Kaitlyn Kiernan
@kaitlynakiernan.bsky.social
Postdoc in the Sazanov lab at ISTAustria. Using structural biology to find meaning in a world ruled by microbes. 🔬🦠
After DNA cleavage, the PAM-distal product dissociates but the PAM-proximal DNA remains bound. Cas9 reverts almost entirely back to the binary complex but is blocked from binding new targets until the PAM-proximal product is released. Check out the paper for more details!
July 2, 2025 at 11:30 AM
After DNA cleavage, the PAM-distal product dissociates but the PAM-proximal DNA remains bound. Cas9 reverts almost entirely back to the binary complex but is blocked from binding new targets until the PAM-proximal product is released. Check out the paper for more details!
Very excited to share the first story from my postdoc where we uncover the structural basis for slow Cas9 turnover!
We use kinetics-guided cryo-EM to capture 25 conformational states of SpCas9 from a single dataset - including the first look at Cas9 releasing product.
biorxiv.org/content/10.1...
We use kinetics-guided cryo-EM to capture 25 conformational states of SpCas9 from a single dataset - including the first look at Cas9 releasing product.
biorxiv.org/content/10.1...
November 28, 2024 at 12:32 PM
Very excited to share the first story from my postdoc where we uncover the structural basis for slow Cas9 turnover!
We use kinetics-guided cryo-EM to capture 25 conformational states of SpCas9 from a single dataset - including the first look at Cas9 releasing product.
biorxiv.org/content/10.1...
We use kinetics-guided cryo-EM to capture 25 conformational states of SpCas9 from a single dataset - including the first look at Cas9 releasing product.
biorxiv.org/content/10.1...