Jason Derks
@jasonderks.bsky.social
Increasing throughput of proteomics @parallelsq.bsky.social
We paired 3-timePlex with @parallelsq.bsky.social's newly developed mass tag (PSMtag) used for 9-plexDIA.
This enables 27-plex, and we achieve >500 SPD with 25 minutes active chromatography per sample!
This enables 27-plex, and we achieve >500 SPD with 25 minutes active chromatography per sample!
May 28, 2025 at 10:05 AM
We paired 3-timePlex with @parallelsq.bsky.social's newly developed mass tag (PSMtag) used for 9-plexDIA.
This enables 27-plex, and we achieve >500 SPD with 25 minutes active chromatography per sample!
This enables 27-plex, and we achieve >500 SPD with 25 minutes active chromatography per sample!
How have we implemented timePlex?
We stagger and overlap the chromatography of multiple samples in the same run. The time-offsets between samples encode sample-specificity.
We stagger and overlap the chromatography of multiple samples in the same run. The time-offsets between samples encode sample-specificity.
May 28, 2025 at 10:05 AM
How have we implemented timePlex?
We stagger and overlap the chromatography of multiple samples in the same run. The time-offsets between samples encode sample-specificity.
We stagger and overlap the chromatography of multiple samples in the same run. The time-offsets between samples encode sample-specificity.
The time-offsets in timePlex encode samples analogously to mass-offsets in plexDIA.
Time and mass dimensions are orthogonal, which unlocks combinatorial multiplexing when timePlex and plexDIA are used together! This introduces the potential for quadratically increased throughput!
Time and mass dimensions are orthogonal, which unlocks combinatorial multiplexing when timePlex and plexDIA are used together! This introduces the potential for quadratically increased throughput!
May 28, 2025 at 10:05 AM
The time-offsets in timePlex encode samples analogously to mass-offsets in plexDIA.
Time and mass dimensions are orthogonal, which unlocks combinatorial multiplexing when timePlex and plexDIA are used together! This introduces the potential for quadratically increased throughput!
Time and mass dimensions are orthogonal, which unlocks combinatorial multiplexing when timePlex and plexDIA are used together! This introduces the potential for quadratically increased throughput!
We are excited to introduce ‘time’ as a new domain for proteomics multiplexing!
It enables:
-Label-free multiplexing
-Combinatorial multiplexing with plexDIA
Using combined 9-plexDIA and 3-timePlex we demonstrate 27-plex DIA 🚀
It enables:
-Label-free multiplexing
-Combinatorial multiplexing with plexDIA
Using combined 9-plexDIA and 3-timePlex we demonstrate 27-plex DIA 🚀
May 28, 2025 at 10:05 AM
We are excited to introduce ‘time’ as a new domain for proteomics multiplexing!
It enables:
-Label-free multiplexing
-Combinatorial multiplexing with plexDIA
Using combined 9-plexDIA and 3-timePlex we demonstrate 27-plex DIA 🚀
It enables:
-Label-free multiplexing
-Combinatorial multiplexing with plexDIA
Using combined 9-plexDIA and 3-timePlex we demonstrate 27-plex DIA 🚀