Jason Derks
@jasonderks.bsky.social
Increasing throughput of proteomics @parallelsq.bsky.social
Links to our preprints:
timePlex (multiplexing with time-offsets): www.biorxiv.org/content/10.1...
JMod (search software for joint-modeling): www.biorxiv.org/content/10.1...
PSMtag (novel mass tag for 9-plexDIA): www.biorxiv.org/content/10.1...
timePlex (multiplexing with time-offsets): www.biorxiv.org/content/10.1...
JMod (search software for joint-modeling): www.biorxiv.org/content/10.1...
PSMtag (novel mass tag for 9-plexDIA): www.biorxiv.org/content/10.1...
May 28, 2025 at 10:05 AM
Links to our preprints:
timePlex (multiplexing with time-offsets): www.biorxiv.org/content/10.1...
JMod (search software for joint-modeling): www.biorxiv.org/content/10.1...
PSMtag (novel mass tag for 9-plexDIA): www.biorxiv.org/content/10.1...
timePlex (multiplexing with time-offsets): www.biorxiv.org/content/10.1...
JMod (search software for joint-modeling): www.biorxiv.org/content/10.1...
PSMtag (novel mass tag for 9-plexDIA): www.biorxiv.org/content/10.1...
This work was a collective effort by members of @parallelsq.bsky.social, enabled analytically through the development of specialized search software (JMod) and through mass-tag engineering (PSMtag).
May 28, 2025 at 10:05 AM
This work was a collective effort by members of @parallelsq.bsky.social, enabled analytically through the development of specialized search software (JMod) and through mass-tag engineering (PSMtag).
We paired 3-timePlex with @parallelsq.bsky.social's newly developed mass tag (PSMtag) used for 9-plexDIA.
This enables 27-plex, and we achieve >500 SPD with 25 minutes active chromatography per sample!
This enables 27-plex, and we achieve >500 SPD with 25 minutes active chromatography per sample!
May 28, 2025 at 10:05 AM
We paired 3-timePlex with @parallelsq.bsky.social's newly developed mass tag (PSMtag) used for 9-plexDIA.
This enables 27-plex, and we achieve >500 SPD with 25 minutes active chromatography per sample!
This enables 27-plex, and we achieve >500 SPD with 25 minutes active chromatography per sample!
How have we implemented timePlex?
We stagger and overlap the chromatography of multiple samples in the same run. The time-offsets between samples encode sample-specificity.
We stagger and overlap the chromatography of multiple samples in the same run. The time-offsets between samples encode sample-specificity.
May 28, 2025 at 10:05 AM
How have we implemented timePlex?
We stagger and overlap the chromatography of multiple samples in the same run. The time-offsets between samples encode sample-specificity.
We stagger and overlap the chromatography of multiple samples in the same run. The time-offsets between samples encode sample-specificity.
The time-offsets in timePlex encode samples analogously to mass-offsets in plexDIA.
Time and mass dimensions are orthogonal, which unlocks combinatorial multiplexing when timePlex and plexDIA are used together! This introduces the potential for quadratically increased throughput!
Time and mass dimensions are orthogonal, which unlocks combinatorial multiplexing when timePlex and plexDIA are used together! This introduces the potential for quadratically increased throughput!
May 28, 2025 at 10:05 AM
The time-offsets in timePlex encode samples analogously to mass-offsets in plexDIA.
Time and mass dimensions are orthogonal, which unlocks combinatorial multiplexing when timePlex and plexDIA are used together! This introduces the potential for quadratically increased throughput!
Time and mass dimensions are orthogonal, which unlocks combinatorial multiplexing when timePlex and plexDIA are used together! This introduces the potential for quadratically increased throughput!