We hope our atlas can go beyond and become a framework to explore how #vascular #aging unfolds across the human body. Explore the data yourself at haeca.derooijlab.org, and check out the rest of the story here: biorxiv.org/content/10.1.... And importantly, let us know what you think! 9/9

Led by Sarah Dobner, this is the very first derooijlab.org production, and we want to thank all lab members and collaborators for their contributions, our host institution @cemm.oeaw.ac.at, as well as our funders. 8/9

5️⃣ Lastly, by integrating the atlas data with in vitro models of induced EC senescence, we uncovered a core set of genes linked to CDKN1A/p21 expression. We validated this across models, tissues and species, to further our understanding of senescence regulation in ECs. 7/9

4️⃣ Age-associated ECM remodeling signatures were linked to increased activity of #mechanotransduction regulators YAP/TAZ and TEAD, particularly in skin, lung, and liver. Immunofluorescence confirmed increased nuclear YAP in aged human skin ECs. 6/9

3️⃣ Venous aging is different:
Vein ECs lose expression of multiple angiogenic regulators with age, while increasing genes linked to extracellular matrix (ECM) remodeling and altered glycosylation, hinting at effects on #angiogenesis, barrier function, and immune trafficking. 5/9

2️⃣ Inflammaging is everywhere:
Across tissues, aging ECs showed increased expression of inflammatory signaling, immune cell adhesion markers, and #senescence-associated factors. 4/9

1️⃣ A human vascular “Rosetta Stone”:
We identified conserved molecular markers for each endothelial subtype across tissues. These can be used to pinpoint arterial, venous, capillary, or lymphatic ECs in any healthy human tissue context. 3/9

By integrating and harmonizing endothelial cells (ECs) from 34 publicly available #single-cell and single-nucleus #RNAseq datasets, we found 🧵(2/9):

All of this in a super supportive research environment in one of the world's most liveable cities!

For further details and to apply, please follow the link below: lnkd.in/d_K4NdJA

Check out our website for more info about our group and ongoing projects: derooijlab.org

Learn more about our research: derooijlab.org
Find out how to apply: cemm.at/phd-program

We make use of state-of-the-art #omics technologies and human in vitro models, including exciting #vesselonchip systems. Wet & dry lab enthusiasts are thus both very welcome to apply! Relevant links 👇