5️⃣ Lastly, by integrating the atlas data with in vitro models of induced EC senescence, we uncovered a core set of genes linked to CDKN1A/p21 expression. We validated this across models, tissues and species, to further our understanding of senescence regulation in ECs. 7/9

4️⃣ Age-associated ECM remodeling signatures were linked to increased activity of #mechanotransduction regulators YAP/TAZ and TEAD, particularly in skin, lung, and liver. Immunofluorescence confirmed increased nuclear YAP in aged human skin ECs. 6/9

3️⃣ Venous aging is different:
Vein ECs lose expression of multiple angiogenic regulators with age, while increasing genes linked to extracellular matrix (ECM) remodeling and altered glycosylation, hinting at effects on #angiogenesis, barrier function, and immune trafficking. 5/9

2️⃣ Inflammaging is everywhere:
Across tissues, aging ECs showed increased expression of inflammatory signaling, immune cell adhesion markers, and #senescence-associated factors. 4/9

1️⃣ A human vascular “Rosetta Stone”:
We identified conserved molecular markers for each endothelial subtype across tissues. These can be used to pinpoint arterial, venous, capillary, or lymphatic ECs in any healthy human tissue context. 3/9

By integrating and harmonizing endothelial cells (ECs) from 34 publicly available #single-cell and single-nucleus #RNAseq datasets, we found 🧵(2/9):

🚨 Exciting Opportunity! 🚨
Join us on June 9th in Brussels for a satellite conference to the 4th MPS World Summit, where we will address advancements & challenges in #aging research using #microphysiological systems and #organoids.
Sign up here: lnkd.in/dCu_yCBr

Looking for a fully funded PhD position in wonderful Vienna? The CeMM PhD program is now open for applications! 🎓 🥼 🧫

We are seeking passionate and driven students interested in helping us understand #endothelial cell heterogeneity in both healthy and pathological #aging. See below for more info 👇

Our new logo (with a little help from 99designs) reflects the heart of our research on #endothelial cell heterogeneity, #aging, and #vascular pathology, blending science with simplicity. We hope you like it as much as we do!

Our new logo (with a little help from 99designs) reflects the heart of our research on #endothelial cell heterogeneity, #aging, and #vascular pathology, blending science with simplicity. We hope you like it as much as we do!