We conclude that blunting of key aspects of exercise-induced skeletal muscle signalling with high carbohydrate availability are not due to the concomitant low fatty acid availability
(7/7)

Check out the paper for more detail: doi.org/10.1113/JP28...

We found that carbohydrate ingestion suppressed exercise-induced skeletal muscle ACC phosphorylation compared with both the fasted state and with niacin ingestion
(6/7)

We confirmed that we were able to suppress fatty acid availability with niacin independent from ingesting carbohydrates and without raising insulin concentrations...
(5/7)

In the current study, we employed a paradigm that can dissociated fat from carbohydrate availability and used this to understand the effects on exercise induced skeletal muscle signalling
(4/7)

We previously found consuming carbohydrate prior to exercise training blunts some skeletal muscle and whole-body adaptations to exercise.

We (and others) also found evidence that the effects may be mediated by fat as much as carbohydrate metabolism
doi.org/10.1210/clin...
(2/7)

New paper from
@CNEM_Bath
in
@JPhysiol
led by
@lfbradshaw

Isolating the effects of carbohydrate and lipid availability on exercise-induced skeletal muscle signalling

doi.org/10.1113/JP28...
(1/7)
#exercise #metabolism #muscle #fasting

New paper!

Do ketone esters affect post-exercise recovery?

doi.org/10.1007/s003...

#ketones #exercise #sports #nutrition #recovery

🚨 3-year Post-doc opportunity at the Centre for Nutrition, Exercise and Metabolism

Opportunity to lead on an RCT involving symbiotic supplementation and ketogenic diets

www.bath.ac.uk/jobs/Vacancy...

Building in our prior work

pubmed.ncbi.nlm.nih.gov/39106867/

Expressing as change from baseline can improve efficiency and area under the curve is a useful summary statistic

See the tables attached for some recommendations on blood sampling dependent on the analyte of interest

What about analysis?

Timing makes a difference to the inferences drawn (also dependent on the analyte of interest, so what about some recommendations?

Depending on the analyte of interest, it may be relevant to consider where blood is sampled from (fingertip capillary, a vein, or an artery)

Note that this will affect the concentrations measured (see figure), depending on net uptake/release by tissues

What about sample timing?

There are various ways of presenting these with different strengths and limitations

What about blood sampling?

Generally these questionnaires ask at least 4 key questions relating to:

Hunger
Fullness
Prospective food consumption
Satiety

How do these get analysed into useful outcomes?...

Mixed-meal tolerance test (MMTT) typically involves ingestion of a meal containing carbohydrate, fat and protein (🍝🧈🍗)

Blood samples and questionnaires are performed before and after ingestion of the meal

What do these questionnaires measure?...

The mixed-meal tolerance test in appetite research
🧵

Topics covered:
-Familiarisation/Standardisation 🟰
-Meal stimulus 🥣
-Appetite scales 😋
-Blood sampling 💉
-Blood analyses 🩸
-Data handling 📊
nature.com/articles/s41...