Oren Bachar
@orenbachar.bsky.social
PhD candidate, studying enzymatic and whole-cell nano-biohybrids. photocatalysis - nanomaterials - protein eng. - synthetic biology. Yehezkeli lab (Technion).
Reposted by Oren Bachar
📢📢📢 PhD Opportunity!
Join our research group at RUG! We're seeking a motivated PhD student for a project on biocatalytic cascade processes combining natural and designer enzymes, supported by machine learning.
Click below to learn more — and feel free to share!
sites.google.com/rug.nl/roelf...
Join our research group at RUG! We're seeking a motivated PhD student for a project on biocatalytic cascade processes combining natural and designer enzymes, supported by machine learning.
Click below to learn more — and feel free to share!
sites.google.com/rug.nl/roelf...
Roelfes group - Open Positions
PhD position on Hybrid biocatalytic cascades aided by Machine learning (1.0 FTE)
sites.google.com
May 12, 2025 at 9:10 AM
📢📢📢 PhD Opportunity!
Join our research group at RUG! We're seeking a motivated PhD student for a project on biocatalytic cascade processes combining natural and designer enzymes, supported by machine learning.
Click below to learn more — and feel free to share!
sites.google.com/rug.nl/roelf...
Join our research group at RUG! We're seeking a motivated PhD student for a project on biocatalytic cascade processes combining natural and designer enzymes, supported by machine learning.
Click below to learn more — and feel free to share!
sites.google.com/rug.nl/roelf...
What an amazing paper!
Using the molecular dye eosin Y as a photosensitizer to generate reducing equivalents in an engineered microbe. If that's not enough, they expressed a rhodopsin proton pump that generates ATP, enhancing the CBB cycle and CO2 conversion to acetoine.
doi.org/10.1016/j.jc...
Using the molecular dye eosin Y as a photosensitizer to generate reducing equivalents in an engineered microbe. If that's not enough, they expressed a rhodopsin proton pump that generates ATP, enhancing the CBB cycle and CO2 conversion to acetoine.
doi.org/10.1016/j.jc...
March 2, 2025 at 10:31 PM
What an amazing paper!
Using the molecular dye eosin Y as a photosensitizer to generate reducing equivalents in an engineered microbe. If that's not enough, they expressed a rhodopsin proton pump that generates ATP, enhancing the CBB cycle and CO2 conversion to acetoine.
doi.org/10.1016/j.jc...
Using the molecular dye eosin Y as a photosensitizer to generate reducing equivalents in an engineered microbe. If that's not enough, they expressed a rhodopsin proton pump that generates ATP, enhancing the CBB cycle and CO2 conversion to acetoine.
doi.org/10.1016/j.jc...
[2Fe-2S] clusters are responsible for so many amazing biocatalytic conversions and we always rely on their presence in host cells when designing cascades both in vitro and in vivo. Here's an interesting article that shines light on the "birth" of these clusters inside the cells.
Here the authors show that the assembly of [2Fe-2S] clusters by the ISC machinery proceeds by formation and fusion of [1Fe-1S] intermediates in the IscU scaffold protein
www.nature.com/articles/s41...
www.nature.com/articles/s41...
The ISC machinery assembles [2Fe–2S] clusters by formation and fusion of [1Fe–1S] precursors - Nature Chemical Biology
Iron–sulfur clusters are essential protein cofactors synthesized through a complex sequential process. Here the authors show that the assembly of [2Fe–2S] clusters by the iron–sulfur cluster assembly ...
www.nature.com
February 7, 2025 at 8:27 PM
[2Fe-2S] clusters are responsible for so many amazing biocatalytic conversions and we always rely on their presence in host cells when designing cascades both in vitro and in vivo. Here's an interesting article that shines light on the "birth" of these clusters inside the cells.
Inspiring stuff: using the power of biocondensates for creating distinctive environment in cells for new-to-nature biocatalysis performed by artificial metalloenzymes. Synthetic biology at its best!
A new paper reports a strategy to induce liquid-liquid phase separation in E. coli to enhance the performance of artificial metalloenzymes
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Artificial metalloenzyme assembly in cellular compartments for enhanced catalysis - Nature Chemical Biology
Artificial metalloenzymes (ArMs) often have sensitive metal centers. Here the authors enhance ArM performance by inducing liquid–liquid phase separation in Escherichia coli, creating protective compar...
www.nature.com
February 6, 2025 at 7:10 PM
Inspiring stuff: using the power of biocondensates for creating distinctive environment in cells for new-to-nature biocatalysis performed by artificial metalloenzymes. Synthetic biology at its best!
Reposted by Oren Bachar
By incorporating two cofactors into the four binding sites of streptavidin, we engineered programmable artifical enzymes for tandem abiotic transformations including an enantioselective formal C–H hydroxylation and a photooxidation-Michael addition. onlinelibrary.wiley.com/doi/10.1002/... #chemsky
January 7, 2025 at 9:32 AM
By incorporating two cofactors into the four binding sites of streptavidin, we engineered programmable artifical enzymes for tandem abiotic transformations including an enantioselective formal C–H hydroxylation and a photooxidation-Michael addition. onlinelibrary.wiley.com/doi/10.1002/... #chemsky
Reposted by Oren Bachar
Interesting paper by the @roelfesgroup.bsky.social and the group of Daniel Sauer in @angewandtechemie.bsky.social. They used genetic code expansion to incorporate a thiophenol-containing amino acid into a protein and used it to make an artificial gold enzyme. onlinelibrary.wiley.com/doi/10.1002/...
Artificial Gold Enzymes Using a Genetically Encoded Thiophenol‐Based Noble‐Metal‐Binding Ligand
Incorporating noble metals in artificial metalloenzymes (ArMs) is challenging due to the lack of suitable soft coordinating ligands among natural amino acids. We present a new class of ArMs featuring...
onlinelibrary.wiley.com
December 6, 2024 at 10:54 AM
Interesting paper by the @roelfesgroup.bsky.social and the group of Daniel Sauer in @angewandtechemie.bsky.social. They used genetic code expansion to incorporate a thiophenol-containing amino acid into a protein and used it to make an artificial gold enzyme. onlinelibrary.wiley.com/doi/10.1002/...