Johan Duchêne
@johanduchene.bsky.social
Dad, Husband & Scientist | Immunology, Inflammation, Chemokines & Atypical ChemoKine Receptors (ACKRs) | Research & Meta-Research | University of Munich (LMU)
Molecular Inflammation Lab
https://duchene.ipek-research.com
Molecular Inflammation Lab
https://duchene.ipek-research.com
Indeed. And I think it is also important to acknowledge when tools are great.
November 14, 2025 at 1:19 PM
Indeed. And I think it is also important to acknowledge when tools are great.
Here is Laflamme's presentation on Antibody Validation as a Scientific Discipline.
www.youtube.com/watch?v=c0Oc...
www.youtube.com/watch?v=c0Oc...
Antibody Validation as a Scientific Discipline: Lessons and Challenges from YCharOS - Carl Laflamme
YouTube video by Antibody Validation
www.youtube.com
October 26, 2025 at 9:02 PM
Here is Laflamme's presentation on Antibody Validation as a Scientific Discipline.
www.youtube.com/watch?v=c0Oc...
www.youtube.com/watch?v=c0Oc...
Reposted by Johan Duchêne
The antibodies don’t work! The race to rid labs of molecules that ruin experiments
Poorly performing antibodies have plagued biomedical sciences for decades. Several fresh initiatives hope to change this.
www.nature.com/articles/d41...
Poorly performing antibodies have plagued biomedical sciences for decades. Several fresh initiatives hope to change this.
www.nature.com/articles/d41...
The antibodies don’t work! The race to rid labs of molecules that ruin experiments
Poorly performing antibodies have plagued biomedical sciences for decades. Several fresh initiatives hope to change this.
www.nature.com
October 26, 2025 at 5:02 PM
The antibodies don’t work! The race to rid labs of molecules that ruin experiments
Poorly performing antibodies have plagued biomedical sciences for decades. Several fresh initiatives hope to change this.
www.nature.com/articles/d41...
Poorly performing antibodies have plagued biomedical sciences for decades. Several fresh initiatives hope to change this.
www.nature.com/articles/d41...
Here is the Antibody Characterization Report for E3 ubiquitinprotein ligase parkin (Parkin)
zenodo.org/records/5747...
More reports are found here: ycharos.com/data/
zenodo.org/records/5747...
More reports are found here: ycharos.com/data/
Antibody Characterization Report for E3 ubiquitin-protein ligase parkin (Parkin)
Head-to-head comparison of available commercial antibodies against E3 ubiquitin-protein ligase parkin (Parkin) by immunoblot (Western blot), immunoprecipitation and immunofluorescence. The following s...
zenodo.org
September 29, 2025 at 8:00 PM
Here is the Antibody Characterization Report for E3 ubiquitinprotein ligase parkin (Parkin)
zenodo.org/records/5747...
More reports are found here: ycharos.com/data/
zenodo.org/records/5747...
More reports are found here: ycharos.com/data/
Yes the website is super new and will be updated every month with new antibodies.
September 29, 2025 at 7:28 PM
Yes the website is super new and will be updated every month with new antibodies.
Sorry to hear that OGA website does not work at Memorial Sloan Kettering.
Maybe try this ycharos.com
All data are available here too, but it is more tedious as you would need to download a report for each protein.
Maybe try this ycharos.com
All data are available here too, but it is more tedious as you would need to download a report for each protein.
Accueil / Home
YCharOS Inc.
YCharOS Inc. est une entreprise canadienne d’intérêt public, dont la mission est de caractériser les anticorps commerciaux disponibles pour chaque protéine humaine, tout en suivant les pr...
ycharos.com
September 29, 2025 at 7:27 PM
Sorry to hear that OGA website does not work at Memorial Sloan Kettering.
Maybe try this ycharos.com
All data are available here too, but it is more tedious as you would need to download a report for each protein.
Maybe try this ycharos.com
All data are available here too, but it is more tedious as you would need to download a report for each protein.
Reposted by Johan Duchêne
We've also switched from mostly transgenic mouse strains to AAV/retrovirus "transgenes". It cut mouse costs by >50%, and makes our work faster and more responsive to reviewer requests. AAVs can even be used to produce depleting antibodies.
www.sciencedirect.com/science/arti...
www.sciencedirect.com/science/arti...
Durable lymphocyte subset elimination upon a single dose of AAV-delivered depletion antibody dissects immune control of chronic viral infection
To interrogate the role of specific immune cells in infection, cancer, and autoimmunity, immunologists commonly use monoclonal depletion antibodies (d…
www.sciencedirect.com
September 3, 2025 at 4:03 PM
We've also switched from mostly transgenic mouse strains to AAV/retrovirus "transgenes". It cut mouse costs by >50%, and makes our work faster and more responsive to reviewer requests. AAVs can even be used to produce depleting antibodies.
www.sciencedirect.com/science/arti...
www.sciencedirect.com/science/arti...