Tomorrow we will celebrate the 10th Anniversary of our AMBIO Imaging Facility. I am very much to see a lot of cutting edge microscopy data.

#microscopymonday video on a multitalented housekeeper in the #brain: Watch amidst #neurons an #astrocyte. The astrocyte phagocytoses debris but is rather stationary compared to the bypassing #microglia cell. #Neurons thrive around the astrocyte and outgrow neurites.

This #microscopymonday is a cleaning day. Watch how a #microglia cell mops up and phagozytoses debris and junk in a very messy neuronal co-culture. BTW, it was a proper cleaning job → the neurons made it and formed nice networks
Phase contrast imaging started at DIV1. One frame per 20 min.

Something for a belayed #FluorescentFriday. #actin dynamics in differentiating neuroblastoma cells. 'It's fascinating to observe the dynamics of #actin in growth cones and filopodia, even though it's merely GFP-actin.
CSU-W1 SoRa Spinning disk #microscopy performed at the AMBIO facility (Charité).

Here are the titles of the talks corresponding to the posted schedule.

Upcoming 'Advanced Bioimaging' Symposium at 19th of November at the Charité to celebrate the 10th anniversary of our AMBIO imaging facility

Back to the roots, where my personal #actin journey started: the Zoological Institute of the @tu-braunschweig.de. It was great to meet old friends at the Farewell party of my PhD supervisor Martin Rothkegel, who sparked my interest in the #cytoskeleton.

A glimpse on the membrane trafficking part.

@leicamicrosystems.bsky.social Spinning Disk workshop: Tomorrow at 10.00 am at the Charité CCO, Berlin. Talks on phase separation of #synapse proteins, cell signaling, infection biology, and #actin in neuroprotective membrane trafficking during #TBI

On May 13th, a @leicamicrosystems.bsky.social Spinning Disk Microscopy workshop will take place at the Charité CCO. I am happy to contribute with a talk on #actin and #membranetrafficking under #TBI conditions. Please join, if you are interested in cool live cell imaging & cell biology.

I am back to one of my favourite places on this planet: Lovely Bristol. 'Looking forward to the reunion with dear old friends and colleagues from @bristoluni.bsky.social times

One of my personal highlights of 2024: the invitation by Magdalena Götz & Lana Sirko to present our #actin & #astrocytes work at @bmc-lmu.bsky.social. What a great experience with many inspiring discussions and marvelous hosts.

For this #FluorescenceFriday: Cortical rat astrocyte in situ expressing the #actin filament reporter Lifeact-GFP. Actin filament illumination reveals the Christmas tree-like arborization and the massive endfoot around a cerebral blood vessel. Tissue clearing, sp8 Leica confocal & Imaris were used.

Something strange & fascinating for a belayed #EMmonday #Microscopymonday : Huge membrane whorls in reactive #astrocytes. Typically observable in foam cells during atherosclerosis. However they occur specifically in Drebrin-KO astrocytes after a mild stab injury. www.nature.com/articles/s41...

Here my #FluorescenceFriday contribution, which links my #astrocytes and live imaging starter groups, showing the leading edge of a Drebrin-KO astrocyte: acute Arp2/3 inhibition turns accumulating membrane (green) into tubules. Microtubules in magenta. Published in www.nature.com/articles/s41...

Little Task for @chatgpt.bsky.social: Generate a scientific illustration of the molecular #Alzheimers pathology with extracellular Abeta and intracellular Tau tangles. Definitly artificial but not intelligent enough yet...