🔬 Funded by @NIH, this work contributes to the growing knowledge on intrinsically disordered proteins and their role in gene expression. Read more here:
www.cell.com/biophysrepor...

HIF-1α ensemble dimensions change less under osmotic stress than with specific mutations. In contrast, CITED2 shows significant ensemble changes under stress. This suggests cellular environments might naturally regulate AD function through changes in ensemble dimensions.

Mutations expanding the HIF-1α ensemble correlated with higher activity, while compaction correlated with reduced activity. For CITED2, ensemble size didn’t impact activity. Our findings suggest that ensemble dimensions may influence transcriptional activity in some ADs.

We studied two activation domains from HIF-1α and CITED2, using live-cell FRET microscopy to measure changes in their ensemble dimensions.

I’m grateful to Max Staller (@mvstaller.bsky.social) for his insights, discussions, and for providing the activity data for our correlations. Deep thanks to (blueskyless?) Aleah Camacho, Estefania Cuevas-Zepeda, Mary McCoy, and Feng Yu for their contributions throughout the project!

🔬 Funded by
@NIH, this work contributes to the growing knowledge on intrinsically disordered proteins and their role in gene expression. Read more here:
www.cell.com/biophysrepor...

HIF-1α ensemble dimensions change less under osmotic stress than with specific mutations. In contrast, CITED2 shows significant ensemble changes under stress. This suggests cellular environments might naturally regulate AD function through changes in ensemble dimensions.

Mutations expanding the HIF-1α ensemble correlated with higher activity, while compaction correlated with reduced activity. For CITED2, ensemble size didn’t impact activity. Our findings suggest that ensemble dimensions may influence transcriptional activity in some ADs.

We studied two activation domains from HIF-1α and CITED2, using live-cell FRET microscopy to measure changes in their ensemble dimensions.