Fascinating timelapses of #somite clock with cadherins from Kageyama Lab @dev-journal.bsky.social : journals.biologists.com/dev/article/...! A new player in clock and #fgf gradient cross-talk besides previously discovered Dusps linking both transcriptional and post-translationally. 🧪🔬 #devbio

4/5 We can further observe ERK signal dynamics with live translocation reporter (yellow), which shuttles into the nuclei (cyan) from cytoplasm at the moment MEK inhibitory drug is being perfused into the imaging chamber (~24min).

3/5 And as we perfuse 💉drugs 💊 and rinse them out in a pulsated fashion we can reconstitute somites in segmentation clock mutants (as I had done by hand in my postdoc @nature.com www.nature.com/articles/s41... ) now in a fully automated fashion, notice 5 boundaries forming at the end of movie 🐟:

2/5🐟 embryos can stably be imaged on stage in sub-cellular resolution:

1/5 Lab's first manuscript 🎉 is out @biorxivpreprint.bsky.social today! Beautifully, it is performed by a senior undergraduate thesis student, Mohammed! We developed a 3-D printed #zebrafish embryo imaging chamber 🔬and connected it to syringe pumps to be able to perfuse drugs in a timely fashion. 🧪