HIF-1α ensemble dimensions change less under osmotic stress than with specific mutations. In contrast, CITED2 shows significant ensemble changes under stress. This suggests cellular environments might naturally regulate AD function through changes in ensemble dimensions.

Mutations expanding the HIF-1α ensemble correlated with higher activity, while compaction correlated with reduced activity. For CITED2, ensemble size didn’t impact activity. Our findings suggest that ensemble dimensions may influence transcriptional activity in some ADs.

We studied two activation domains from HIF-1α and CITED2, using live-cell FRET microscopy to measure changes in their ensemble dimensions.

📢 Excited to share our research with @shaharsu.bsky.social & @mvstaller.bsky.social on how activation domain (AD) structure affects gene expression! 🧬 TLDR: HIF-1α & CITED2 ADs suggest ensemble dimensions may regulate transcription in unique ways.
www.cell.com/biophysrepor...
🧵 Highlights:

HIF-1α ensemble dimensions change less under osmotic stress than with specific mutations. In contrast, CITED2 shows significant ensemble changes under stress. This suggests cellular environments might naturally regulate AD function through changes in ensemble dimensions.

Mutations expanding the HIF-1α ensemble correlated with higher activity, while compaction correlated with reduced activity. For CITED2, ensemble size didn’t impact activity. Our findings suggest that ensemble dimensions may influence transcriptional activity in some ADs.

We studied two activation domains from HIF-1α and CITED2, using live-cell FRET microscopy to measure changes in their ensemble dimensions.