at #wcbr2025 – don’t miss our panel!

Join us at 4:30 PM for a session fully dedicated to the medial #septum : inputs, outputs, and role in #motivation & #cognition. 🧠

Excited to share the stage with @smikulovic.bsky.social, @eliftuncozcan.bsky.social, and Dave Bortz. 👩‍🔬👨‍🔬

Day 3 at #wcbr2025 - lots of dopamine, acetylcholine, dorsal and ventral striatum, midbrain circuits and motivated behaviors. Learning so much from such a diverse group of scientists from single dendrite in vivo 2P imaging to high density recordings and modeling 🧠🤯 the field is moving so fast!

Kicked off the first day at #WCBR with an amazing plenary from Suzanne Dickson on #ghrelin, #hunger signaling and its effect on #hypothalamic and #midbrain circuits 🧠

Amazing science in an amazing venue! 🤩

Is #Fluorescence Tuesday a thing?

Still trying to improve the staining protocol, but love this pic of SN #neurons 🔬🧠🐭

3️⃣ Within the GABAergic population, we identified 6 subclusters in the continuum.

These include:
- PV+ and calretinin+ neurons, confirming prior observations
- NPAS1+/Sst+ neurons, which form a distinctive subpopulation
- Nts expressing neurons, adding to the complexity of the GABA population

4/4

2️⃣ ChAT-expressing neurons are better described along a transcriptomic continuum with two distinct endpoints:

- one predominantly expresses Galanin, a neuropeptide largely underexplored
-one co-expresses GABAergic machinery, aligning with the previously reported ChAT-GABA co-releasing neurons

3/n

Key findings:

1️⃣ Gad1 and Gad2, traditionally considered specific to GABAergic neurons, also show unexpected expression in ChAT and VGluT2-expressing neurons, challenging established assumptions about neuronal markers in the MSDB.

2/n

3️⃣ Within the GABAergic population, we identified 6 subclusters in the continuum.

These include:
- PV+ and calretinin+ neurons, confirming prior observations
- NPAS1+/Sst+ neurons, which form a distinctive subpopulation
- Nts expressing neurons, adding to the complexity of the GABA population

4/4

2️⃣ ChAT-expressing neurons are better described along a transcriptomic continuum with two distinct endpoints:

- one predominantly expresses Galanin, a neuropeptide largely underexplored
-one co-expresses GABAergic machinery, aligning with the previously reported ChAT-GABA co-releasing neurons

3/n

Key findings:

1️⃣ Gad1 and Gad2, traditionally considered specific to GABAergic neurons, also show unexpected expression in ChAT and VGluT2-expressing neurons, challenging established assumptions about neuronal markers in the MSDB.

The day has come!

I successfully defended my PhD thesis last week 🎓🇩🇪🎊

Thanks to my PhD supervisor, to the committee members, and to all the beautiful people that joined me in this incredible journey ❤️

Now, call me Dr. Mocellin!

First post on BlueSky! 💙

I finally took the time to read @neurograce.bsky.social's book and it was a real pleasure!

Amazingly written, it spans a century of neuroscience experiments and highlights how other fields have contributed to our understanding of the brain.

I really loved it!