Thomas Arnesen
@natmachinery.bsky.social
Prof. Univ. of Bergen & Helse Bergen | Basic & translational science | Molecular Biology | Cancer | Endocrinology | Rare diseases | Cytoskeleton | Ribosome | Protein biogenesis | PTMs | N-terminal acetylation
https://www.uib.no/en/rg/protein
https://www.uib.no/en/rg/protein
Developing leaves form a spatiotemporal oxygen gradient that is sensed through the oxygen sensing machinery and directs morphogenesis | New preprint from @daanweits.bsky.social and @syno2xis.bsky.social labs #N-degron #Cysteine #hypoxia @ispt-proteinterm.bsky.social www.biorxiv.org/content/10.1...
September 23, 2025 at 7:38 AM
Developing leaves form a spatiotemporal oxygen gradient that is sensed through the oxygen sensing machinery and directs morphogenesis | New preprint from @daanweits.bsky.social and @syno2xis.bsky.social labs #N-degron #Cysteine #hypoxia @ispt-proteinterm.bsky.social www.biorxiv.org/content/10.1...
Mechanism for voltage-gated channel inactivation uncovered | N-terminal acetylation of the intracellular domain of the Shaker potassium channel is key for plugging the pore | By Kenton Swartz lab in @nature.com @ispt-proteinterm.bsky.social www.nature.com/articles/s41...
August 7, 2025 at 8:11 AM
Mechanism for voltage-gated channel inactivation uncovered | N-terminal acetylation of the intracellular domain of the Shaker potassium channel is key for plugging the pore | By Kenton Swartz lab in @nature.com @ispt-proteinterm.bsky.social www.nature.com/articles/s41...
How to define degrons and E3 ligase specificities by peptide pulldowns | Methods in Enzymology | Kwon and Ji labs | #KCMF1 #Cysteine #Arg/N-degron @ispt-proteinterm.bsky.social www.sciencedirect.com/science/arti...
August 7, 2025 at 7:11 AM
How to define degrons and E3 ligase specificities by peptide pulldowns | Methods in Enzymology | Kwon and Ji labs | #KCMF1 #Cysteine #Arg/N-degron @ispt-proteinterm.bsky.social www.sciencedirect.com/science/arti...
Using a specific antibody to detect proteins carrying N-terminal formylation | Methods in Enzymology | Hwang lab @ispt-proteinterm.bsky.social www.sciencedirect.com/science/arti...
August 7, 2025 at 6:54 AM
Using a specific antibody to detect proteins carrying N-terminal formylation | Methods in Enzymology | Hwang lab @ispt-proteinterm.bsky.social www.sciencedirect.com/science/arti...
Assessing protein N-terminal myristoylation. Detailed protocols by Ed Tate's group in Methods in Enzymology. #lipidation @ispt-proteinterm.bsky.social @imperialcollegeldn.bsky.social www.sciencedirect.com/science/arti...
August 6, 2025 at 7:53 AM
Assessing protein N-terminal myristoylation. Detailed protocols by Ed Tate's group in Methods in Enzymology. #lipidation @ispt-proteinterm.bsky.social @imperialcollegeldn.bsky.social www.sciencedirect.com/science/arti...
Expression and purification of ribosome associated factors: Methionine aminopeptidases and N-terminal acetyltransferases | Methods in Enzymology | Marius Klein and Irmgard Sinning @uniheidelberg.bsky.social @ispt-proteinterm.bsky.social www.sciencedirect.com/science/arti...
July 29, 2025 at 7:46 AM
Expression and purification of ribosome associated factors: Methionine aminopeptidases and N-terminal acetyltransferases | Methods in Enzymology | Marius Klein and Irmgard Sinning @uniheidelberg.bsky.social @ispt-proteinterm.bsky.social www.sciencedirect.com/science/arti...
How to express, purify and assess the activity of the ATE1 arginyltransferase | Methods in Enzymology | Yi Zhang lab @ispt-proteinterm.bsky.social www.sciencedirect.com/science/arti...
July 29, 2025 at 7:38 AM
How to express, purify and assess the activity of the ATE1 arginyltransferase | Methods in Enzymology | Yi Zhang lab @ispt-proteinterm.bsky.social www.sciencedirect.com/science/arti...
Method to determine the identity and modification state of plastid precursor protein N-termini in the cytosol by combining protoplast protein import assays with targeted mass spectrometry | Methods in Enzymology | Baginsky lab. @ispt-proteinterm.bsky.social www.sciencedirect.com/science/arti...
July 29, 2025 at 7:30 AM
Method to determine the identity and modification state of plastid precursor protein N-termini in the cytosol by combining protoplast protein import assays with targeted mass spectrometry | Methods in Enzymology | Baginsky lab. @ispt-proteinterm.bsky.social www.sciencedirect.com/science/arti...
DEGRONOPEDIA - a web server for identifying and predicting protein degradation signals across eukaryotic proteomes. Detailed guide by Natalia Szulc @lumirare.com and Wojciech Pokrzywa @wpokrzywa.bsky.social in Methods in Enzymology. @ispt-proteinterm.bsky.social www.sciencedirect.com/science/arti...
July 9, 2025 at 8:32 AM
DEGRONOPEDIA - a web server for identifying and predicting protein degradation signals across eukaryotic proteomes. Detailed guide by Natalia Szulc @lumirare.com and Wojciech Pokrzywa @wpokrzywa.bsky.social in Methods in Enzymology. @ispt-proteinterm.bsky.social www.sciencedirect.com/science/arti...
How to use bacteria for arginylation assessment. Co-expression of ATE1 and substrate outlined by Yi Zhang and colleagues in Methods in Enzymology. @ispt-proteinterm.bsky.social www.sciencedirect.com/science/arti...
July 8, 2025 at 7:18 AM
How to use bacteria for arginylation assessment. Co-expression of ATE1 and substrate outlined by Yi Zhang and colleagues in Methods in Enzymology. @ispt-proteinterm.bsky.social www.sciencedirect.com/science/arti...
How to detect protein N-terminal arginylation - a key step of protein degradation via the Arg/N-degron pathway. By Jasper Eising and @ndissmeyer.bsky.social in Methods in Enzymology. @ispt-proteinterm.bsky.social www.sciencedirect.com/science/arti...
July 7, 2025 at 6:50 PM
How to detect protein N-terminal arginylation - a key step of protein degradation via the Arg/N-degron pathway. By Jasper Eising and @ndissmeyer.bsky.social in Methods in Enzymology. @ispt-proteinterm.bsky.social www.sciencedirect.com/science/arti...
How to assess protein stability and N-degrons via the Ubiquitin reference technique. From the Protein Metabolism Lab (Yong Tae Kwon) at SNU Seoul ups.snu.ac.kr in Methods in Enzymology @ispt-proteinterm.bsky.social www.sciencedirect.com/science/arti...
July 6, 2025 at 8:13 AM
How to assess protein stability and N-degrons via the Ubiquitin reference technique. From the Protein Metabolism Lab (Yong Tae Kwon) at SNU Seoul ups.snu.ac.kr in Methods in Enzymology @ispt-proteinterm.bsky.social www.sciencedirect.com/science/arti...
Acetylhistidine in our blood can prevent kidney disease and is produced by the HisAT (NAT16) enzyme displaying a rare double-GNAT structure. Great efforts by Matti Myllykoski and the rest of the team at @unibergen.bsky.social to get this published. #acetyltransferase www.nature.com/articles/s41...
July 1, 2025 at 1:44 PM
Acetylhistidine in our blood can prevent kidney disease and is produced by the HisAT (NAT16) enzyme displaying a rare double-GNAT structure. Great efforts by Matti Myllykoski and the rest of the team at @unibergen.bsky.social to get this published. #acetyltransferase www.nature.com/articles/s41...
Protein N-terminal modifications - Feature Review and cover in the recent issue of @cp-trendsbiochem.bsky.social Thanks to all authors, Editor @sanniejculbertson.bsky.social and reviewers for great work and support! @ispt-proteinterm.bsky.social www.cell.com/trends/bioch...
April 3, 2025 at 2:54 PM
Protein N-terminal modifications - Feature Review and cover in the recent issue of @cp-trendsbiochem.bsky.social Thanks to all authors, Editor @sanniejculbertson.bsky.social and reviewers for great work and support! @ispt-proteinterm.bsky.social www.cell.com/trends/bioch...
New approaches to treatment of Parkinson's disease? Investigation of All Disease-Relevant Lysine Acetylation Sites in
α-Synuclein Enabled by Non-canonical Amino Acid Mutagenesis. @upenn.edu
www.biorxiv.org/content/10.1...
α-Synuclein Enabled by Non-canonical Amino Acid Mutagenesis. @upenn.edu
www.biorxiv.org/content/10.1...
January 23, 2025 at 11:25 AM
New approaches to treatment of Parkinson's disease? Investigation of All Disease-Relevant Lysine Acetylation Sites in
α-Synuclein Enabled by Non-canonical Amino Acid Mutagenesis. @upenn.edu
www.biorxiv.org/content/10.1...
α-Synuclein Enabled by Non-canonical Amino Acid Mutagenesis. @upenn.edu
www.biorxiv.org/content/10.1...
A whole universe of protein N-terminal modifications and their biological impact! Thanks to all co-authors! @cp-trendsbiochem.bsky.social @ispt-proteinterm.bsky.social #oxidation #acetylation #methylation #myristoylation #arginylation #ubiquitylation www.sciencedirect.com/science/arti...
January 20, 2025 at 2:03 PM
A whole universe of protein N-terminal modifications and their biological impact! Thanks to all co-authors! @cp-trendsbiochem.bsky.social @ispt-proteinterm.bsky.social #oxidation #acetylation #methylation #myristoylation #arginylation #ubiquitylation www.sciencedirect.com/science/arti...
A majority of eukaryotic proteins are N-terminally acetylated. Here is an updated review of the responsible enzymes and the impact of this modification for proteins, cells and organisms @naturecomms.bsky.social @ispt-proteinterm.bsky.social #acetylation #N-termini #N-degron rdcu.be/d6mbd
January 15, 2025 at 4:33 PM
A majority of eukaryotic proteins are N-terminally acetylated. Here is an updated review of the responsible enzymes and the impact of this modification for proteins, cells and organisms @naturecomms.bsky.social @ispt-proteinterm.bsky.social #acetylation #N-termini #N-degron rdcu.be/d6mbd
The expanding landscape of canonical and non-canonical protein phosphorylation. New review out in TiBS @cellpress.bsky.social
www.cell.com/trends/bioch...
www.cell.com/trends/bioch...
December 4, 2024 at 4:50 PM
The expanding landscape of canonical and non-canonical protein phosphorylation. New review out in TiBS @cellpress.bsky.social
www.cell.com/trends/bioch...
www.cell.com/trends/bioch...
Methylarginine targeting chimeras for lysosomal degradation of intracellular proteins. The MrTAC chimera recruits PRMT1 to a target protein for induced degradation in lysosomes. @albrechtlab.bsky.social @natureportfolio.bsky.social www.nature.com/articles/s41...
December 4, 2024 at 8:06 AM
Methylarginine targeting chimeras for lysosomal degradation of intracellular proteins. The MrTAC chimera recruits PRMT1 to a target protein for induced degradation in lysosomes. @albrechtlab.bsky.social @natureportfolio.bsky.social www.nature.com/articles/s41...
p300/CBP-catalyzed acetylation safeguards minor zygotic genome activation via activating DUX.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
November 29, 2024 at 4:40 PM
p300/CBP-catalyzed acetylation safeguards minor zygotic genome activation via activating DUX.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Zebrafish model with defective actin N-terminal acetylation uncovers hearing defects. From the cover of Life Science Alliance @embopress.bsky.social - image from @varshneylab.social @sheng-jia.bsky.social #zebrafish #PTMs #acetylation #cytoskeleton
www.life-science-alliance.org/content/7/12...
www.life-science-alliance.org/content/7/12...
November 26, 2024 at 5:53 PM
Zebrafish model with defective actin N-terminal acetylation uncovers hearing defects. From the cover of Life Science Alliance @embopress.bsky.social - image from @varshneylab.social @sheng-jia.bsky.social #zebrafish #PTMs #acetylation #cytoskeleton
www.life-science-alliance.org/content/7/12...
www.life-science-alliance.org/content/7/12...
Four decades after the discovery of the first protein degradation signal, the degron, here is a new review on protein N-degron pathways from the master himself, Alexander Varshavsky. www.pnas.org/doi/10.1073/...
November 21, 2024 at 3:06 PM
Four decades after the discovery of the first protein degradation signal, the degron, here is a new review on protein N-degron pathways from the master himself, Alexander Varshavsky. www.pnas.org/doi/10.1073/...