#microscopymonday video on a multitalented housekeeper in the #brain: Watch amidst #neurons an #astrocyte. The astrocyte phagocytoses debris but is rather stationary compared to the bypassing #microglia cell. #Neurons thrive around the astrocyte and outgrow neurites.

This #microscopymonday is a cleaning day. Watch how a #microglia cell mops up and phagozytoses debris and junk in a very messy neuronal co-culture. BTW, it was a proper cleaning job → the neurons made it and formed nice networks
Phase contrast imaging started at DIV1. One frame per 20 min.

Something for a belayed #FluorescentFriday. #actin dynamics in differentiating neuroblastoma cells. 'It's fascinating to observe the dynamics of #actin in growth cones and filopodia, even though it's merely GFP-actin.
CSU-W1 SoRa Spinning disk #microscopy performed at the AMBIO facility (Charité).

A glimpse on the membrane trafficking part.

For this #FluorescenceFriday: Cortical rat astrocyte in situ expressing the #actin filament reporter Lifeact-GFP. Actin filament illumination reveals the Christmas tree-like arborization and the massive endfoot around a cerebral blood vessel. Tissue clearing, sp8 Leica confocal & Imaris were used.

Here my #FluorescenceFriday contribution, which links my #astrocytes and live imaging starter groups, showing the leading edge of a Drebrin-KO astrocyte: acute Arp2/3 inhibition turns accumulating membrane (green) into tubules. Microtubules in magenta. Published in www.nature.com/articles/s41...