Mariya Mardamshina
@mari-mardamshin.bsky.social
Postdoctoral Fellow at Stanford University specializing in #spatialproteomics, #microscopy, deep visual #proteomics, advanced #image & #massspectrometry analysis. Passionate about unraveling cellular complexity through cutting-edge research and innovation🔬
Pinned
Multiplexed Deep Visual Proteomics Unveils Spatial Heterogeneity and Rare Endocrine States in Human Adult Pancreatic Islets
Pancreatic islets are highly specialized tissue compartments that regulate metabolism, and their dysfunction contributes to diseases such as prediabetes and diabetes. Characterizing islet morphology a...
www.biorxiv.org
So excited to share our preprint!
We developed multiplexed Deep Visual Proteomics(mxDVP) to deeply profile human pancreatic islets at spatial single-cell resolution, and identified rare endocrine states
#SpatialOmics #Proteomics #SingleCell #IsletBiology #OpenScience
www.biorxiv.org/content/10.1...
We developed multiplexed Deep Visual Proteomics(mxDVP) to deeply profile human pancreatic islets at spatial single-cell resolution, and identified rare endocrine states
#SpatialOmics #Proteomics #SingleCell #IsletBiology #OpenScience
www.biorxiv.org/content/10.1...
Reposted by Mariya Mardamshina
Great #iSCMS2025 poster by @mari-mardamshin.bsky.social, who presented mxDVP, integrating high-plex microscopy with ultra-sensitive MS to explore pancreatic islet heterogeneity at unprecedented resolution. Evosep One with Orbitrap Astral / timsTOF SCP, with Aurora Elite columns on both instruments.
September 29, 2025 at 6:07 AM
Great #iSCMS2025 poster by @mari-mardamshin.bsky.social, who presented mxDVP, integrating high-plex microscopy with ultra-sensitive MS to explore pancreatic islet heterogeneity at unprecedented resolution. Evosep One with Orbitrap Astral / timsTOF SCP, with Aurora Elite columns on both instruments.
Honored to be featured in the Researcher Spotlight by @ionopticks.bsky.social, sharing my path to spatial proteomics and our efforts to build pipelines that decode cellular complexity and uncover hidden states.
#massspectrometry #spatialbiogy #proteomics #singlecell #multiplexedimaging #dvp
#massspectrometry #spatialbiogy #proteomics #singlecell #multiplexedimaging #dvp
@mari-mardamshin.bsky.social has seen firsthand how the loss of pancreatic function can affect someone’s life.
“Knowing that our work might one day contribute to restoring this complex organ’s function brings an added layer of meaning to what we do in the lab each day.”
Learn more: bit.ly/46ddJdS
“Knowing that our work might one day contribute to restoring this complex organ’s function brings an added layer of meaning to what we do in the lab each day.”
Learn more: bit.ly/46ddJdS
From signals to stories: Dr. Mariya Mardamshina on decoding cells through spatial proteomic pipelines
Mariya Mardamshina M.D.
bit.ly
September 9, 2025 at 5:33 AM
Honored to be featured in the Researcher Spotlight by @ionopticks.bsky.social, sharing my path to spatial proteomics and our efforts to build pipelines that decode cellular complexity and uncover hidden states.
#massspectrometry #spatialbiogy #proteomics #singlecell #multiplexedimaging #dvp
#massspectrometry #spatialbiogy #proteomics #singlecell #multiplexedimaging #dvp
Reposted by Mariya Mardamshina
A portion of ye ole MS populace .. go.bsky.app/3XETWrh
June 6, 2025 at 12:57 PM
A portion of ye ole MS populace .. go.bsky.app/3XETWrh
June 5, 2025 at 8:51 PM
🎉 Excited to share our work at #ASMS2025!
Come visit Poster WP300 to learn about our Multiplexed DVP (MxDVP) workflow - a streamlined approach integrating #high-plex #imaging and ultra-sensitive #MS for spatially resolved cell states.
Let’s talk #spatialbiology and #single-cell depth! #teammassspec
Come visit Poster WP300 to learn about our Multiplexed DVP (MxDVP) workflow - a streamlined approach integrating #high-plex #imaging and ultra-sensitive #MS for spatially resolved cell states.
Let’s talk #spatialbiology and #single-cell depth! #teammassspec
June 4, 2025 at 1:23 PM
🎉 Excited to share our work at #ASMS2025!
Come visit Poster WP300 to learn about our Multiplexed DVP (MxDVP) workflow - a streamlined approach integrating #high-plex #imaging and ultra-sensitive #MS for spatially resolved cell states.
Let’s talk #spatialbiology and #single-cell depth! #teammassspec
Come visit Poster WP300 to learn about our Multiplexed DVP (MxDVP) workflow - a streamlined approach integrating #high-plex #imaging and ultra-sensitive #MS for spatially resolved cell states.
Let’s talk #spatialbiology and #single-cell depth! #teammassspec
Reposted by Mariya Mardamshina
Nano-scale, mega-impact: nanoPhos delivers 100× phosphoproteomics sensitivity, 70k sites from 1µg, 8k from 10ng! Powered by SPEC, it enables phosphoDVP: deep spatial phosphoproteomics from 1000 neurons, hitting 17k sites, a game changer for spatial omics.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
June 2, 2025 at 9:49 AM
Nano-scale, mega-impact: nanoPhos delivers 100× phosphoproteomics sensitivity, 70k sites from 1µg, 8k from 10ng! Powered by SPEC, it enables phosphoDVP: deep spatial phosphoproteomics from 1000 neurons, hitting 17k sites, a game changer for spatial omics.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
🌅 What a fantastic start to the day here in Baltimore! Joined the 5K charity run this morning: great company, perfect views, and all for a wonderful cause. Huge thanks to @sciex.bsky.social for organizing such an awesome event!
#ASMS2025 #SCIEXRun #teammassspec
#ASMS2025 #SCIEXRun #teammassspec
June 1, 2025 at 3:54 PM
🌅 What a fantastic start to the day here in Baltimore! Joined the 5K charity run this morning: great company, perfect views, and all for a wonderful cause. Huge thanks to @sciex.bsky.social for organizing such an awesome event!
#ASMS2025 #SCIEXRun #teammassspec
#ASMS2025 #SCIEXRun #teammassspec
On my way to Baltimore for #ASMS2025! Excited to reconnect and dive into cutting-edge mass spectrometry.
Any must-see sessions or cool poster recommendations from the #teammassspec community? Looking forward to your suggestions!
Any must-see sessions or cool poster recommendations from the #teammassspec community? Looking forward to your suggestions!
May 31, 2025 at 3:54 PM
On my way to Baltimore for #ASMS2025! Excited to reconnect and dive into cutting-edge mass spectrometry.
Any must-see sessions or cool poster recommendations from the #teammassspec community? Looking forward to your suggestions!
Any must-see sessions or cool poster recommendations from the #teammassspec community? Looking forward to your suggestions!
Excited to share our new preprint: PIPΣX — an open-source, no-code pipeline for automated multiplexed tissue image analysis. Works across platforms & organs.
Explore here: www.biorxiv.org/content/10.1...
Looking forward for your feedback!
#spatialbiology #opensource #multiplexedimaging #massspec
Explore here: www.biorxiv.org/content/10.1...
Looking forward for your feedback!
#spatialbiology #opensource #multiplexedimaging #massspec
Streamlining Multiplexed Tissue Image Analysis with PIPΣX: An Integrated Automated Pipeline for Image Processing and EXploration for Diverse Tissue Types
Spatial proteomics via multiplexed tissue imaging is transforming how we study biology, enabling researchers to investigate dozens of markers in a single tissue section and explore how cells behave in...
www.biorxiv.org
May 9, 2025 at 3:59 PM
Excited to share our new preprint: PIPΣX — an open-source, no-code pipeline for automated multiplexed tissue image analysis. Works across platforms & organs.
Explore here: www.biorxiv.org/content/10.1...
Looking forward for your feedback!
#spatialbiology #opensource #multiplexedimaging #massspec
Explore here: www.biorxiv.org/content/10.1...
Looking forward for your feedback!
#spatialbiology #opensource #multiplexedimaging #massspec
Reposted by Mariya Mardamshina
Excited to share our preprint on multiplexed DVP! mxDVP extends CODEX to DVP family, revealing spatial heterogeneity in human pancreatic islets with 12 endocrine sub-types including rare polyhormonal cells. Fantastic team effort #LundbergLab biorxiv.org/content/10.1101/2025.04.27.650857v1
Multiplexed Deep Visual Proteomics Unveils Spatial Heterogeneity and Rare Endocrine States in Human Adult Pancreatic Islets
Pancreatic islets are highly specialized tissue compartments that regulate metabolism, and their dysfunction contributes to diseases such as prediabetes and diabetes. Characterizing islet morphology a...
biorxiv.org
April 30, 2025 at 4:02 PM
Excited to share our preprint on multiplexed DVP! mxDVP extends CODEX to DVP family, revealing spatial heterogeneity in human pancreatic islets with 12 endocrine sub-types including rare polyhormonal cells. Fantastic team effort #LundbergLab biorxiv.org/content/10.1101/2025.04.27.650857v1
So excited to share our preprint!
We developed multiplexed Deep Visual Proteomics(mxDVP) to deeply profile human pancreatic islets at spatial single-cell resolution, and identified rare endocrine states
#SpatialOmics #Proteomics #SingleCell #IsletBiology #OpenScience
www.biorxiv.org/content/10.1...
We developed multiplexed Deep Visual Proteomics(mxDVP) to deeply profile human pancreatic islets at spatial single-cell resolution, and identified rare endocrine states
#SpatialOmics #Proteomics #SingleCell #IsletBiology #OpenScience
www.biorxiv.org/content/10.1...
Multiplexed Deep Visual Proteomics Unveils Spatial Heterogeneity and Rare Endocrine States in Human Adult Pancreatic Islets
Pancreatic islets are highly specialized tissue compartments that regulate metabolism, and their dysfunction contributes to diseases such as prediabetes and diabetes. Characterizing islet morphology a...
www.biorxiv.org
April 28, 2025 at 6:26 AM
So excited to share our preprint!
We developed multiplexed Deep Visual Proteomics(mxDVP) to deeply profile human pancreatic islets at spatial single-cell resolution, and identified rare endocrine states
#SpatialOmics #Proteomics #SingleCell #IsletBiology #OpenScience
www.biorxiv.org/content/10.1...
We developed multiplexed Deep Visual Proteomics(mxDVP) to deeply profile human pancreatic islets at spatial single-cell resolution, and identified rare endocrine states
#SpatialOmics #Proteomics #SingleCell #IsletBiology #OpenScience
www.biorxiv.org/content/10.1...
Reposted by Mariya Mardamshina
Have you already heard about the #ProteomicsMap ? Check it out and find out what great labs are out there, to get help in your projects, collaborate, and network! Check it out here 👉 eupa.org/map/ . Also, don't forget to register 🫵 your proteomics group group.bspr.org/group/register !
Proteomics Map - EuPA
Registration link:https://group.bspr.org/group/register
eupa.org
April 1, 2025 at 10:36 AM
Have you already heard about the #ProteomicsMap ? Check it out and find out what great labs are out there, to get help in your projects, collaborate, and network! Check it out here 👉 eupa.org/map/ . Also, don't forget to register 🫵 your proteomics group group.bspr.org/group/register !
Had the opportunity to join the the SAMBAI SPEAKS podcast, together with Dr. Roads, Dr. Olanipekun, and our patient advocate Lluvia Del Rio.
In this episode, we talked about work on the PROMINENT project, how we're exploring cancer development and why prevention deserves more attention in research.
In this episode, we talked about work on the PROMINENT project, how we're exploring cancer development and why prevention deserves more attention in research.
April 17, 2025 at 9:44 PM
Had the opportunity to join the the SAMBAI SPEAKS podcast, together with Dr. Roads, Dr. Olanipekun, and our patient advocate Lluvia Del Rio.
In this episode, we talked about work on the PROMINENT project, how we're exploring cancer development and why prevention deserves more attention in research.
In this episode, we talked about work on the PROMINENT project, how we're exploring cancer development and why prevention deserves more attention in research.
Reposted by Mariya Mardamshina
Our paper is out in Nature today! We use Deep Visual Proteomics (DVP) to dissect α1-antitrypsin deficiency (AATD) in human liver at single-cell resolution.
Let us take a tour through proteotoxic stress in intact human tissue — one hepatocyte at a time.
www.nature.com/articles/s41...
Let us take a tour through proteotoxic stress in intact human tissue — one hepatocyte at a time.
www.nature.com/articles/s41...
Deep Visual Proteomics maps proteotoxicity in a genetic liver disease - Nature
High-resolution spatial proteomics were used to map molecular events during hepatocyte stress in pseudotime across all fibrosis stages, recapitulating known disease progression markers and revealing e...
www.nature.com
April 16, 2025 at 3:16 PM
Our paper is out in Nature today! We use Deep Visual Proteomics (DVP) to dissect α1-antitrypsin deficiency (AATD) in human liver at single-cell resolution.
Let us take a tour through proteotoxic stress in intact human tissue — one hepatocyte at a time.
www.nature.com/articles/s41...
Let us take a tour through proteotoxic stress in intact human tissue — one hepatocyte at a time.
www.nature.com/articles/s41...
Reposted by Mariya Mardamshina
Out today in Nature Genetics: Using MS-based proteomics, we mapped 1,200+ plasma proteins in 2,100+ children, showing how genetics & development shape blood protein levels during childhood.
nature.com/articles/s41588-025-02089-2
#PediatricProteomics #pQTL
First author @liliniu.bsky.social explains ⬇️
nature.com/articles/s41588-025-02089-2
#PediatricProteomics #pQTL
First author @liliniu.bsky.social explains ⬇️
Plasma proteome variation and its genetic determinants in children and adolescents - Nature Genetics
This mass spectrometry-based proteomic study profiles the plasma proteome in 2,147 children and adolescents and reveals its association with age, sex, puberty, body mass index and genetics.
nature.com
February 19, 2025 at 10:44 AM
Out today in Nature Genetics: Using MS-based proteomics, we mapped 1,200+ plasma proteins in 2,100+ children, showing how genetics & development shape blood protein levels during childhood.
nature.com/articles/s41588-025-02089-2
#PediatricProteomics #pQTL
First author @liliniu.bsky.social explains ⬇️
nature.com/articles/s41588-025-02089-2
#PediatricProteomics #pQTL
First author @liliniu.bsky.social explains ⬇️
Reposted by Mariya Mardamshina
Reposted by Mariya Mardamshina
Our Deep Visual Proteomics workflow maps protein networks in bladder, prostate, seminal vesicle & lymph node Signet Ring Cell Carcinoma, revealing DNA damage response alterations & immune signatures that could guide therapy choice. Out now in NPJ Precision Oncology! rdcu.be/d8KyK
Deep visual proteomics reveals DNA replication stress as a hallmark of signet ring cell carcinoma
npj Precision Oncology - Deep visual proteomics reveals DNA replication stress as a hallmark of signet ring cell carcinoma
rdcu.be
February 6, 2025 at 8:06 AM
Our Deep Visual Proteomics workflow maps protein networks in bladder, prostate, seminal vesicle & lymph node Signet Ring Cell Carcinoma, revealing DNA damage response alterations & immune signatures that could guide therapy choice. Out now in NPJ Precision Oncology! rdcu.be/d8KyK
Reposted by Mariya Mardamshina
DIA-NN 2.0 is released! We consider it the biggest step forward in the history of DIA-NN. On modern LC-MS almost all identifications are now peptidoform-confident, with major improvements e.g. for phospho. Some other cool things too: github.com/vdemichev/Di...
Release DIA-NN 2.0 · vdemichev/DiaNN
We are excited to announce DIA-NN 2.0, the most significant milestone in the history of DIA-NN development.
Key Breakthroughs
Proteoform Confidence mode: DIA-NN 2.0 solves the long-standing chall...
github.com
January 29, 2025 at 9:04 AM
DIA-NN 2.0 is released! We consider it the biggest step forward in the history of DIA-NN. On modern LC-MS almost all identifications are now peptidoform-confident, with major improvements e.g. for phospho. Some other cool things too: github.com/vdemichev/Di...
Calling all #TeamMassSpec experts. I’m planning to revisit the classic SCX fractionation protocol but ran into a challenge - I can’t find a vendor for syringes with metal springs to make stage tips. I’d be so grateful for any suggestions or advice you might have 🙏
*attaching picture as an example
*attaching picture as an example
January 23, 2025 at 6:17 PM
Calling all #TeamMassSpec experts. I’m planning to revisit the classic SCX fractionation protocol but ran into a challenge - I can’t find a vendor for syringes with metal springs to make stage tips. I’d be so grateful for any suggestions or advice you might have 🙏
*attaching picture as an example
*attaching picture as an example
Reposted by Mariya Mardamshina
The research community has flocked to the social-media platform Bluesky.
Tell us about your experience
https://go.nature.com/42fihQG
Tell us about your experience
https://go.nature.com/42fihQG
Has Bluesky replaced X for scientists? Take Nature’s poll
The research community has flocked to the social-media platform Bluesky. Tell us about your experience.
go.nature.com
January 14, 2025 at 4:56 PM
The research community has flocked to the social-media platform Bluesky.
Tell us about your experience
https://go.nature.com/42fihQG
Tell us about your experience
https://go.nature.com/42fihQG
Spatial Proteomics was named as the method of the year 2024 🌟 #spatialproteomics #proteomics #teamscience #researchmethods
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Method of the Year 2024: spatial proteomics - Nature Methods
Approaches for profiling the spatial proteome in tissues are the basis of atlas-scale projects that are delivering on their promise for understanding biological complexity in health and disease.
www.nature.com
December 6, 2024 at 6:42 PM
Spatial Proteomics was named as the method of the year 2024 🌟 #spatialproteomics #proteomics #teamscience #researchmethods
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Mariya Mardamshina
That one is pretty much full, but I can add you both to the early career pack, we could use some company there 🙂
go.bsky.app/Dp8PHX1
go.bsky.app/Dp8PHX1
November 17, 2024 at 1:29 PM
That one is pretty much full, but I can add you both to the early career pack, we could use some company there 🙂
go.bsky.app/Dp8PHX1
go.bsky.app/Dp8PHX1
Reposted by Mariya Mardamshina
#teamMassSpec here is a starting pack in case you just moved over, or in case you've been absent for a while. This is #proteomics or #massspec related. The pack is not comprehensive but it's a start.
go.bsky.app/HH7kqEh
go.bsky.app/HH7kqEh
November 10, 2024 at 3:46 PM
#teamMassSpec here is a starting pack in case you just moved over, or in case you've been absent for a while. This is #proteomics or #massspec related. The pack is not comprehensive but it's a start.
go.bsky.app/HH7kqEh
go.bsky.app/HH7kqEh