Josh Dickerson
@joshdcryoem.bsky.social
PostDoc in Lucas Lab at UC Berkeley. Formerly PhD student in Russo group at MRC-LMB and Master's student in Garman group at Univ. Oxford.
Pinned
Josh Dickerson
@joshdcryoem.bsky.social
· Apr 23
Reducing the effects of radiation damage in cryo-EM using liquid helium temperatures | PNAS
The physical limit in determining the atomic structure of biological molecules is
radiation damage. In electron cryomicroscopy, there have been num...
www.pnas.org
After years parked at a Polara, I’m excited to share that my PhD work is finally out! We figured out why imaging at liquid helium temperatures wasn’t working—and how to fix it.
🔗 www.pnas.org/doi/10.1073/...
🔗 www.pnas.org/doi/10.1073/...
Reposted by Josh Dickerson
Chris Russo and Anastasiia Gusach have developed a new method to prepare #cryoEM samples that avoids protein damage during freezing
Read more: www2.mrc-lmb.cam.ac.uk/a-new-method...
#LMBResearch
Read more: www2.mrc-lmb.cam.ac.uk/a-new-method...
#LMBResearch
October 22, 2025 at 4:41 PM
Chris Russo and Anastasiia Gusach have developed a new method to prepare #cryoEM samples that avoids protein damage during freezing
Read more: www2.mrc-lmb.cam.ac.uk/a-new-method...
#LMBResearch
Read more: www2.mrc-lmb.cam.ac.uk/a-new-method...
#LMBResearch
Reposted by Josh Dickerson
We are happy to announce that we will have two new scientific groups led by Andrija Sente and Katerina Naydenova soon! If you are interested to join their lab as a PhD Student apply at our @imprs-lm.bsky.social
www.imprs-lm.mpg.de/index.php/jo...
www.imprs-lm.mpg.de/index.php/jo...
October 7, 2025 at 11:35 AM
We are happy to announce that we will have two new scientific groups led by Andrija Sente and Katerina Naydenova soon! If you are interested to join their lab as a PhD Student apply at our @imprs-lm.bsky.social
www.imprs-lm.mpg.de/index.php/jo...
www.imprs-lm.mpg.de/index.php/jo...
Reposted by Josh Dickerson
Improved cryo-EM reconstruction of sub-50 kDa complexes using 2D template matching www.biorxiv.org/content/10.1101/2025.09.11.675606v1 #cryoem
September 17, 2025 at 8:49 AM
Improved cryo-EM reconstruction of sub-50 kDa complexes using 2D template matching www.biorxiv.org/content/10.1101/2025.09.11.675606v1 #cryoem
Happy for this to be out! We have documentation and tutorials here. Please give it a try and let us know what you think. lucaslab-berkeley.github.io/Leopard-EM/
August 30, 2025 at 1:22 AM
Happy for this to be out! We have documentation and tutorials here. Please give it a try and let us know what you think. lucaslab-berkeley.github.io/Leopard-EM/
Reposted by Josh Dickerson
Pulsed-electron illumination does not reduce beam damage for imaging biological macromolecules www.biorxiv.org/content/10.1101/2025.07.29.667395v1 #cryoem
August 2, 2025 at 2:50 PM
Pulsed-electron illumination does not reduce beam damage for imaging biological macromolecules www.biorxiv.org/content/10.1101/2025.07.29.667395v1 #cryoem
Reposted by Josh Dickerson
@joshdcryoem.bsky.social will also be giving a talk about his prior work as a PhD student with Chris Russo developing strategies to take advantage of the reduced radiation damage at liquid Helium temperatures for cryo-EM. You can find their paper here: www.pnas.org/doi/abs/10.1...
Reducing the effects of radiation damage in cryo-EM using liquid helium temperatures | PNAS
The physical limit in determining the atomic structure of biological molecules is
radiation damage. In electron cryomicroscopy, there have been num...
www.pnas.org
July 25, 2025 at 8:12 PM
@joshdcryoem.bsky.social will also be giving a talk about his prior work as a PhD student with Chris Russo developing strategies to take advantage of the reduced radiation damage at liquid Helium temperatures for cryo-EM. You can find their paper here: www.pnas.org/doi/abs/10.1...
Reposted by Josh Dickerson
If you are going to M&M, also see postdoc @joshdcryoem.bsky.social's poster about tilted vs untilted data collection for in situ structural biology www.sciencedirect.com/science/arti....
To tilt or not to tilt? Strategies for in situ cryo-EM data collection
Recent breakthroughs in single-particle cryogenic electron microscopy (cryo-EM) and protein structure prediction have transformed our ability to resol…
www.sciencedirect.com
July 25, 2025 at 8:08 PM
If you are going to M&M, also see postdoc @joshdcryoem.bsky.social's poster about tilted vs untilted data collection for in situ structural biology www.sciencedirect.com/science/arti....
Check out our recent review discussing the merits of tilt series vs single micrographs for in situ cryo-EM! www.sciencedirect.com/science/arti...
To tilt or not to tilt? Strategies for in situ cryo-EM data collection
Recent breakthroughs in single-particle cryogenic electron microscopy (cryo-EM) and protein structure prediction have transformed our ability to resol…
www.sciencedirect.com
June 28, 2025 at 2:04 PM
Check out our recent review discussing the merits of tilt series vs single micrographs for in situ cryo-EM! www.sciencedirect.com/science/arti...
Reposted by Josh Dickerson
At the 2024 IMC meeting, Richard Henderson laid down a challenge. Design a #TEM single-particle #cryoEM lens with 1mm Cc at 100kV. I'm thrilled to reveal our new Arxiv preprint delivering a fully retrofittable pole-piece swap out for the @jeoleurope.bsky.social 1400 TEM.
arxiv.org/abs/2505.11458
arxiv.org/abs/2505.11458
May 20, 2025 at 6:40 AM
At the 2024 IMC meeting, Richard Henderson laid down a challenge. Design a #TEM single-particle #cryoEM lens with 1mm Cc at 100kV. I'm thrilled to reveal our new Arxiv preprint delivering a fully retrofittable pole-piece swap out for the @jeoleurope.bsky.social 1400 TEM.
arxiv.org/abs/2505.11458
arxiv.org/abs/2505.11458
Reposted by Josh Dickerson
In the Bay Area? Love computational cryo-EM/ET?
Come join us for the first edition of FrostByte - a meeting for the local computational cryo-EM methods community!
Details and registration at frostbyte-cryoem.github.io
Thanks to @joshdcryoem.bsky.social and Ariana Peck for help organizing!
Come join us for the first edition of FrostByte - a meeting for the local computational cryo-EM methods community!
Details and registration at frostbyte-cryoem.github.io
Thanks to @joshdcryoem.bsky.social and Ariana Peck for help organizing!
May 12, 2025 at 8:01 PM
In the Bay Area? Love computational cryo-EM/ET?
Come join us for the first edition of FrostByte - a meeting for the local computational cryo-EM methods community!
Details and registration at frostbyte-cryoem.github.io
Thanks to @joshdcryoem.bsky.social and Ariana Peck for help organizing!
Come join us for the first edition of FrostByte - a meeting for the local computational cryo-EM methods community!
Details and registration at frostbyte-cryoem.github.io
Thanks to @joshdcryoem.bsky.social and Ariana Peck for help organizing!
For a shorter read, @mrclmb.bsky.social and @science.org also have articles about the helium story. www.science.org/content/arti...
April 23, 2025 at 1:41 PM
For a shorter read, @mrclmb.bsky.social and @science.org also have articles about the helium story. www.science.org/content/arti...
After years parked at a Polara, I’m excited to share that my PhD work is finally out! We figured out why imaging at liquid helium temperatures wasn’t working—and how to fix it.
🔗 www.pnas.org/doi/10.1073/...
🔗 www.pnas.org/doi/10.1073/...
Reducing the effects of radiation damage in cryo-EM using liquid helium temperatures | PNAS
The physical limit in determining the atomic structure of biological molecules is
radiation damage. In electron cryomicroscopy, there have been num...
www.pnas.org
April 23, 2025 at 4:23 AM
After years parked at a Polara, I’m excited to share that my PhD work is finally out! We figured out why imaging at liquid helium temperatures wasn’t working—and how to fix it.
🔗 www.pnas.org/doi/10.1073/...
🔗 www.pnas.org/doi/10.1073/...
Reposted by Josh Dickerson
Exploring shaped focused ion beams for lamella preparation https://www.biorxiv.org/content/10.1101/2025.04.01.646235v1
April 6, 2025 at 10:47 PM
Exploring shaped focused ion beams for lamella preparation https://www.biorxiv.org/content/10.1101/2025.04.01.646235v1
Reposted by Josh Dickerson
In-Situ High-Resolution Cryo-EM Reconstructions from CEMOVIS https://www.biorxiv.org/content/10.1101/2025.03.29.646093v1
March 31, 2025 at 8:46 AM
In-Situ High-Resolution Cryo-EM Reconstructions from CEMOVIS https://www.biorxiv.org/content/10.1101/2025.03.29.646093v1
Reposted by Josh Dickerson
Foam film vitrification for cryo-EM https://www.biorxiv.org/content/10.1101/2025.03.10.642366v1
March 12, 2025 at 3:50 PM
Foam film vitrification for cryo-EM https://www.biorxiv.org/content/10.1101/2025.03.10.642366v1
I've been wanting to see a paper using off-axis electron holography for low-dose biological imaging since the start of my PhD. Spatially resolved charge measurements👌
Off-axis electron holography of unstained bacteriophages: Toward electrostatic potential measurement of biological samples pubmed.ncbi.nlm.nih.gov/39818354/
January 18, 2025 at 3:31 PM
I've been wanting to see a paper using off-axis electron holography for low-dose biological imaging since the start of my PhD. Spatially resolved charge measurements👌
Reposted by Josh Dickerson
Kexin Zhang et al.: A new statistical metric for robust target detection in cryo-EM using 2D template matching ##3DReconstructionAndImageProcessing##SingleParticleCryoEM##Imaging@umassboston...##IUCrhttps://journals.iucr.org/paper?S2052252524011771
A new statistical metric for robust target detection in cryo-EM using 2D template matching
A novel statistical metric for 2D template matching (2DTM), the 2DTM p-value, has been developed to improve the detection of targets in cryo-EM images under various imaging and sample conditions, particularly for smaller and aspherical targets.
journals.iucr.org
January 18, 2025 at 1:00 AM
Kexin Zhang et al.: A new statistical metric for robust target detection in cryo-EM using 2D template matching ##3DReconstructionAndImageProcessing##SingleParticleCryoEM##Imaging@umassboston...##IUCrhttps://journals.iucr.org/paper?S2052252524011771
Reposted by Josh Dickerson
Laser Flash Melting Cryo-EM Samples to Overcome Preferred Orientation biorxiv.org/cgi/content/short/2024.11.21.624652v1
November 22, 2024 at 8:10 AM
Laser Flash Melting Cryo-EM Samples to Overcome Preferred Orientation biorxiv.org/cgi/content/short/2024.11.21.624652v1