Miriam Lisci
@miriamlisci.bsky.social
EMBO postdoc fellow @jourdainlab.bsky.social at @dib-unil.bsky.social diving into the metabolism of cancer and immune cells | Previously @griffithslab.bsky.social @thecimr.bsky.social @Cambridge_Uni | City Coordinator of @pintofscience.ch
This work was performed @dib-unil.bsky.social thanks to the @snf-ch.bsky.social and an EMBO postdoctoral fellowship, and to the supports of all our colleagues @dib-unil.bsky.social!
#metabolism #cancer_metabolism #nutrient #screen #CRISPR #glutamine #metabolic_flexibility
#metabolism #cancer_metabolism #nutrient #screen #CRISPR #glutamine #metabolic_flexibility
November 20, 2024 at 1:58 PM
This work was performed @dib-unil.bsky.social thanks to the @snf-ch.bsky.social and an EMBO postdoctoral fellowship, and to the supports of all our colleagues @dib-unil.bsky.social!
#metabolism #cancer_metabolism #nutrient #screen #CRISPR #glutamine #metabolic_flexibility
#metabolism #cancer_metabolism #nutrient #screen #CRISPR #glutamine #metabolic_flexibility
We found that FBXW7 promotes growth in glutamine-limiting conditions by mediating pyruvate anaplerosis via pyruvate carboxylase (PC). This happens via a mechanism regulating MYC abundance->the MNT/SIN3A/HDAC network->epigenetic state of PC. Check out our preprint for more!
November 20, 2024 at 1:58 PM
We found that FBXW7 promotes growth in glutamine-limiting conditions by mediating pyruvate anaplerosis via pyruvate carboxylase (PC). This happens via a mechanism regulating MYC abundance->the MNT/SIN3A/HDAC network->epigenetic state of PC. Check out our preprint for more!
Interestingly, while some of these pathways had been previously investigated, the top hit of our genetic screen was a surprise: it was FBXW7, a tumor suppressor. Cells that were depleted of FBXW7 were particularly sensitive to glutamine depletion.
November 20, 2024 at 1:58 PM
Interestingly, while some of these pathways had been previously investigated, the top hit of our genetic screen was a surprise: it was FBXW7, a tumor suppressor. Cells that were depleted of FBXW7 were particularly sensitive to glutamine depletion.
These screens allowed us to build on ours and previous results to outline a recipe for proliferation in glutamine-depleted media: we find canonical and non-canonical nutrients (biotin!) as well as genes either required or detrimental for survival when glutamine is missing.
November 20, 2024 at 1:58 PM
These screens allowed us to build on ours and previous results to outline a recipe for proliferation in glutamine-depleted media: we find canonical and non-canonical nutrients (biotin!) as well as genes either required or detrimental for survival when glutamine is missing.
We employed a nutrient screen with >300 carbon and nitrogen sources, as well as a CRISPR/Cas9 screen, and asked what are the metabolites and the mechanisms promoting (or preventing!) cell growth upon glutamine restriction.
November 20, 2024 at 1:58 PM
We employed a nutrient screen with >300 carbon and nitrogen sources, as well as a CRISPR/Cas9 screen, and asked what are the metabolites and the mechanisms promoting (or preventing!) cell growth upon glutamine restriction.
We know that some cells (as cancer cells in the tumor microenvironment) are extremely resourceful when it comes to surviving nutrient deprivation. Yet, many of our cell lines struggle to proliferate when we keep them in culture in glutamine-limiting media. Why is that?
November 20, 2024 at 1:58 PM
We know that some cells (as cancer cells in the tumor microenvironment) are extremely resourceful when it comes to surviving nutrient deprivation. Yet, many of our cell lines struggle to proliferate when we keep them in culture in glutamine-limiting media. Why is that?
We employed a nutrient screen with >300 carbon and nitrogen sources, as well as a CRISPR/Cas9 screen, and asked what are the metabolites and the mechanisms promoting (or preventing!) cell growth upon glutamine restriction.
November 20, 2024 at 1:51 PM
We employed a nutrient screen with >300 carbon and nitrogen sources, as well as a CRISPR/Cas9 screen, and asked what are the metabolites and the mechanisms promoting (or preventing!) cell growth upon glutamine restriction.
We know that some cells (as cancer cells in the tumor microenvironment) are extremely resourceful when it comes to surviving nutrient deprivation. Yet, many of our cell lines struggle to proliferate when we keep them in culture in glutamine-limiting media. Why is that?
November 20, 2024 at 1:51 PM
We know that some cells (as cancer cells in the tumor microenvironment) are extremely resourceful when it comes to surviving nutrient deprivation. Yet, many of our cell lines struggle to proliferate when we keep them in culture in glutamine-limiting media. Why is that?