Manuel Lera-Ramirez
@manu-lera.bsky.social
Working on OpenCloning, an Open Source web application to plan and document cloning.
https://opencloning.org/
Open Source in science enthusiast. Previously PomBase, yeast genetics, image analysis and cytoskeleton.
https://opencloning.org/
Open Source in science enthusiast. Previously PomBase, yeast genetics, image analysis and cytoskeleton.
✨ Last week at #ESSL2025 I presented a poster on #OpenCloning 🧬
🔥 Crowd favorite feature: pull gene sequences directly from NCBI — no copy-paste needed!
🎥 Here’s a quick demo
🔗 Visit opencloning.org
🔥 Crowd favorite feature: pull gene sequences directly from NCBI — no copy-paste needed!
🎥 Here’s a quick demo
🔗 Visit opencloning.org
September 22, 2025 at 10:46 AM
✨ Last week at #ESSL2025 I presented a poster on #OpenCloning 🧬
🔥 Crowd favorite feature: pull gene sequences directly from NCBI — no copy-paste needed!
🎥 Here’s a quick demo
🔗 Visit opencloning.org
🔥 Crowd favorite feature: pull gene sequences directly from NCBI — no copy-paste needed!
🎥 Here’s a quick demo
🔗 Visit opencloning.org
You can now ✅ verify your #OpenCloning sequences using sequencing data 🧬 (Sanger or Nanopore). Just load your files 📂 and see where they align! 🎯
#OpenCloning is free & Open Source, and supports most cloning methods! 🔬🛠️
👉 Try it here: opencloning.org
🎞️ Demo video: youtu.be/n0hedzvpW88?...
#OpenCloning is free & Open Source, and supports most cloning methods! 🔬🛠️
👉 Try it here: opencloning.org
🎞️ Demo video: youtu.be/n0hedzvpW88?...
April 1, 2025 at 1:53 PM
You can now ✅ verify your #OpenCloning sequences using sequencing data 🧬 (Sanger or Nanopore). Just load your files 📂 and see where they align! 🎯
#OpenCloning is free & Open Source, and supports most cloning methods! 🔬🛠️
👉 Try it here: opencloning.org
🎞️ Demo video: youtu.be/n0hedzvpW88?...
#OpenCloning is free & Open Source, and supports most cloning methods! 🔬🛠️
👉 Try it here: opencloning.org
🎞️ Demo video: youtu.be/n0hedzvpW88?...
#OpenCloning now displays primer details! 🧬🔍 Minimal info is shown in the table, and the full #primer3 report when clicking the ℹ️ icon.
🧪 The values in parenthesis are for the full primer, the preceding ones for the annealing part.
🔗 Try it yourself: opencloning.org
🎥 Demo: youtu.be/-wjOY4i6mjg
🧪 The values in parenthesis are for the full primer, the preceding ones for the annealing part.
🔗 Try it yourself: opencloning.org
🎥 Demo: youtu.be/-wjOY4i6mjg
March 27, 2025 at 4:32 PM
#OpenCloning now displays primer details! 🧬🔍 Minimal info is shown in the table, and the full #primer3 report when clicking the ℹ️ icon.
🧪 The values in parenthesis are for the full primer, the preceding ones for the annealing part.
🔗 Try it yourself: opencloning.org
🎥 Demo: youtu.be/-wjOY4i6mjg
🧪 The values in parenthesis are for the full primer, the preceding ones for the annealing part.
🔗 Try it yourself: opencloning.org
🎥 Demo: youtu.be/-wjOY4i6mjg
With #OpenCloning, you can very quickly access your locus of interest from the #NCBI to start cloning. No more downloading entire genomes and cutting your fragment of interest!
March 5, 2025 at 8:19 AM
With #OpenCloning, you can very quickly access your locus of interest from the #NCBI to start cloning. No more downloading entire genomes and cutting your fragment of interest!
🧬 Now you can access @sevaplasmids.bsky.social directly from #OpenCloning ✨.
🔗 try it: opencloning.org
🎞️ demo: youtu.be/n0hedzvpW88?...
🔗 try it: opencloning.org
🎞️ demo: youtu.be/n0hedzvpW88?...
February 17, 2025 at 11:23 AM
🧬 Now you can access @sevaplasmids.bsky.social directly from #OpenCloning ✨.
🔗 try it: opencloning.org
🎞️ demo: youtu.be/n0hedzvpW88?...
🔗 try it: opencloning.org
🎞️ demo: youtu.be/n0hedzvpW88?...
I have turned the primer designer of #OpenCloning into a stepper after feedback from lab members and @francescodb.bsky.social. It now conveys more clearly the workflow. Below an example of #primerDesign for #GatewayCloning
🔗 try it: opencloning.org
🎞️ demo: www.youtube.com/watch?v=n0he...
🔗 try it: opencloning.org
🎞️ demo: www.youtube.com/watch?v=n0he...
February 13, 2025 at 11:18 AM
I have turned the primer designer of #OpenCloning into a stepper after feedback from lab members and @francescodb.bsky.social. It now conveys more clearly the workflow. Below an example of #primerDesign for #GatewayCloning
🔗 try it: opencloning.org
🎞️ demo: www.youtube.com/watch?v=n0he...
🔗 try it: opencloning.org
🎞️ demo: www.youtube.com/watch?v=n0he...
🚀 #ShareYourCloning rebrands to #OpenCloning 🧫
An Open Source web application to plan and document molecular cloning 🧬 and genome engineering 🦠.
Latest version adds support for WekWikGene 🔍 as a plasmid source, and UI improvements.
👉 Check it out here: opencloning.org 🌍
An Open Source web application to plan and document molecular cloning 🧬 and genome engineering 🦠.
Latest version adds support for WekWikGene 🔍 as a plasmid source, and UI improvements.
👉 Check it out here: opencloning.org 🌍
February 6, 2025 at 1:29 PM
🚀 #ShareYourCloning rebrands to #OpenCloning 🧫
An Open Source web application to plan and document molecular cloning 🧬 and genome engineering 🦠.
Latest version adds support for WekWikGene 🔍 as a plasmid source, and UI improvements.
👉 Check it out here: opencloning.org 🌍
An Open Source web application to plan and document molecular cloning 🧬 and genome engineering 🦠.
Latest version adds support for WekWikGene 🔍 as a plasmid source, and UI improvements.
👉 Check it out here: opencloning.org 🌍
The new POMBOX kit is now available from @addgene.bsky.social
To plan your cloning with this and other Golden Gate kits, you can try the kit template in #ShareYourCloning
🔗 Visit shareyourcloning.org
👉 Go to Templates > Moclo
🎞️ Full demo video www.youtube.com/watch?v=n0he...
#pombe #MoClo
To plan your cloning with this and other Golden Gate kits, you can try the kit template in #ShareYourCloning
🔗 Visit shareyourcloning.org
👉 Go to Templates > Moclo
🎞️ Full demo video www.youtube.com/watch?v=n0he...
#pombe #MoClo
January 24, 2025 at 3:26 PM
The new POMBOX kit is now available from @addgene.bsky.social
To plan your cloning with this and other Golden Gate kits, you can try the kit template in #ShareYourCloning
🔗 Visit shareyourcloning.org
👉 Go to Templates > Moclo
🎞️ Full demo video www.youtube.com/watch?v=n0he...
#pombe #MoClo
To plan your cloning with this and other Golden Gate kits, you can try the kit template in #ShareYourCloning
🔗 Visit shareyourcloning.org
👉 Go to Templates > Moclo
🎞️ Full demo video www.youtube.com/watch?v=n0he...
#pombe #MoClo
New auto-annotate feature for #ShareYourCloning 🧬✨
Load your plasmid 🧫, auto-annotate it 🖋️, and start cloning! 🚀🔬
Built with #pLannotate by @matt-mcguffie.bsky.social 👏🙌
🔗 try it at shareyourcloning.org
Load your plasmid 🧫, auto-annotate it 🖋️, and start cloning! 🚀🔬
Built with #pLannotate by @matt-mcguffie.bsky.social 👏🙌
🔗 try it at shareyourcloning.org
November 26, 2024 at 9:30 PM
New auto-annotate feature for #ShareYourCloning 🧬✨
Load your plasmid 🧫, auto-annotate it 🖋️, and start cloning! 🚀🔬
Built with #pLannotate by @matt-mcguffie.bsky.social 👏🙌
🔗 try it at shareyourcloning.org
Load your plasmid 🧫, auto-annotate it 🖋️, and start cloning! 🚀🔬
Built with #pLannotate by @matt-mcguffie.bsky.social 👏🙌
🔗 try it at shareyourcloning.org