Johan Duchêne
@johanduchene.bsky.social
Dad, Husband & Scientist | Immunology, Inflammation, Chemokines & Atypical ChemoKine Receptors (ACKRs) | Research & Meta-Research | University of Munich (LMU)
Molecular Inflammation Lab
https://duchene.ipek-research.com
Molecular Inflammation Lab
https://duchene.ipek-research.com
CD44 is involved in cell adhesion, migration & signaling.
It is key in inflammation and immune diseases & cancer.
Good news: most commercial CD44 antibodies are highly specific and work well for:
• Western blot
• Immunofluorescence
• Flow cytometry
Full report: zenodo.org/records/1562...
It is key in inflammation and immune diseases & cancer.
Good news: most commercial CD44 antibodies are highly specific and work well for:
• Western blot
• Immunofluorescence
• Flow cytometry
Full report: zenodo.org/records/1562...
November 14, 2025 at 8:06 AM
CD44 is involved in cell adhesion, migration & signaling.
It is key in inflammation and immune diseases & cancer.
Good news: most commercial CD44 antibodies are highly specific and work well for:
• Western blot
• Immunofluorescence
• Flow cytometry
Full report: zenodo.org/records/1562...
It is key in inflammation and immune diseases & cancer.
Good news: most commercial CD44 antibodies are highly specific and work well for:
• Western blot
• Immunofluorescence
• Flow cytometry
Full report: zenodo.org/records/1562...
S1PR1 controls lymphocyte egress & vascular integrity and is a target for multiple sclerosis therapies.
Studying S1PR1 expression using antibodies is key.
Among 9 commercial antibodies tested, only ONE is highly specific for detecting S1PR1.
Full report here: f1000research.com/articles/13-...
Studying S1PR1 expression using antibodies is key.
Among 9 commercial antibodies tested, only ONE is highly specific for detecting S1PR1.
Full report here: f1000research.com/articles/13-...
November 10, 2025 at 12:01 PM
S1PR1 controls lymphocyte egress & vascular integrity and is a target for multiple sclerosis therapies.
Studying S1PR1 expression using antibodies is key.
Among 9 commercial antibodies tested, only ONE is highly specific for detecting S1PR1.
Full report here: f1000research.com/articles/13-...
Studying S1PR1 expression using antibodies is key.
Among 9 commercial antibodies tested, only ONE is highly specific for detecting S1PR1.
Full report here: f1000research.com/articles/13-...
APOE plays a key role in cardiovascular and Alzheimer’s disease.
Reliable tools are needed to study its expression and function.
Among 14 antibodies tested, 4 showed excellent performance.
Pick up the right one.
Report: f1000research.com/articles/12-...
Data summary: onlygoodantibodies.co.uk/28/
Reliable tools are needed to study its expression and function.
Among 14 antibodies tested, 4 showed excellent performance.
Pick up the right one.
Report: f1000research.com/articles/12-...
Data summary: onlygoodantibodies.co.uk/28/
October 30, 2025 at 9:02 AM
APOE plays a key role in cardiovascular and Alzheimer’s disease.
Reliable tools are needed to study its expression and function.
Among 14 antibodies tested, 4 showed excellent performance.
Pick up the right one.
Report: f1000research.com/articles/12-...
Data summary: onlygoodantibodies.co.uk/28/
Reliable tools are needed to study its expression and function.
Among 14 antibodies tested, 4 showed excellent performance.
Pick up the right one.
Report: f1000research.com/articles/12-...
Data summary: onlygoodantibodies.co.uk/28/
ALS is a neurodegenerative disease
C9ORF72 mutation is common
Understanding its biology is key
Laflamme 2019 (10.7554/eLife.48363) showed:
👉Most antibodies were non-specific
👉Specific ones never used
Since then:
❌ Sc-138763: discontinued
✅ Specific ones (GTX634482,GTX632041, ab221137): adopted
C9ORF72 mutation is common
Understanding its biology is key
Laflamme 2019 (10.7554/eLife.48363) showed:
👉Most antibodies were non-specific
👉Specific ones never used
Since then:
❌ Sc-138763: discontinued
✅ Specific ones (GTX634482,GTX632041, ab221137): adopted
October 12, 2025 at 10:42 AM
ALS is a neurodegenerative disease
C9ORF72 mutation is common
Understanding its biology is key
Laflamme 2019 (10.7554/eLife.48363) showed:
👉Most antibodies were non-specific
👉Specific ones never used
Since then:
❌ Sc-138763: discontinued
✅ Specific ones (GTX634482,GTX632041, ab221137): adopted
C9ORF72 mutation is common
Understanding its biology is key
Laflamme 2019 (10.7554/eLife.48363) showed:
👉Most antibodies were non-specific
👉Specific ones never used
Since then:
❌ Sc-138763: discontinued
✅ Specific ones (GTX634482,GTX632041, ab221137): adopted
PARKIN labels damaged mitochondria for recycling
No PARKIN → no cleanup → neurodegeneration
Mutations in PARK2 are common in Parkinson’s disease
Accurate detection of PARKIN is crucial
Problem:
The most used antibodies are not selective
In contrast, some very specific antibodies are not used
No PARKIN → no cleanup → neurodegeneration
Mutations in PARK2 are common in Parkinson’s disease
Accurate detection of PARKIN is crucial
Problem:
The most used antibodies are not selective
In contrast, some very specific antibodies are not used
September 29, 2025 at 7:21 PM
PARKIN labels damaged mitochondria for recycling
No PARKIN → no cleanup → neurodegeneration
Mutations in PARK2 are common in Parkinson’s disease
Accurate detection of PARKIN is crucial
Problem:
The most used antibodies are not selective
In contrast, some very specific antibodies are not used
No PARKIN → no cleanup → neurodegeneration
Mutations in PARK2 are common in Parkinson’s disease
Accurate detection of PARKIN is crucial
Problem:
The most used antibodies are not selective
In contrast, some very specific antibodies are not used
A comparison of mouse vs human tumor microenvironments shows a major difference.
Mouse TMEs have more macrophages, while human TMEs have more T cells.
Chemokine expression patterns are also different.
Learn more: www.biorxiv.org/content/10.1...
Mouse TMEs have more macrophages, while human TMEs have more T cells.
Chemokine expression patterns are also different.
Learn more: www.biorxiv.org/content/10.1...
July 7, 2025 at 5:16 AM
A comparison of mouse vs human tumor microenvironments shows a major difference.
Mouse TMEs have more macrophages, while human TMEs have more T cells.
Chemokine expression patterns are also different.
Learn more: www.biorxiv.org/content/10.1...
Mouse TMEs have more macrophages, while human TMEs have more T cells.
Chemokine expression patterns are also different.
Learn more: www.biorxiv.org/content/10.1...
#FluorescenceFriday & Erythroblast
Erythroblast stimulated with CXCL12, stained for CXCR4 (green) and nuclei (blue).
Captured via Correlative Light & Electron Microscopy by
@jemimaburden.bsky.social
Learn more: scim.ag/4kTxFZz
@antalpedro.bsky.social
@jffoley.bsky.social
@science.org
Erythroblast stimulated with CXCL12, stained for CXCR4 (green) and nuclei (blue).
Captured via Correlative Light & Electron Microscopy by
@jemimaburden.bsky.social
Learn more: scim.ag/4kTxFZz
@antalpedro.bsky.social
@jffoley.bsky.social
@science.org
June 27, 2025 at 2:28 PM
#FluorescenceFriday & Erythroblast
Erythroblast stimulated with CXCL12, stained for CXCR4 (green) and nuclei (blue).
Captured via Correlative Light & Electron Microscopy by
@jemimaburden.bsky.social
Learn more: scim.ag/4kTxFZz
@antalpedro.bsky.social
@jffoley.bsky.social
@science.org
Erythroblast stimulated with CXCL12, stained for CXCR4 (green) and nuclei (blue).
Captured via Correlative Light & Electron Microscopy by
@jemimaburden.bsky.social
Learn more: scim.ag/4kTxFZz
@antalpedro.bsky.social
@jffoley.bsky.social
@science.org
Not all chemokine signaling is about cell migration!
In mouse erythroblasts, CXCL12-bound CXCR4 enters the nucleus to control gene transcription, trigger calcium bursts, and drive red blood cell formation—especially enucleation.
🔗 science.org/doi/10.1126/sc…#chemokinen#CXCR4R4
In mouse erythroblasts, CXCL12-bound CXCR4 enters the nucleus to control gene transcription, trigger calcium bursts, and drive red blood cell formation—especially enucleation.
🔗 science.org/doi/10.1126/sc…#chemokinen#CXCR4R4
June 21, 2025 at 5:11 PM
Not all chemokine signaling is about cell migration!
In mouse erythroblasts, CXCL12-bound CXCR4 enters the nucleus to control gene transcription, trigger calcium bursts, and drive red blood cell formation—especially enucleation.
🔗 science.org/doi/10.1126/sc…#chemokinen#CXCR4R4
In mouse erythroblasts, CXCL12-bound CXCR4 enters the nucleus to control gene transcription, trigger calcium bursts, and drive red blood cell formation—especially enucleation.
🔗 science.org/doi/10.1126/sc…#chemokinen#CXCR4R4
Non-specific #antibodies still plague #reproducibility in biomedical research.
@YCharOS1
tackles this by testing commercial antibodies in knockout cell lines—and sharing the data openly.
Learn more in their online seminar:
May 29, 2025 | 4pm–5pm CEST
tinyurl.com/YCharOs-Semi...
@YCharOS1
tackles this by testing commercial antibodies in knockout cell lines—and sharing the data openly.
Learn more in their online seminar:
May 29, 2025 | 4pm–5pm CEST
tinyurl.com/YCharOs-Semi...
May 8, 2025 at 7:57 PM
Non-specific #antibodies still plague #reproducibility in biomedical research.
@YCharOS1
tackles this by testing commercial antibodies in knockout cell lines—and sharing the data openly.
Learn more in their online seminar:
May 29, 2025 | 4pm–5pm CEST
tinyurl.com/YCharOs-Semi...
@YCharOS1
tackles this by testing commercial antibodies in knockout cell lines—and sharing the data openly.
Learn more in their online seminar:
May 29, 2025 | 4pm–5pm CEST
tinyurl.com/YCharOs-Semi...
AI-generated image crisis in science.
Among these 2 TEM images, only one is true!
Can you tell which one was generated by AI?
Left or right?
@elisabethbik.bsky.social
doi.org/10.1002/ajh....
Among these 2 TEM images, only one is true!
Can you tell which one was generated by AI?
Left or right?
@elisabethbik.bsky.social
doi.org/10.1002/ajh....
May 4, 2025 at 8:38 AM
AI-generated image crisis in science.
Among these 2 TEM images, only one is true!
Can you tell which one was generated by AI?
Left or right?
@elisabethbik.bsky.social
doi.org/10.1002/ajh....
Among these 2 TEM images, only one is true!
Can you tell which one was generated by AI?
Left or right?
@elisabethbik.bsky.social
doi.org/10.1002/ajh....
5th Antibody Validation Meeting.
September, 9-11, 2025.
University of Bath, UK.
A meeting with scientists, industry, and suppliers to discuss best practices and improve research antibody validation.
antibodyvalidation.co.uk
September, 9-11, 2025.
University of Bath, UK.
A meeting with scientists, industry, and suppliers to discuss best practices and improve research antibody validation.
antibodyvalidation.co.uk
May 2, 2025 at 1:35 PM
5th Antibody Validation Meeting.
September, 9-11, 2025.
University of Bath, UK.
A meeting with scientists, industry, and suppliers to discuss best practices and improve research antibody validation.
antibodyvalidation.co.uk
September, 9-11, 2025.
University of Bath, UK.
A meeting with scientists, industry, and suppliers to discuss best practices and improve research antibody validation.
antibodyvalidation.co.uk
Antibodies don't work.
NLRP3 is by far the most studied inflammasome — yet <30% of commercial antibodies were specific.
Only 4 of 14 tested antibodies detected NLRP3 specifically.
Antibodies must be validated before use.
Full report by @ycharos.bsky.social : zenodo.org/records/1162...
NLRP3 is by far the most studied inflammasome — yet <30% of commercial antibodies were specific.
Only 4 of 14 tested antibodies detected NLRP3 specifically.
Antibodies must be validated before use.
Full report by @ycharos.bsky.social : zenodo.org/records/1162...
April 30, 2025 at 6:15 AM
Antibodies don't work.
NLRP3 is by far the most studied inflammasome — yet <30% of commercial antibodies were specific.
Only 4 of 14 tested antibodies detected NLRP3 specifically.
Antibodies must be validated before use.
Full report by @ycharos.bsky.social : zenodo.org/records/1162...
NLRP3 is by far the most studied inflammasome — yet <30% of commercial antibodies were specific.
Only 4 of 14 tested antibodies detected NLRP3 specifically.
Antibodies must be validated before use.
Full report by @ycharos.bsky.social : zenodo.org/records/1162...
Triptych of Bone Marrow Organoid
- White: DAPI (nuclei)
- Orange: CD271 (mesenchymal cells)
- Blue: CD31 (endothelial cells)
BMO derived from human iPSCs enables hematopoiesis studies in vitro.
Note: This BMO had slight damage during preparation.
Photo by Savannah Fairley
#fluorescentfriday
- White: DAPI (nuclei)
- Orange: CD271 (mesenchymal cells)
- Blue: CD31 (endothelial cells)
BMO derived from human iPSCs enables hematopoiesis studies in vitro.
Note: This BMO had slight damage during preparation.
Photo by Savannah Fairley
#fluorescentfriday
February 28, 2025 at 12:20 PM
Triptych of Bone Marrow Organoid
- White: DAPI (nuclei)
- Orange: CD271 (mesenchymal cells)
- Blue: CD31 (endothelial cells)
BMO derived from human iPSCs enables hematopoiesis studies in vitro.
Note: This BMO had slight damage during preparation.
Photo by Savannah Fairley
#fluorescentfriday
- White: DAPI (nuclei)
- Orange: CD271 (mesenchymal cells)
- Blue: CD31 (endothelial cells)
BMO derived from human iPSCs enables hematopoiesis studies in vitro.
Note: This BMO had slight damage during preparation.
Photo by Savannah Fairley
#fluorescentfriday
Why are ACKR1+ intraplaque neovessels key in atherosclerosis?
These venular vessels express CXCL12 that attracts CD8+ T cells via CXCR4 in the plaque
This may unlock new cardiovascular therapy #Atherosclerosis #Chemokines
Read our study here 👇👇👇
www.ahajournals.org/doi/10.1161/...
These venular vessels express CXCL12 that attracts CD8+ T cells via CXCR4 in the plaque
This may unlock new cardiovascular therapy #Atherosclerosis #Chemokines
Read our study here 👇👇👇
www.ahajournals.org/doi/10.1161/...
February 25, 2025 at 5:54 PM
Why are ACKR1+ intraplaque neovessels key in atherosclerosis?
These venular vessels express CXCL12 that attracts CD8+ T cells via CXCR4 in the plaque
This may unlock new cardiovascular therapy #Atherosclerosis #Chemokines
Read our study here 👇👇👇
www.ahajournals.org/doi/10.1161/...
These venular vessels express CXCL12 that attracts CD8+ T cells via CXCR4 in the plaque
This may unlock new cardiovascular therapy #Atherosclerosis #Chemokines
Read our study here 👇👇👇
www.ahajournals.org/doi/10.1161/...
Journal Editor: "What is the mechanism?"
Scientist: "Better than an answer—I have a tremendous amount of data!"
Scientist: "Better than an answer—I have a tremendous amount of data!"
January 30, 2025 at 6:39 PM
Journal Editor: "What is the mechanism?"
Scientist: "Better than an answer—I have a tremendous amount of data!"
Scientist: "Better than an answer—I have a tremendous amount of data!"
I think it worked 👍
December 27, 2024 at 9:56 AM
I think it worked 👍
Thanks.
We have it in this version too.
I personally prefer this one.
yellow: nuclei
blue: mesenchymal cells
We have it in this version too.
I personally prefer this one.
yellow: nuclei
blue: mesenchymal cells
December 14, 2024 at 6:08 PM
Thanks.
We have it in this version too.
I personally prefer this one.
yellow: nuclei
blue: mesenchymal cells
We have it in this version too.
I personally prefer this one.
yellow: nuclei
blue: mesenchymal cells
There are HSPCs that produce mainly granulocytes but also monocytes. Note also the production of MK.
Here is the link to the study www.nature.com/articles/s41...
I am aware of erythroblast islands, where macrophages phagocytose the enucleated nuclei of RBC.
What do macrophages do to granulocytes?
Here is the link to the study www.nature.com/articles/s41...
I am aware of erythroblast islands, where macrophages phagocytose the enucleated nuclei of RBC.
What do macrophages do to granulocytes?
December 14, 2024 at 4:02 PM
There are HSPCs that produce mainly granulocytes but also monocytes. Note also the production of MK.
Here is the link to the study www.nature.com/articles/s41...
I am aware of erythroblast islands, where macrophages phagocytose the enucleated nuclei of RBC.
What do macrophages do to granulocytes?
Here is the link to the study www.nature.com/articles/s41...
I am aware of erythroblast islands, where macrophages phagocytose the enucleated nuclei of RBC.
What do macrophages do to granulocytes?
A Bone Marrow Organoid (BMO) that allows us to study hematopoiesis in vitro.
Blue: nuclei (DAPI)
Orange: endothelial cells (CD31)
#fluorescencefriday #immunosky
Blue: nuclei (DAPI)
Orange: endothelial cells (CD31)
#fluorescencefriday #immunosky
December 13, 2024 at 9:21 PM
A Bone Marrow Organoid (BMO) that allows us to study hematopoiesis in vitro.
Blue: nuclei (DAPI)
Orange: endothelial cells (CD31)
#fluorescencefriday #immunosky
Blue: nuclei (DAPI)
Orange: endothelial cells (CD31)
#fluorescencefriday #immunosky
Why do certain genetic mutations impact leukocyte production or function?
With Bone Marrow Organoids (BMOs), we can now explore this question in vitro!
BMOs, derived from human iPSCs, provide a tool to study hematopoiesis.
Yellow: nuclei
Blue: mesenchymal cells
#fluorescencefriday #ImmunoSky 🧪
With Bone Marrow Organoids (BMOs), we can now explore this question in vitro!
BMOs, derived from human iPSCs, provide a tool to study hematopoiesis.
Yellow: nuclei
Blue: mesenchymal cells
#fluorescencefriday #ImmunoSky 🧪
December 13, 2024 at 9:55 AM
Why do certain genetic mutations impact leukocyte production or function?
With Bone Marrow Organoids (BMOs), we can now explore this question in vitro!
BMOs, derived from human iPSCs, provide a tool to study hematopoiesis.
Yellow: nuclei
Blue: mesenchymal cells
#fluorescencefriday #ImmunoSky 🧪
With Bone Marrow Organoids (BMOs), we can now explore this question in vitro!
BMOs, derived from human iPSCs, provide a tool to study hematopoiesis.
Yellow: nuclei
Blue: mesenchymal cells
#fluorescencefriday #ImmunoSky 🧪