Lewis Holt
@lewisholt.bsky.social
PhD candidate in the Murphy-Royal astrocyte lab. Astrocytes in stress
🏴🇪🇺🇨🇭
🏴🇪🇺🇨🇭
Reposted by Lewis Holt
Thanks Bruno Geoffroy @crchum.bsky.social for the nice write up on our recent publication with @lewisholt.bsky.social
nouvelles.umontreal.ca/en/article/2...
nouvelles.umontreal.ca/en/article/2...
After early-life stress, astrocytes affect behaviour
Canadian researchers show that stress modifies the morphology of brain cells in mice, directly influencing the rodents’ level of physical activity.
nouvelles.umontreal.ca
September 4, 2025 at 1:55 PM
Thanks Bruno Geoffroy @crchum.bsky.social for the nice write up on our recent publication with @lewisholt.bsky.social
nouvelles.umontreal.ca/en/article/2...
nouvelles.umontreal.ca/en/article/2...
Super psyched to help close out the #AstrocyteCafe on thursday!
Join us this Thursday for the last #AstrocyteCafe session of this edition!!!
Looking forward to hearing the talks by Rowy Avishek,
@lewisholt.bsky.social and
@astrocytenet.bsky.social !😍😎
Register for our free mailing list or email us to get the link ;)
Looking forward to hearing the talks by Rowy Avishek,
@lewisholt.bsky.social and
@astrocytenet.bsky.social !😍😎
Register for our free mailing list or email us to get the link ;)
August 5, 2025 at 10:38 PM
Super psyched to help close out the #AstrocyteCafe on thursday!
Absolutely thrilled to share the first half of my PhD work! Super proud to see it finally out!
www.nature.com/articles/s41...
Thread below for a quick (10 point) recap of what we found!
🧵🧪🧠
www.nature.com/articles/s41...
Thread below for a quick (10 point) recap of what we found!
🧵🧪🧠
A divergent astrocytic response to stress alters activity patterns via distinct mechanisms in male and female mice - Nature Communications
Early-life stress has lifelong impacts on neuronal function and behavior. Here, the authors show that astrocytic glucocorticoid receptor signaling drives stress-induced neuronal and behavioral change ...
www.nature.com
July 11, 2025 at 12:23 PM
Absolutely thrilled to share the first half of my PhD work! Super proud to see it finally out!
www.nature.com/articles/s41...
Thread below for a quick (10 point) recap of what we found!
🧵🧪🧠
www.nature.com/articles/s41...
Thread below for a quick (10 point) recap of what we found!
🧵🧪🧠
Reposted by Lewis Holt
Final leg of the Tour de France done with an excellent @gliameeting.bsky.social !
Amazing science in a great city, (despite some smoke!) and a nice travel award for @lewisholt.bsky.social 🥳
Going to make sure we come back to this meeting again next time! #glia2025
Amazing science in a great city, (despite some smoke!) and a nice travel award for @lewisholt.bsky.social 🥳
Going to make sure we come back to this meeting again next time! #glia2025
July 11, 2025 at 8:26 AM
Final leg of the Tour de France done with an excellent @gliameeting.bsky.social !
Amazing science in a great city, (despite some smoke!) and a nice travel award for @lewisholt.bsky.social 🥳
Going to make sure we come back to this meeting again next time! #glia2025
Amazing science in a great city, (despite some smoke!) and a nice travel award for @lewisholt.bsky.social 🥳
Going to make sure we come back to this meeting again next time! #glia2025
Was great to present at @canglia.bsky.social today!
Cool to see all the glial research happening across Canada!
Cool to see all the glial research happening across Canada!
@lewisholt.bsky.social sharing some cool data about astrocytes driving stress-induced behaviour! #astroglia
May 20, 2025 at 6:19 PM
Was great to present at @canglia.bsky.social today!
Cool to see all the glial research happening across Canada!
Cool to see all the glial research happening across Canada!
Reposted by Lewis Holt
Excited to share that the astrocyte-centered half of my thesis work is now out @science.org ! With @mishaahrens.bsky.social and @marcduque.bsky.social
science.org/doi/10.1126/... 1/8
science.org/doi/10.1126/... 1/8
Norepinephrine changes behavioral state through astroglial purinergic signaling
Both neurons and glia communicate through diffusible neuromodulators; however, how neuron-glial interactions in such neuromodulatory networks influence circuit computation and behavior is unclear. Dur...
science.org
May 16, 2025 at 4:20 AM
Excited to share that the astrocyte-centered half of my thesis work is now out @science.org ! With @mishaahrens.bsky.social and @marcduque.bsky.social
science.org/doi/10.1126/... 1/8
science.org/doi/10.1126/... 1/8
Reposted by Lewis Holt
Thought this would be a nice way to enter the bsky community👇Delighted for Katie (now in Chris Dulla's lab at Tufts). Thank you to our collaborators, to the reviewers and to Cagla Eroglu for the highlight!
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Norepinephrine signals through astrocytes to modulate synapses
Locus ceruleus (LC)–derived norepinephrine (NE) drives network and behavioral adaptations to environmental saliencies by reconfiguring circuit functional connectivity, but the underlying synapse-level...
www.science.org
May 16, 2025 at 4:34 AM
Thought this would be a nice way to enter the bsky community👇Delighted for Katie (now in Chris Dulla's lab at Tufts). Thank you to our collaborators, to the reviewers and to Cagla Eroglu for the highlight!
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Super cool that these are finally out!! Check out some of the protocols we've been using to investigate the role of astrocytes in stress!
Very happy to share several common protocols used in our lab for the second edition of the book "Astrocytes: Methods and Protocols"
@lewisholt.bsky.social @astroantho.bsky.social
Thanks @dibenedetto-lab.bsky.social for being a very patient editor!
link.springer.com/book/10.1007...
@lewisholt.bsky.social @astroantho.bsky.social
Thanks @dibenedetto-lab.bsky.social for being a very patient editor!
link.springer.com/book/10.1007...
Astrocytes
This book gathers methods essential for investigating the multifaceted roles of astrocytes in both physiology and pathological conditions.
link.springer.com
March 21, 2025 at 5:40 PM
Super cool that these are finally out!! Check out some of the protocols we've been using to investigate the role of astrocytes in stress!
Multinucleated astrocytes always look so cool! #FluorescenceFriday
March 21, 2025 at 4:29 PM
Multinucleated astrocytes always look so cool! #FluorescenceFriday
Diversifying my portfolio with some Iba-1 staining. #fluorescentfriday
February 21, 2025 at 4:21 PM
Diversifying my portfolio with some Iba-1 staining. #fluorescentfriday
Reposted by Lewis Holt
Version of Record of our work at @elife.bsky.social has been published! Super proud of this one after all the hard work of the lab seeing it to the end.
Make sure to click through figure supplements as there is so much good stuff in there - sex differences, etc.
elifesciences.org/articles/99988
Make sure to click through figure supplements as there is so much good stuff in there - sex differences, etc.
elifesciences.org/articles/99988
Early-life stress induces persistent astrocyte dysfunction associated with fear generalisation
Amygdala astrocytes play a critical role in mediating the effects of stress during infancy on neuronal excitability, synaptic plasticity, and memory.
elifesciences.org
February 5, 2025 at 4:13 PM
Version of Record of our work at @elife.bsky.social has been published! Super proud of this one after all the hard work of the lab seeing it to the end.
Make sure to click through figure supplements as there is so much good stuff in there - sex differences, etc.
elifesciences.org/articles/99988
Make sure to click through figure supplements as there is so much good stuff in there - sex differences, etc.
elifesciences.org/articles/99988
technical question. Can anyone recommend a good commercially available SOX9 antibody for fixed tissue IHC? #astrosky #astrocytes
January 14, 2025 at 9:59 PM
technical question. Can anyone recommend a good commercially available SOX9 antibody for fixed tissue IHC? #astrosky #astrocytes
Reposted by Lewis Holt
What a year! #DLC3🔥 is up tested by many of you (👀 watch out for a paper in 2025), we hit 750K downloads, and have over 120 contributors to the code! @deeplabcut.bsky.social
❄️⛷just dropped: new human pose models & high perf. models!
❄️⛷just dropped: new human pose models & high perf. models!
December 20, 2024 at 10:08 PM
What a year! #DLC3🔥 is up tested by many of you (👀 watch out for a paper in 2025), we hit 750K downloads, and have over 120 contributors to the code! @deeplabcut.bsky.social
❄️⛷just dropped: new human pose models & high perf. models!
❄️⛷just dropped: new human pose models & high perf. models!
Reposted by Lewis Holt
Delighted to share an update to our preprint, reviewed at @elife.bsky.social, and now sent back for re-review.
Colossal work by the team and driven home by @mathiasgua.bsky.social
Quick 🧵 of what's new -
www.biorxiv.org/content/10.1...
Colossal work by the team and driven home by @mathiasgua.bsky.social
Quick 🧵 of what's new -
www.biorxiv.org/content/10.1...
Early-life stress induces persistent astrocyte dysfunction associated with fear generalisation
Early-life stress can have lifelong consequences, enhancing stress susceptibility and resulting in behavioural and cognitive deficits. While the effects of early-life stress on neuronal function have ...
www.biorxiv.org
December 9, 2024 at 11:19 PM
Delighted to share an update to our preprint, reviewed at @elife.bsky.social, and now sent back for re-review.
Colossal work by the team and driven home by @mathiasgua.bsky.social
Quick 🧵 of what's new -
www.biorxiv.org/content/10.1...
Colossal work by the team and driven home by @mathiasgua.bsky.social
Quick 🧵 of what's new -
www.biorxiv.org/content/10.1...
Reposted by Lewis Holt
Our latest review-with @bonniee-leee.bsky.social where we pour our ❤️🧠 & soul to outline our position on why we are so passionate about harnessing the power of sex differences & within sex diff to propel precision medicine forward let us know if we convince you www.nature.com/articles/s41...
Leveraging research into sex differences and steroid hormones to improve brain health - Nature Reviews Endocrinology
This Review outlines how the effects of steroid hormones on hippocampal neuroplasticity across the adult lifespan differ between the sexes, highlighting the importance of considering sex and steroid h...
www.nature.com
November 26, 2024 at 3:11 AM
Our latest review-with @bonniee-leee.bsky.social where we pour our ❤️🧠 & soul to outline our position on why we are so passionate about harnessing the power of sex differences & within sex diff to propel precision medicine forward let us know if we convince you www.nature.com/articles/s41...
Reposted by Lewis Holt
There are lots of us missing, but so many joining so quickly
Please do share, and comment below to be added
go.bsky.app/5f2V6L6
(microglia starter pack also here by @mancusorenzo.bsky.social - bsky.app/starter-pack...)
Please do share, and comment below to be added
go.bsky.app/5f2V6L6
(microglia starter pack also here by @mancusorenzo.bsky.social - bsky.app/starter-pack...)
November 17, 2024 at 7:10 PM
There are lots of us missing, but so many joining so quickly
Please do share, and comment below to be added
go.bsky.app/5f2V6L6
(microglia starter pack also here by @mancusorenzo.bsky.social - bsky.app/starter-pack...)
Please do share, and comment below to be added
go.bsky.app/5f2V6L6
(microglia starter pack also here by @mancusorenzo.bsky.social - bsky.app/starter-pack...)
Not sure I have any good pictures in the back pocket to beat the calcium imaging from @mathiasgua.bsky.social
Instead for my first post on Bluesky I want to share the CANglia webinar: trainee series!
Instead for my first post on Bluesky I want to share the CANglia webinar: trainee series!
November 15, 2024 at 6:20 PM
Not sure I have any good pictures in the back pocket to beat the calcium imaging from @mathiasgua.bsky.social
Instead for my first post on Bluesky I want to share the CANglia webinar: trainee series!
Instead for my first post on Bluesky I want to share the CANglia webinar: trainee series!