Manuel Lera-Ramirez
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manu-lera.bsky.social
Manuel Lera-Ramirez
@manu-lera.bsky.social
Working on OpenCloning, an Open Source web application to plan and document cloning.

https://opencloning.org/

Open Source in science enthusiast. Previously PomBase, yeast genetics, image analysis and cytoskeleton.
😍🚀🚀🚀🚀
July 31, 2025 at 11:58 AM
Addgene: GoldenPiCS Kit
www.addgene.org
July 29, 2025 at 8:26 AM
🧪 Maybe it's time to give #OpenCloning a try!

✅ Free
🔓 Open Source
🧬 More cloning methods than SnapGene
👨‍🔬 Built by a fellow researcher — for researchers!

👉 Check it out: bsky.app/profile/manu...
#OpenCloning now displays primer details! 🧬🔍 Minimal info is shown in the table, and the full #primer3 report when clicking the ℹ️ icon.

🧪 The values in parenthesis are for the full primer, the preceding ones for the annealing part.

🔗 Try it yourself: opencloning.org
🎥 Demo: youtu.be/-wjOY4i6mjg
July 20, 2025 at 7:08 AM
👏👏👏
July 3, 2025 at 8:45 AM
Hi @retropz.bsky.social thanks! Happy to hear if you have any feedback, or if you would need some support for your users to get started.
May 19, 2025 at 12:47 PM
I built a database with mined gateway plasmids from addgene. Maybe it can be helpful.

bsky.app/profile/manu...
🧬🔬 Ever wondered if there’s a #GateWay plasmid in @addgene.bsky.social with GFP and the att sites you need? 🤔✨ Wonder no more!

🚀 Visit GateWayMine to search a database of #AddGene plasmids by feature or site type! 🧪🔍

🔗 gatewaymine.netlify.app

More context in the thread! 🧵👇
May 2, 2025 at 3:50 PM