Thanks Joaquin!

5/5 All the 3-D printing files, and Arduino program are made available as supplementary files. Read for details: www.biorxiv.org/content/10.1...

4/5 We can further observe ERK signal dynamics with live translocation reporter (yellow), which shuttles into the nuclei (cyan) from cytoplasm at the moment MEK inhibitory drug is being perfused into the imaging chamber (~24min).

3/5 And as we perfuse 💉drugs 💊 and rinse them out in a pulsated fashion we can reconstitute somites in segmentation clock mutants (as I had done by hand in my postdoc @nature.com www.nature.com/articles/s41... ) now in a fully automated fashion, notice 5 boundaries forming at the end of movie 🐟:

2/5🐟 embryos can stably be imaged on stage in sub-cellular resolution:

Really cool experiments to solve a developmental mystery! 👏🏻I see how torque arises, but where does the chirality come from? Why are they whirling counter clockwise as seen from dorsal? Sorry didn't dig the preprint yet..

If you can combine with a controlled (in space and/or time) chemokine signal, would be interesting to see how to win the tug of war with optogenetic pulses...

I was seeing those in clouds frequently when doing drug induced somite experiments 🙃

The day which will come for every single one of us... It is interestingly different to live expecting it to come near while enjoying the legacy of what has been gifted and what awaits around the corner. With much respect and support. 🩵

To see supplementary figure S39 which I have no idea how ended up there go to page 109!