9/9 If you want to learn more about the how we modified NIS selectivity, the lessons that we learn from its Cryo-EM structure, as well as read our proof-of-principle showing that this proposed therapy can work:

Check out our manuscript! 👇
www.biorxiv.org/content/10.1...

8/9 ✅My work details how we successfully engineered a version of human NIS that transports as much ReO₄⁻ as WT-NIS, and it doesn’t transport I⁻. We created a molecular tool that could be used to kill cancer cells while shielding the thyroid gland!

7/9 Fun fact! The protective non-radioactive I⁻ works just like the famous iodide pills given after a nuclear event like Chernobyl—it floods/saturates WT-NIS in the thyroid, protecting us from the radioactive I⁻ isotopes resulting from the disaster.

6/9 🛠️ An engineered-NIS molecule that only transports ReO₄⁻ opens up a new therapeutic strategy:

1. Introduce the engineered-NIS into tumor cells.

2. Treat with radioactive ReO₄⁻ to kill the tumoral cells.

3. Protect healthy thyroid by giving the patient non-radioactive I⁻! 🛡

5/9 💡 But what if we could be more specific? NIS doesn’t just transport I⁻; it can also transport other, “similar” molecules called oxyanions, like perrhenate (ReO₄⁻).

This led to our key question: could we engineer a version of NIS that only transports ReO₄⁻?

4/9🎯 For 30 years, Dr. Carrasco has dreamed of using NIS as a "trojan horse" to treat cancer.

The idea? Introduce NIS into non-thyroidal tumoral cells and give the patient radioactive iodide to kill them.

The problem? You'd also destroy the patient's healthy thyroid gland. ⚠️

3/9 🧬 The effectiveness of this treatment is all due to one protein: the Na+/I⁻ Symporter (NIS), the only transporter that mediates active transport of iodide (I⁻) into thyroid cells. The groundwork for understanding NIS was laid by my mentor, Dr. Nancy Carrasco, who first cloned its cDNA in 1996! 🙌

2/9 📈Did you know thyroid cancer has the highest 5-year survival rate? That's thanks to the highly effective radioiodide (¹³¹I⁻) therapy, a treatment that's been a game-changer since 1946!
seer.cancer.gov/statfacts/ht...

Congratulations, Oli and Huan! This is such hard work—I’m really excited to see it out now. I can’t wait to see if some kind of protein engineering can take advantage of the arrangement of the protomers’ C-termini.

9/x To read more, check out our preprint, first author @huanli00.bsky.social: www.biorxiv.org/content/10.1...