Miguel Ramalho-Santos
@mrsantos.bsky.social
Scientist and Professor in Developmental Epigenetics.
Lunenfeld-Tanenbaum Research Institute
Dpt Molecular Genetics, University of Toronto, Canada
Lab: mrsantoslab.org
Coimbra, Portugal -> Cambridge, MA, USA -> S. Francisco, CA, USA -> Toronto, ON, Canada
Lunenfeld-Tanenbaum Research Institute
Dpt Molecular Genetics, University of Toronto, Canada
Lab: mrsantoslab.org
Coimbra, Portugal -> Cambridge, MA, USA -> S. Francisco, CA, USA -> Toronto, ON, Canada
Also noted: Single-cell nascent transcription reveals sparse genome usage and plasticity www.cell.com/cell/abstrac...
Single-cell nascent transcription reveals sparse genome usage and plasticity
scFLUENT-seq is a single-cell method that sensitively captures nascent nuclear transcriptomes
to reveal mRNA and ncRNA dynamics, heterogeneity across chromatin compartments, and
transcriptional divers...
www.cell.com
October 22, 2025 at 2:06 PM
Also noted: Single-cell nascent transcription reveals sparse genome usage and plasticity www.cell.com/cell/abstrac...
Also noted: Maternal stress triggers early-life eczema through fetal mast cell programming. www.nature.com/articles/s41...
Maternal stress triggers early-life eczema through fetal mast cell programming - Nature
Prenatal stress triggers molecular dysregulations in fetal neuroimmune circuits, leading to altered mast cell and sensory neuron function, which predisposes offspring to develop eczema in response to ...
www.nature.com
October 15, 2025 at 2:23 PM
Also noted: Maternal stress triggers early-life eczema through fetal mast cell programming. www.nature.com/articles/s41...
(4/4) Our findings redefine the role of TGF-β signaling in early development and suggest conserved parallels between embryonic diapause, stem cell quiescence, and cancer dormancy.
October 9, 2025 at 8:08 PM
(4/4) Our findings redefine the role of TGF-β signaling in early development and suggest conserved parallels between embryonic diapause, stem cell quiescence, and cancer dormancy.
(3/4) Mechanistically, Smad2 represses the metabolic regulator Pparg, preventing lipid accumulation that is incompatible with dormancy. This links signaling, metabolism, and chromatin control during developmental pausing.
October 9, 2025 at 8:08 PM
(3/4) Mechanistically, Smad2 represses the metabolic regulator Pparg, preventing lipid accumulation that is incompatible with dormancy. This links signaling, metabolism, and chromatin control during developmental pausing.
(2/4) We discovered that Nodal/Smad2 signaling, a branch of the TGF-β pathway, is essential to sustain this paused state in embryonic stem cells and embryos. www.biorxiv.org/content/10.1...
Nodal/Smad2 signaling sustains developmental pausing by repressing Pparg-mediated lipid metabolism
Cells and organisms can enter transient dormant states to survive unfavorable conditions during development, physiological adult contexts, and disease. One paradigmatic case of dormancy is diapause, w...
www.biorxiv.org
October 9, 2025 at 8:08 PM
(2/4) We discovered that Nodal/Smad2 signaling, a branch of the TGF-β pathway, is essential to sustain this paused state in embryonic stem cells and embryos. www.biorxiv.org/content/10.1...
(4/4) These findings provide new insights on the chromatin-level regulation of MYC-driven breast cancer and uncover CHD1 as a novel synthetic lethal vulnerability and potential therapeutic target.
September 4, 2025 at 7:11 PM
(4/4) These findings provide new insights on the chromatin-level regulation of MYC-driven breast cancer and uncover CHD1 as a novel synthetic lethal vulnerability and potential therapeutic target.
(3/4) We found that CHD1 is required to maintain an open chromatin landscape and a transcriptional program associated with cancer progression in MYC overexpressing breast cells and that this synthetic lethality may arise from nucleolar stress and p53 activation.
September 4, 2025 at 7:11 PM
(3/4) We found that CHD1 is required to maintain an open chromatin landscape and a transcriptional program associated with cancer progression in MYC overexpressing breast cells and that this synthetic lethality may arise from nucleolar stress and p53 activation.
(2/4) We found that knockdown of CHD1 in a xenograft model of MYC-driven breast cancer suppresses tumor growth in vivo. In tissue culture models, knockdown of CHD1 suppresses cell proliferation and induces cell death, specifically when MYC is overexpressed.
September 4, 2025 at 7:11 PM
(2/4) We found that knockdown of CHD1 in a xenograft model of MYC-driven breast cancer suppresses tumor growth in vivo. In tissue culture models, knockdown of CHD1 suppresses cell proliferation and induces cell death, specifically when MYC is overexpressed.
Also noted:
Pluripotency factor Tex10 finetunes Wnt signaling for spermatogenesis and primordial germ cell development. www.nature.com/articles/s41...
Pluripotency factor Tex10 finetunes Wnt signaling for spermatogenesis and primordial germ cell development. www.nature.com/articles/s41...
Pluripotency factor Tex10 finetunes Wnt signaling for spermatogenesis and primordial germ cell development - Nature Communications
Here they identify Tex10 as a critical regulator of male fertility and germ cell development. By fine-tuning Wnt signaling, Tex10 promotes spermatogenesis and PGC differentiation, offering potential t...
www.nature.com
August 12, 2025 at 2:41 PM
Also noted:
Pluripotency factor Tex10 finetunes Wnt signaling for spermatogenesis and primordial germ cell development. www.nature.com/articles/s41...
Pluripotency factor Tex10 finetunes Wnt signaling for spermatogenesis and primordial germ cell development. www.nature.com/articles/s41...
Also noted:
Nucleosome organization of mouse embryos during pre-implantation development.
www.nature.com/articles/s41...
Nucleosome organization of mouse embryos during pre-implantation development.
www.nature.com/articles/s41...
Nucleosome organization of mouse embryos during pre-implantation development - Scientific Reports
Scientific Reports - Nucleosome organization of mouse embryos during pre-implantation development
www.nature.com
July 22, 2025 at 4:34 PM
Also noted:
Nucleosome organization of mouse embryos during pre-implantation development.
www.nature.com/articles/s41...
Nucleosome organization of mouse embryos during pre-implantation development.
www.nature.com/articles/s41...
(3/3) We show that alpha satellite RNA is required for maintenance of the perinuclear compartment and for maintaining a low transcriptional output, including of ribosomal RNA, in human embryonic stem cells.
July 21, 2025 at 8:12 PM
(3/3) We show that alpha satellite RNA is required for maintenance of the perinuclear compartment and for maintaining a low transcriptional output, including of ribosomal RNA, in human embryonic stem cells.
(2/3) We found that alpha satellite RNA is highly chromatin-bound and localizes to the perinuclear compartment, a domain of the nucleus previously only thought to exist in cancer cells.
July 21, 2025 at 8:12 PM
(2/3) We found that alpha satellite RNA is highly chromatin-bound and localizes to the perinuclear compartment, a domain of the nucleus previously only thought to exist in cancer cells.