Nature Reviews Molecular Cell Biology, Published online: 27 November 2025; doi:10.1038/s41580-025-00934-0In this Comment, Na Sun explores how artificial intelligence-driven methodologies are poised to transform systems biology, particularly in the realm of tissue modelling, and outlines the key challenges that must be overcome to enable the development of truly predictive biological systems.

Nature Reviews Molecular Cell Biology, Published online: 27 November 2025; doi:10.1038/s41580-025-00921-5The centriole is crucial for fundamental cellular processes in eukaryotes, including cell polarity, signalling and motility. This Review discusses recent insights into the molecular architecture of centrioles and the principles governing their assembly, with a focus on human cells.

Nature Reviews Molecular Cell Biology, Published online: 27 November 2025; doi:10.1038/s41580-025-00935-zShiri Gur-Cohen discusses a 2007 paper from the Cotsarelis laboratory that reported that new hair follicles can arise de novo in response to wound-induced stress, indicating that in adulthood cells can assume new fate trajectories in response to microenvironmental pressures.

Nature Reviews Molecular Cell Biology, Published online: 26 November 2025; doi:10.1038/s41580-025-00909-1The endosomal sorting complex required for transport machinery supports key cellular functions including cell division, autophagy and membrane repair. This Review presents recent mechanistic and structural insights into the assembly and remodelling of endosomal sorting complex required for transport complexes in different cellular contexts.

Nature Reviews Molecular Cell Biology, Published online: 27 November 2025; doi:10.1038/s41580-025-00934-0In this Comment, Na Sun explores how artificial intelligence-driven methodologies are poised to transform systems biology, particularly in the realm of tissue modelling, and outlines the key challenges that must be overcome to enable the development of truly predictive biological systems.

Nature Reviews Molecular Cell Biology, Published online: 27 November 2025; doi:10.1038/s41580-025-00921-5The centriole is crucial for fundamental cellular processes in eukaryotes, including cell polarity, signalling and motility. This Review discusses recent insights into the molecular architecture of centrioles and the principles governing their assembly, with a focus on human cells.

Nature Reviews Molecular Cell Biology, Published online: 27 November 2025; doi:10.1038/s41580-025-00935-zShiri Gur-Cohen discusses a 2007 paper from the Cotsarelis laboratory that reported that new hair follicles can arise de novo in response to wound-induced stress, indicating that in adulthood cells can assume new fate trajectories in response to microenvironmental pressures.

Nature Reviews Molecular Cell Biology, Published online: 26 November 2025; doi:10.1038/s41580-025-00909-1The endosomal sorting complex required for transport machinery supports key cellular functions including cell division, autophagy and membrane repair. This Review presents recent mechanistic and structural insights into the assembly and remodelling of endosomal sorting complex required for transport complexes in different cellular contexts.

Nature Reviews Molecular Cell Biology, Published online: 20 November 2025; doi:10.1038/s41580-025-00897-2Biomolecular condensates are non-membrane-encapsulated compartments that control various biological processes. Recent studies have revealed that condensates change in response to stimuli and over time. This Review discusses the heterogeneity and composition changes of nuclear and cytoplasmic condensates, their regulation and how the changes affect cellular biochemical reactions.

Nature Reviews Molecular Cell Biology, Published online: 18 November 2025; doi:10.1038/s41580-025-00915-3Transcription-blocking DNA lesions lead to transcription stress that is associated with neurodegeneration and accelerated ageing. Recent structural and other findings have clarified how different obstructing lesions are recognized and removed and shed new light on the causes of various pathologies of repair deficiencies.

Nature Reviews Molecular Cell Biology, Published online: 20 November 2025; doi:10.1038/s41580-025-00897-2Biomolecular condensates are non-membrane-encapsulated compartments that control various biological processes. Recent studies have revealed that condensates change in response to stimuli and over time. This Review discusses the heterogeneity and composition changes of nuclear and cytoplasmic condensates, their regulation and how the changes affect cellular biochemical reactions.

Nature Reviews Molecular Cell Biology, Published online: 17 November 2025; doi:10.1038/s41580-025-00931-3In this Tools of the Trade article, Ding (Wang lab) describes the development of tissue expansion-enhanced mass-spectrometry imaging (TEMI), which combines an optimized tissue expansion method with mass spectrometry imaging to obtain multi-omic data at high spatial resolution and is compatible with expansion microscopy.

Nature Reviews Molecular Cell Biology, Published online: 18 November 2025; doi:10.1038/s41580-025-00915-3Transcription-blocking DNA lesions lead to transcription stress that is associated with neurodegeneration and accelerated ageing. Recent structural and other findings have clarified how different obstructing lesions are recognized and removed and shed new light on the causes of various pathologies of repair deficiencies.

Nature Reviews Molecular Cell Biology, Published online: 17 November 2025; doi:10.1038/s41580-025-00931-3In this Tools of the Trade article, Ding (Wang lab) describes the development of tissue expansion-enhanced mass-spectrometry imaging (TEMI), which combines an optimized tissue expansion method with mass spectrometry imaging to obtain multi-omic data at high spatial resolution and is compatible with expansion microscopy.

Nature Reviews Molecular Cell Biology, Published online: 12 November 2025; doi:10.1038/s41580-025-00928-yTimo Kohler highlights a study in which in vitro fertilization and cell culture techniques were adapted to generate chimeric blastocysts from northern and southern white rhinoceros species and establish embryonic stem cell lines — which has important implications for the conservation of endangered species and biodiversity.

Nature Reviews Molecular Cell Biology, Published online: 11 November 2025; doi:10.1038/s41580-025-00917-1The ability to track single cells in vivo, combined with insights into chromatin structure, immune–tissue crosstalk, bioelectric and metabolic cues and quantitative modelling, is changing our understanding of how tissues are repaired and renewed. This Review discusses how signals are regulated during regeneration and future directions for a more quantitative understanding of regenerative biology.

Nature Reviews Molecular Cell Biology, Published online: 11 November 2025; doi:10.1038/s41580-025-00930-4In this Tools of the Trade article, Zhang (Xiao Lab) highlights the development of a rotor-based fluorescent amino acid that acts as a sensor for crowded protein microenvironments, enabling researchers to monitor protein behaviour in vivo.

Nature Reviews Molecular Cell Biology, Published online: 10 November 2025; doi:10.1038/s41580-025-00907-3Protein and lipid cargoes are modified and sorted in the Golgi apparatus and packaged for delivery to diverse cellular destinations at the trans-Golgi network (TGN). This Review discusses recent insights into Golgi transport mechanisms and carrier biogenesis at the TGN.

Nature Reviews Molecular Cell Biology, Published online: 12 November 2025; doi:10.1038/s41580-025-00928-yTimo Kohler highlights a study in which in vitro fertilization and cell culture techniques were adapted to generate chimeric blastocysts from northern and southern white rhinoceros species and establish embryonic stem cell lines — which has important implications for the conservation of endangered species and biodiversity.

Nature Reviews Molecular Cell Biology, Published online: 11 November 2025; doi:10.1038/s41580-025-00917-1The ability to track single cells in vivo, combined with insights into chromatin structure, immune–tissue crosstalk, bioelectric and metabolic cues and quantitative modelling, is changing our understanding of how tissues are repaired and renewed. This Review discusses how signals are regulated during regeneration and future directions for a more quantitative understanding of regenerative biology.

Nature Reviews Molecular Cell Biology, Published online: 11 November 2025; doi:10.1038/s41580-025-00930-4In this Tools of the Trade article, Zhang (Xiao Lab) highlights the development of a rotor-based fluorescent amino acid that acts as a sensor for crowded protein microenvironments, enabling researchers to monitor protein behaviour in vivo.