Thank you, still some surprises left :)

Thanks!

Thanks Alexej and for your input!

Thanks Alexej! Indeed the puzzle pieces are fitting into place...so similar yet different at the same time.

Thanks Tomáš! Indeed I also think that's an exciting avenue.

Thank you! Indeed that seems to be the case in Chaetomium at least.

Many thanks to our colleagues @mpibp.bsky.social, @saumyakm.bsky.social, @hummerlab.bsky.social and Julian Langer, for their fantastic collaboration on this story!

Excitingly, we find that the SND3 translocon has an analogous organisation to the metazoan multipass translocon, indicating that fungi and other eukaryotic taxa have evolved a distinct, reduced-complexity machinery for the insertion of multipass membrane proteins.

Indeed, molecular dynamics simulations show that SND3 disrupts the lipid bilayer and promotes lipid scrambling via its membrane-embedded hydrophilic groove, suggesting that SND3 is a membrane insertase with a novel fold.

Within this Snd3 translocon complex, CCDC47 and the SEC61β N-terminus keep the SEC61 translocon ‘out of action’, indicating that substrate insertion proceeds via another route.

We reveal the cellular context of the SND3 component of the fungal SND pathway, showing it forms an intramembrane complex with CCDC47, the SEC61 translocon and TRAPɑ beneath the ribosome tunnel exit.

Hi Pietro! Good to see you here 😁