Nicholas McKenzie
@njmckenzie.bsky.social
Melbourne, AUS 🇦🇺
Bioinformatics enthusiast 💻🐍®️
PhD in biochem/mol bio 🧬
Hobbies: 3D printing and design 🖨️, music 🎵, & Japanese language 🇯🇵
Bioinformatics enthusiast 💻🐍®️
PhD in biochem/mol bio 🧬
Hobbies: 3D printing and design 🖨️, music 🎵, & Japanese language 🇯🇵
Reposted by Nicholas McKenzie
Save the CSIRO
Our national science & research agency is now facing more job cuts than under Tony Abbott
Call on the Albanese Govt to fund the scientists & research we need to tackle big issues we face from climate to food security and everything in between
www.davidpocock.com.au/save_our_csiro
Our national science & research agency is now facing more job cuts than under Tony Abbott
Call on the Albanese Govt to fund the scientists & research we need to tackle big issues we face from climate to food security and everything in between
www.davidpocock.com.au/save_our_csiro
November 19, 2025 at 9:49 AM
Save the CSIRO
Our national science & research agency is now facing more job cuts than under Tony Abbott
Call on the Albanese Govt to fund the scientists & research we need to tackle big issues we face from climate to food security and everything in between
www.davidpocock.com.au/save_our_csiro
Our national science & research agency is now facing more job cuts than under Tony Abbott
Call on the Albanese Govt to fund the scientists & research we need to tackle big issues we face from climate to food security and everything in between
www.davidpocock.com.au/save_our_csiro
Reposted by Nicholas McKenzie
Two alternative isoforms of PRC2 accessory subunit AEBP2 modulate developmental Polycomb functions in opposite ways - with broadly-expressed AEBP2L acting as an intrinsic inhibitor in somatic cells
@adrianbracken.bsky.social @davidovichlab.bsky.social and colleagues
www.embopress.org/doi/full/10....
@adrianbracken.bsky.social @davidovichlab.bsky.social and colleagues
www.embopress.org/doi/full/10....
November 4, 2025 at 12:51 PM
Two alternative isoforms of PRC2 accessory subunit AEBP2 modulate developmental Polycomb functions in opposite ways - with broadly-expressed AEBP2L acting as an intrinsic inhibitor in somatic cells
@adrianbracken.bsky.social @davidovichlab.bsky.social and colleagues
www.embopress.org/doi/full/10....
@adrianbracken.bsky.social @davidovichlab.bsky.social and colleagues
www.embopress.org/doi/full/10....
Reposted by Nicholas McKenzie
1/ It's long been assumed that AEBP2 recruit PRC2 to chromatin. Now, we show that AEBP2 usually does the exact opposite:
The only isoform of AEBP2 that is expressed in most cell types and tissues inhibits the chromatin-binding activity of PRC2. www.embopress.org/doi/full/10....
The only isoform of AEBP2 that is expressed in most cell types and tissues inhibits the chromatin-binding activity of PRC2. www.embopress.org/doi/full/10....
Auto-inhibition of PRC2 by the broadly expressed long isoform of AEBP2 | The EMBO Journal
imageimageAEBP2 is an accessory subunit of the PRC2 complex previously implicated both in promoting
and antagonizing Polycomb repressive activity. This study reveals that its alternative
isoforms, AEB...
www.embopress.org
October 31, 2025 at 10:42 PM
1/ It's long been assumed that AEBP2 recruit PRC2 to chromatin. Now, we show that AEBP2 usually does the exact opposite:
The only isoform of AEBP2 that is expressed in most cell types and tissues inhibits the chromatin-binding activity of PRC2. www.embopress.org/doi/full/10....
The only isoform of AEBP2 that is expressed in most cell types and tissues inhibits the chromatin-binding activity of PRC2. www.embopress.org/doi/full/10....
Thank you @adrianbracken.bsky.social for the collaboration 🤝, and this written summary of my latest co-first author paper to result from my PhD work under @davidovichlab.bsky.social. My congratulations and sincere thanks to all co-authors as well. This was a tremendous collective effort.
1/ 🚀 AEBP2 isn’t what we thought.
You were told that AEBP2 promotes PRC2 activity on chromatin.
We found the opposite: the most prevalent AEBP2 isoform inhibits PRC2 activity.
👉 surl.li/cgwqcq
A thread 🧵
You were told that AEBP2 promotes PRC2 activity on chromatin.
We found the opposite: the most prevalent AEBP2 isoform inhibits PRC2 activity.
👉 surl.li/cgwqcq
A thread 🧵
November 17, 2025 at 2:57 AM
Thank you @adrianbracken.bsky.social for the collaboration 🤝, and this written summary of my latest co-first author paper to result from my PhD work under @davidovichlab.bsky.social. My congratulations and sincere thanks to all co-authors as well. This was a tremendous collective effort.
Reposted by Nicholas McKenzie
Big thanks to @addgene.bsky.social for making our MoClo Baculo available as a kit! All the plasmids for Golden Gate assembly of baculovirus vectors, now bundled together:
www.addgene.org/kits/davidov...
🧪🧬 #MolecularCloning #baculovirus #ProteinExpression
www.addgene.org/kits/davidov...
🧪🧬 #MolecularCloning #baculovirus #ProteinExpression
Addgene: MoClo Baculo Toolkit
Use the MoClo Baculo Toolkit to efficiently generate multigene expression constructs for the baculovirus expression system.
www.addgene.org
May 15, 2025 at 12:55 AM
Big thanks to @addgene.bsky.social for making our MoClo Baculo available as a kit! All the plasmids for Golden Gate assembly of baculovirus vectors, now bundled together:
www.addgene.org/kits/davidov...
🧪🧬 #MolecularCloning #baculovirus #ProteinExpression
www.addgene.org/kits/davidov...
🧪🧬 #MolecularCloning #baculovirus #ProteinExpression
Reposted by Nicholas McKenzie
1/ H3K27me3 mimicry has repeatedly emerged through evolution, but what's the physiological relevance?
We show that JARID2 and PALI1 mimic H3K27me3 to antagonise PRC2 in vivo and restrict the spread of Polycomb domains.
🧵
www.biorxiv.org/content/10.1...
We show that JARID2 and PALI1 mimic H3K27me3 to antagonise PRC2 in vivo and restrict the spread of Polycomb domains.
🧵
www.biorxiv.org/content/10.1...
March 15, 2025 at 12:12 AM
1/ H3K27me3 mimicry has repeatedly emerged through evolution, but what's the physiological relevance?
We show that JARID2 and PALI1 mimic H3K27me3 to antagonise PRC2 in vivo and restrict the spread of Polycomb domains.
🧵
www.biorxiv.org/content/10.1...
We show that JARID2 and PALI1 mimic H3K27me3 to antagonise PRC2 in vivo and restrict the spread of Polycomb domains.
🧵
www.biorxiv.org/content/10.1...
Reposted by Nicholas McKenzie
We used cryoET and biophysical approaches in vitro together with molecular biology in differentiated cells to show that chromatin compacted by PRC1-CBX8 is porous and accessible while PRC1 is dynamic in it. 🧪🧬 #cryoET #Epigenetics #Polycomb www.nature.com/articles/s41...
Dynamic PRC1–CBX8 stabilizes a porous structure of chromatin condensates - Nature Structural & Molecular Biology
Here the authors show that a gene-inactivating protein complex packs inactive genes into a dynamic and accessible structure. The study challenges the traditional views that restricted accessibility an...
www.nature.com
January 16, 2025 at 9:24 PM
We used cryoET and biophysical approaches in vitro together with molecular biology in differentiated cells to show that chromatin compacted by PRC1-CBX8 is porous and accessible while PRC1 is dynamic in it. 🧪🧬 #cryoET #Epigenetics #Polycomb www.nature.com/articles/s41...
A PhD in Australia was financially hard enough when I started mine back in 2018. It would be unthinkable for me if I were hoping to start one now. www.abc.net.au/news/2025-01...
Alex loves doing her PhD, but says she's earning under the minimum wage
Alexandra Paton, who researches how to manage feral cat populations, says her PhD is important for Australia's wildlife but her income is lower than what she earned as a teenager at Macca's.
www.abc.net.au
January 17, 2025 at 12:27 AM
A PhD in Australia was financially hard enough when I started mine back in 2018. It would be unthinkable for me if I were hoping to start one now. www.abc.net.au/news/2025-01...