✨ Last week at #ESSL2025 I presented a poster on #OpenCloning 🧬

🔥 Crowd favorite feature: pull gene sequences directly from NCBI — no copy-paste needed!

🎥 Here’s a quick demo

🔗 Visit opencloning.org

🚀 OpenCloning now has a documentation page!

Whether you're getting started or diving deep, find everything you need to make the most of OpenCloning, including a powerful search function to quickly find exactly what you’re looking for.

#OpenCloning #OpenSource #SynBio

🔗 docs.opencloning.org

🚀 Just added support for Cre/Lox recombination to hashtag#OpenCloning! 🧬✨
👇 Check out an example showing excision of a plasmid from a locus and re-integration using classic LoxP sites.

👉 Try it here: opencloning.org
🎞️ Demo video: www.youtube.com/watch?v=n0he...

You can now ✅ verify your #OpenCloning sequences using sequencing data 🧬 (Sanger or Nanopore). Just load your files 📂 and see where they align! 🎯

#OpenCloning is free & Open Source, and supports most cloning methods! 🔬🛠️

👉 Try it here: opencloning.org
🎞️ Demo video: youtu.be/n0hedzvpW88?...

#OpenCloning now displays primer details! 🧬🔍 Minimal info is shown in the table, and the full #primer3 report when clicking the ℹ️ icon.

🧪 The values in parenthesis are for the full primer, the preceding ones for the annealing part.

🔗 Try it yourself: opencloning.org
🎥 Demo: youtu.be/-wjOY4i6mjg

With #OpenCloning, you can very quickly access your locus of interest from the #NCBI to start cloning. No more downloading entire genomes and cutting your fragment of interest!

🧬🔬 Ever wondered if there’s a #GateWay plasmid in @addgene.bsky.social with GFP and the att sites you need? 🤔✨ Wonder no more!

🚀 Visit GateWayMine to search a database of #AddGene plasmids by feature or site type! 🧪🔍

🔗 gatewaymine.netlify.app

More context in the thread! 🧵👇

🧬 Now you can access @sevaplasmids.bsky.social directly from #OpenCloning ✨.

🔗 try it: opencloning.org
🎞️ demo: youtu.be/n0hedzvpW88?...

I have turned the primer designer of #OpenCloning into a stepper after feedback from lab members and @francescodb.bsky.social. It now conveys more clearly the workflow. Below an example of #primerDesign for #GatewayCloning

🔗 try it: opencloning.org
🎞️ demo: www.youtube.com/watch?v=n0he...

🚀 #ShareYourCloning rebrands to #OpenCloning 🧫

An Open Source web application to plan and document molecular cloning 🧬 and genome engineering 🦠.

Latest version adds support for WekWikGene 🔍 as a plasmid source, and UI improvements.

👉 Check it out here: opencloning.org 🌍

The new POMBOX kit is now available from @addgene.bsky.social

To plan your cloning with this and other Golden Gate kits, you can try the kit template in #ShareYourCloning

🔗 Visit shareyourcloning.org
👉 Go to Templates > Moclo

🎞️ Full demo video www.youtube.com/watch?v=n0he...

#pombe #MoClo

Almost there with my 2024 resolutions! 99% #testCoverage of #ShareYourCloning

github.com/manulera/Sha...

New auto-annotate feature for #ShareYourCloning 🧬✨

Load your plasmid 🧫, auto-annotate it 🖋️, and start cloning! 🚀🔬

Built with #pLannotate by @matt-mcguffie.bsky.social 👏🙌

🔗 try it at shareyourcloning.org